| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,933,577 | A→T | 10.0% | F190I (TTC→ATC) | PP_2566 ← | leucine‑rich repeat‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,933,577 | 0 | A | T | 10.0% | 113.8 / 5.0 | 40 | F190I (TTC→ATC) | PP_2566 | leucine‑rich repeat‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (19/17); new base T (2/2); total (21/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.49e-01 | |||||||||||
GATCAATGCCTGGACCCTCAAGGCCTCACGGTTCGCTGCAATCGACAGGCGCTGGATTTCAGCGCGATTGGCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCAGCCTCCAGCAAACTGCGTGTTTGCGGCTGGCCTACGGGGCGG > NC_002947/2933434‑2933722 | gATCAATGCCTGGACCCTCAAGGCCTCACGGTTCGCTGCAATCGACAGGCGCTGGATTTCAGCGCGATTGGCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGc > 1:506455/1‑150 (MQ=255) caggtcccTCAAGGCCTCACGGTTCGCTGCAATCGACAGGCGCTGGATTTCAGCGCGATTGGCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAg > 2:192506/6‑150 (MQ=255) cTGGACCCTCAAGGCCTCACGGTTCGCTGCAATCGACAGGCGCTGGATTTCAGCGCGATTGGCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAAAGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCACCGTCAg < 2:594485/150‑1 (MQ=255) ggACCCTCAAGGCCTCACGGTTCGCTGCAATCGACAGGCGCTGGATTTCAGCGCGATTGGCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGc > 2:658344/1‑150 (MQ=255) gTTCGCTGCAATCGACAGGCGCTGGATTTCAGCGCGATTGGCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCt < 1:740999/150‑1 (MQ=255) tGCAATCGACAGGCGCTGGATTTCAGCGCGATTGGCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAgc > 2:425838/1‑150 (MQ=255) tGCAATCGACAGGCGCTGGATTTCAGCGCGATTGGCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAgc > 2:843174/1‑150 (MQ=255) tCGACAGGCGCTGGATTTCAGCGCGATTGGCGCCCTCATGAGCTACCAACAGATTTTCTTGATAACGTTTGATGATCTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGt < 1:192506/150‑1 (MQ=255) cGACAGGCGCTGGATTTCAGCGCGATTGGCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGtt < 1:369642/150‑1 (MQ=255) gACAGGCGCTGGATTTCAGCGCGATTGGCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTg < 1:425838/150‑1 (MQ=255) cAGGCGCTGGATTTCAGCGCGATTGGCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGaa < 1:75128/99‑1 (MQ=255) cAGGCGCTGGATTTCAGCGCGATTGGCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGaa > 2:75128/1‑99 (MQ=255) ttGGCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACt > 2:151595/1‑150 (MQ=255) ggCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCctgct < 1:116273/135‑1 (MQ=255) ggCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCctgct > 2:116273/1‑135 (MQ=255) ggCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTc < 1:151595/150‑1 (MQ=255) cTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCa > 1:341629/1‑150 (MQ=255) cTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCAGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCa < 1:431883/150‑1 (MQ=255) tGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGcccc > 2:297927/1‑150 (MQ=255) aaGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGtt > 2:231137/1‑150 (MQ=255) gCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGaa < 1:237836/145‑1 (MQ=255) gCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGaa > 2:237836/1‑145 (MQ=255) gATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCa < 1:498025/150‑1 (MQ=255) tCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCa > 1:756600/1‑150 (MQ=255) ttGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCAGc < 2:28703/150‑1 (MQ=255) tAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCAGCCTcc > 1:301669/1‑150 (MQ=255) tAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGACCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCAgagatc > 1:450082/1‑145 (MQ=255) aaCGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCa > 2:668466/1‑143 (MQ=255) aaCGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCa < 1:668466/143‑1 (MQ=255) ccAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGc < 2:89205/129‑1 (MQ=255) ccAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGc > 1:89205/1‑129 (MQ=255) cacgcccctggcagGCCTGACGATGGCGGACTTCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCAGCCTCCAGCAAACTGCGTGTTTg < 2:592980‑M1/136‑1 (MQ=255) acgcccctggcagGCCTGACGATGGCGGACTTCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCAGCCTCCAGCAAACTGCGTGTTTGc > 1:592980‑M1/14‑150 (MQ=255) aGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCAGCCTCCAGCAAACTGCGTGTTTGc < 1:843174/150‑1 (MQ=255) cgcCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCAGCCTCCAGCAAACTGCGTGTTTGCgg < 2:756600/150‑1 (MQ=255) aaTGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCAGCCTCCAGCAAACTGCGTGTTTGCGGCTGGCCTa > 1:163845/1‑150 (MQ=255) agGCCTGACGATGGCGGACTTCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTc < 1:8316‑M1/88‑1 (MQ=255) agGCCTGACGATGGCGGACTTCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTc > 2:8316‑M1/3‑90 (MQ=255) TGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCAGCCTCCAGCAAACTGCGTGTTTGCGGCTGGCCTACGGGGc < 1:576220‑M1/150‑1 (MQ=255) ACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACt < 1:711707‑M1/78‑1 (MQ=255) ACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACt > 2:711707‑M1/1‑78 (MQ=255) ACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCAGCCTCCAGCAAACTGCGTGTTTGCGGCTGGCCTACGGGGCgg > 2:44276‑M1/1‑150 (MQ=255) | GATCAATGCCTGGACCCTCAAGGCCTCACGGTTCGCTGCAATCGACAGGCGCTGGATTTCAGCGCGATTGGCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCAGCCTCCAGCAAACTGCGTGTTTGCGGCTGGCCTACGGGGCGG > NC_002947/2933434‑2933722 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |