Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,021,073 A→T 14.3% Q523L (CAG→CTG)  PP_2638 → cellulose synthase operon protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,021,0730AT14.3% 105.4 / 10.7 42Q523L (CAG→CTG) PP_2638cellulose synthase operon protein C
Reads supporting (aligned to +/- strand):  ref base A (21/15);  new base T (4/2);  total (25/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.20e-01

GCGCGAGGACTGGTCGACGGCCAGCCACGTGCTGGGCCAGGCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGACATGCACGCGCTGGAACAGCGTGTTCAGC  >  NC_002947/3020924‑3021222
                                                                                                                                                     |                                                                                                                                                     
gcgtGAGGAGTTGCCGACGGCCAGCCACGTTCTGGGCCAGGCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTgca                                                                                                                                                       <  2:688763/150‑1 (MQ=255)
                        ccaCGTGCTGGGCCAGGCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAAc                                                                                                                               >  2:802646/1‑150 (MQ=255)
                             tGCTGGGCCAGGCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCt                                                                                                                          <  1:380873/150‑1 (MQ=255)
                                    ccAGGCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCaa                                                                                                                   >  2:690505/1‑150 (MQ=255)
                                    ccAGGCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTACGCCaa                                                                                                                   >  1:294110/1‑150 (MQ=255)
                                        gCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTTCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGc                                                                                                               >  1:243023/1‑150 (MQ=255)
                                              gCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTc                                                                                                         >  2:436039/1‑150 (MQ=255)
                                                     cccccGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGt                                                                                                  >  1:304355/1‑150 (MQ=255)
                                                                     gCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCAAGGCTGGAGCAGCACCAGCGACGTGATCCGGCAACGCGCTATGCCAACGGCGTGTTCTTGGAGTACAGCGACCGTGATGc                                                                                  >  2:286794/1‑150 (MQ=255)
                                                                                aGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCCCGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTgg                                                                       <  1:690505/150‑1 (MQ=255)
                                                                                   tGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTTCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGaaa                                                                    <  2:243023/150‑1 (MQ=255)
                                                                                   tGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGaaa                                                                    <  1:436039/150‑1 (MQ=255)
                                                                                     gCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCTGCACCAGcctg                                                                                                                                            <  2:552558/76‑3 (MQ=255)
                                                                                     gCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCTGCACCAGcctg                                                                                                                                            >  1:552558/1‑74 (MQ=255)
                                                                                     gCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCAGCAGCCACGTGATCCGGCAACGCGCTGTGCCAACGGCCTGTTCCTGTAGCCCAGCGACCGTGAGGCCTTGGGTCTGTAAAGt                                                                  >  1:313909/1‑150 (MQ=255)
                                                                                     gCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGt                                                                  >  1:519991/1‑150 (MQ=255)
                                                                                       cAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCCTGCGTCTGGAAAGTCt                                                                >  2:157101/1‑150 (MQ=255)
                                                                                        aaCAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGc                                                                                                               <  1:497240/102‑1 (MQ=255)
                                                                                        aaCAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGc                                                                                                               >  2:497240/1‑102 (MQ=255)
                                                                                        aaCAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTg                                                                                                      <  1:158636/111‑1 (MQ=255)
                                                                                        aaCAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTg                                                                                                      >  2:158636/1‑111 (MQ=255)
                                                                                        aaCAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCtt                                                                               <  1:60451/134‑1 (MQ=255)
                                                                                        aaCAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCtt                                                                               >  2:60451/1‑134 (MQ=255)
                                                                                         aCAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCCACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGa                                                              <  1:489628/150‑1 (MQ=255)
                                                                                             tCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGc                                                                                                               >  1:138080/1‑97 (MQ=255)
                                                                                             tCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGc                                                                                                               <  2:138080/97‑1 (MQ=255)
                                                                                                       cAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGGACCTCAg                                                >  1:728015/1‑150 (MQ=255)
                                                                                                       cAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAAGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGGCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAg                                                >  1:691089/1‑150 (MQ=255)
                                                                                                           ggCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGa                                            <  1:802646/150‑1 (MQ=255)
                                                                                                                 cTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGa                                      <  2:304355/150‑1 (MQ=255)
                                                                                                                                 ggCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGACATGCAcgc                      >  2:569247/1‑150 (MQ=255)
                                                                                                                                         cgtggCTGGTGCTGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGACATGCACGCGCTGGAAc              >  2:720740/4‑150 (MQ=255)
                                                                                                                                          gtggCTGGTGCTGCACCAGCCGCGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGACATGCACGCGCTGGAACa             >  1:789318/3‑150 (MQ=255)
                                                                                                                                          cAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTg                                                                        <  2:841813/91‑1 (MQ=255)
                                                                                                                                          cAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTg                                                                        >  1:841813/1‑91 (MQ=255)
                                                                                                                                          cAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGACATGCACGCGCTGGAACa             >  1:540295/1‑150 (MQ=255)
                                                                                                                                           tggCTGGTGCTGCACCAGCCACGTGATCCGGCAACGCGCTATGCCCACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCgg                                                        <  1:775256/105‑1 (MQ=255)
                                                                                                                                           tggCTGGTGCTGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCgg                                                        >  2:775256/2‑106 (MQ=255)
                                                                                                                                           aGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGACATGCACGCGCTGGAACAg            >  1:719509/1‑150 (MQ=255)
                                                                                                                                            ggCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGACGGCGGTACCTCAGGCTACCTGGAGCGCCGACATGCACGCGCTGGAACAGc           <  1:286794/150‑1 (MQ=255)
                                                                                                                                             gCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGACATGCACGCGCTGGAACAGCg          <  2:698042/150‑1 (MQ=255)
                                                                                                                                               tGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGACCTCGGTGCCTCAGGCGACCTGGAGCGCGGACATGCACGCGCTGGAACAGCgtg        <  1:157101/150‑1 (MQ=255)
                                                                                                                                                ggTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGACATGCACGCGCTGGAACAGCgtgt       >  1:427900/1‑150 (MQ=255)
                                                                                                                                                     agcaCCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGACATGCACGCGCTGGAACAGCGTGTTCAgc  >  2:687724/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCGCGAGGACTGGTCGACGGCCAGCCACGTGCTGGGCCAGGCTTCGGCCCTGGCCCCCGATGAGCCTTGGCTGGTGTATCAGCTGGCCAACAGTCTGCGTCACCAAGGGCGCACTGGCGAGGCCGACGCGGCATTTGCCAGGCTGGTGCAGCACCAGCCACGTGATCCGGCAACGCGCTATGCCAACGGCCTGTTCCTGGAGTCCAGCGACCGTGATGCCTTGGCTCTGGAAAGTCTGAGGGCGGTACCTCAGGCGACCTGGAGCGCGGACATGCACGCGCTGGAACAGCGTGTTCAGC  >  NC_002947/3020924‑3021222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑