Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,933,585 A→T 10.3% M187K (ATG→AAG)  PP_2566 ← leucine‑rich repeat‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,933,5850AT10.3% 112.8 / 4.8 39M187K (ATG→AAG) PP_2566leucine‑rich repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (18/17);  new base T (2/2);  total (20/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

CTGGACCCTCAAGGCCTCACGGTTCGCTGCAATCGACAGGCGCTGGATTTCAGCGCGATTGGCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCAGCCTCCAGCAAACTGCGTGTTTGCGGCTGGCCTACGGGGCGG  >  NC_002947/2933443‑2933722
                                                                                                                                              |                                                                                                                                         
caggtcccTCAAGGCCTCACGGTTCGCTGCAATCGACAGGCGCTGGATTTCAGCGCGATTGGCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAg                                                                                                                                    >  2:192506/6‑150 (MQ=255)
cTGGACCCTCAAGGCCTCACGGTTCGCTGCAATCGACAGGCGCTGGATTTCAGCGCGATTGGCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAAAGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCACCGTCAg                                                                                                                                    <  2:594485/150‑1 (MQ=255)
  ggACCCTCAAGGCCTCACGGTTCGCTGCAATCGACAGGCGCTGGATTTCAGCGCGATTGGCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGc                                                                                                                                  >  2:658344/1‑150 (MQ=255)
                     gTTCGCTGCAATCGACAGGCGCTGGATTTCAGCGCGATTGGCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCt                                                                                                               <  1:740999/150‑1 (MQ=255)
                           tGCAATCGACAGGCGCTGGATTTCAGCGCGATTGGCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAgc                                                                                                         >  2:425838/1‑150 (MQ=255)
                           tGCAATCGACAGGCGCTGGATTTCAGCGCGATTGGCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAgc                                                                                                         >  2:843174/1‑150 (MQ=255)
                                tCGACAGGCGCTGGATTTCAGCGCGATTGGCGCCCTCATGAGCTACCAACAGATTTTCTTGATAACGTTTGATGATCTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGt                                                                                                    <  1:192506/150‑1 (MQ=255)
                                 cGACAGGCGCTGGATTTCAGCGCGATTGGCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGtt                                                                                                   <  1:369642/150‑1 (MQ=255)
                                  gACAGGCGCTGGATTTCAGCGCGATTGGCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTg                                                                                                  <  1:425838/150‑1 (MQ=255)
                                                          ttGGCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACt                                                                          >  2:151595/1‑150 (MQ=255)
                                                            ggCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCctgct                                                                                       >  2:116273/1‑135 (MQ=255)
                                                            ggCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCctgct                                                                                       <  1:116273/135‑1 (MQ=255)
                                                            ggCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTc                                                                        <  1:151595/150‑1 (MQ=255)
                                                                 cTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCa                                                                   >  1:341629/1‑150 (MQ=255)
                                                                 cTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCAGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCa                                                                   <  1:431883/150‑1 (MQ=255)
                                                                      tGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGcccc                                                              >  2:297927/1‑150 (MQ=255)
                                                                              aaGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGtt                                                      >  2:231137/1‑150 (MQ=255)
                                                                                gCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGaa                                                         >  2:237836/1‑145 (MQ=255)
                                                                                gCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGaa                                                         <  1:237836/145‑1 (MQ=255)
                                                                                   gATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCa                                                 <  1:498025/150‑1 (MQ=255)
                                                                                        tCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCa                                            >  1:756600/1‑150 (MQ=255)
                                                                                          ttGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCAGc                                          <  2:28703/150‑1 (MQ=255)
                                                                                              tAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCAGCCTcc                                      >  1:301669/1‑150 (MQ=255)
                                                                                              tAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGACCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCAgagatc                                      >  1:450082/1‑145 (MQ=255)
                                                                                               aaCGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCa                                            <  1:668466/143‑1 (MQ=255)
                                                                                               aaCGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCa                                            >  2:668466/1‑143 (MQ=255)
                                                                                                       ccAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGc                                                  <  2:89205/129‑1 (MQ=255)
                                                                                                       ccAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGc                                                  >  1:89205/1‑129 (MQ=255)
                                                                                                               cacgcccctggcagGCCTGACGATGGCGGACTTCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCAGCCTCCAGCAAACTGCGTGTTTg                     <  2:592980‑M1/136‑1 (MQ=255)
                                                                                                                acgcccctggcagGCCTGACGATGGCGGACTTCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCAGCCTCCAGCAAACTGCGTGTTTGc                    >  1:592980‑M1/14‑150 (MQ=255)
                                                                                                                aGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCAGCCTCCAGCAAACTGCGTGTTTGc                    <  1:843174/150‑1 (MQ=255)
                                                                                                                  cgcCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCAGCCTCCAGCAAACTGCGTGTTTGCgg                  <  2:756600/150‑1 (MQ=255)
                                                                                                                          aaTGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCAGCCTCCAGCAAACTGCGTGTTTGCGGCTGGCCTa          >  1:163845/1‑150 (MQ=255)
                                                                                                                           agGCCTGACGATGGCGGACTTCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTc                                                                     <  1:8316‑M1/88‑1 (MQ=255)
                                                                                                                           agGCCTGACGATGGCGGACTTCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTc                                                                     >  2:8316‑M1/3‑90 (MQ=255)
                                                                                                                                TGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCAGCCTCCAGCAAACTGCGTGTTTGCGGCTGGCCTACGGGGc    <  1:576220‑M1/150‑1 (MQ=255)
                                                                                                                                  ACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACt                                                                          <  1:711707‑M1/78‑1 (MQ=255)
                                                                                                                                  ACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACt                                                                          >  2:711707‑M1/1‑78 (MQ=255)
                                                                                                                                  ACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCAGCCTCCAGCAAACTGCGTGTTTGCGGCTGGCCTACGGGGCgg  >  2:44276‑M1/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                         
CTGGACCCTCAAGGCCTCACGGTTCGCTGCAATCGACAGGCGCTGGATTTCAGCGCGATTGGCGCCTTCATGGGTTGCAAGCAGATGGTCTTGATAACGTTTGCCAAGGTCCAGCGCCCGACAATGCCTGACGAAGTCCGCCATCGTCAGGCCTGCCAGGGGCGTGTCGCTGCCAGCGGCGTTGGTGAGCCTGCTGTAAGGACCGACTTCGTCCAGCCCCTCGAAGTTGTGCAGGGCAGCCTCCAGCAAACTGCGTGTTTGCGGCTGGCCTACGGGGCGG  >  NC_002947/2933443‑2933722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑