| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,069,868 | C→G | 16.7% | S305* (TCA→TGA) | aldB‑II → | aldehyde dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,069,868 | 0 | C | G | 16.7% | 72.9 / 8.7 | 30 | S305* (TCA→TGA) | aldB‑II | aldehyde dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (13/12); new base G (3/2); total (16/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
CGTCGAACTGGGTGGCAAGTCGCCGAACATTTTCTTCGAAGACATCATGCAGGCCGAGCCGGCGTTCATCGAGAAGGCTGCCGAAGGCCTGGTGCTGGCGTTCTTCAACCAGGGCGAGGTGTGCACCTGCCCGTCACGGGCGCTGATCCAGGAGTCGATCTACGAACCGTTCATGGCCGAGGTGATGAAGAAGATCGCCAAGATCACCCGCGGCAACCCGCTGGATACCGAAACCATGGTGGGTGCTCAGGCGTCCGAGCAACAGTACGACAAGATCCTTT > NC_002947/3069734‑3070014 | cGTCGAACTGGGTGGCAAGTCGCCGAACATTTTCTTCGAAGACATCATGCAGGCCGAGCCGGCGTTCATCGAGAAGGCTGCCGAAGGCCTGGTGCTGGCGTTCTTCAACCAGGGCGAGGTGTGCACCTGCCCGTCACGGGCGCTGATCCa < 1:835506/150‑1 (MQ=255)cGTCGAACTGGGTGGCAAGTCGCCGAACATTTTCTTCGAAGACATCATGCAGGCCGAGCCGGCGTTCATCGAGAAGGCTGCCGAAGGCCTGGTGCTGGCGTTCTTCAACCAGGGCGAGGTGTGCACCTGCCCGTCACGGGCGCTGATCCa < 1:658359/150‑1 (MQ=255) aaGTCGCCGAACATTTTCTTCGAAGACATCATGCAGGCCGAGCCGGCGTTCATCGAGAAGGCTGCCGAAGGCCTGGTGCTGGCGTTCTTCAACCAGGGCGAGGTGTGCACCTGCCCGTCACGGGCGCTGATCCAGGAGTCGATCTACGaa < 1:350625/150‑1 (MQ=255) gAACATTTTCTTCGAAGACATCATGCAGGCCGAGCCGGCGTTCATCGAGAAGGCTGCCGAAGGCCTGGTGCTGGCGTTCTTCAACCAGGGCGAGGTGTGCACCTGCCCGTCACGGGCGCTGATCCAGGAGTCGATCTACGAACCGtt < 2:806525/147‑1 (MQ=255) gAACATTTTCTTCGAAGACATCATGCAGGCCGAGCCGGCGTTCATCGAGAAGGCTGCCGAAGGCCTGGTGCTGGCGTTCTTCAACCAGGGCGAGGTGTGCACCTGCCCGTCACGGGCGCTGATCCAGGAGTCGATCTACGAACCGtt > 1:806525/1‑147 (MQ=255) aCATTTTCTTCGAAGACATCATGCAGGCCGAGCCGGCGTTCATCGAGAAGGCTGCCGAAGGCCTGGTGCTGGCGTTCTTCAACCAGGGCGAGGTGTGCACCTGCCCGTCACGGGCGCTGATCCAGGAGTCGATCTACGAACCGTTCATgg < 1:265202/150‑1 (MQ=255) gAAGACATCATGCAGGCCGAGCCGGCGTTCATCGAGAATGCTGCCGAAGGCCTGGTGCTGGCGTTCTTCAACCAGGGCGAGGTGTGCACCTGCCCGTCACGGGCGCTAACGCGGCGGACGTTCGACGAACCGTTCATGGCCGAGGcgatg > 2:496252/1‑150 (MQ=25) gAAGACATCATGCAGGCCGAGCCGGCGTTCATCGAGAAGGCTGCCGAAGGCCTGGTGCTGGCGTTCTTCAACCAGGGCGAGGTGTGCACCTGCCCGTGACGGgcagg > 1:220709/1‑104 (MQ=255) gAAGACATCATGCAGGCCGAGCCGGCGTTCATCGAGAAGGCTGCCGAAGGCCTGGTGCTGGCGTTCTTCAACCAGGGCGAGGTGTGCACCTGCCCGTGACGGgcagg < 2:220709/107‑4 (MQ=255) aaGACATCATGCAGGCCGAGCCGGCGTTCATCGAGAAGGCTGCCGAAGGCCTGGTGCTGGCGTTCTTCAACCAGGGCGAGGTGTGCACCTGCCCGTGACGGgcag < 1:688488/105‑3 (MQ=255) aaGACATCATGCAGGCCGAGCCGGCGTTCATCGAGAAGGCTGCCGAAGGCCTGGTGCTGGCGTTCTTCAACCAGGGCGAGGTGTGCACCTGCCCGTGACGGgcag > 2:688488/1‑103 (MQ=255) ggCCGAGCCGGCGTTCATCGAGAAGGCTGCCGAAGGCCTGGTGCTGGCGTTCTTCATCCAGGGCGAGGTGTGCACCTGCCCGTCACGGGCGCTGATCCAGGAGTCGATCTACGAACCGTTCATGGCCGAGGTGATGAAGAAGATCGCCaa < 2:794828/150‑1 (MQ=255) tCGAGAAGGCTGCCGAAGGCCTGGTGCTGGCGTTCTTCAACCAGGGCGAGGTGTGCACCTGCCCGTCACGGGCGCTGATCCAGGAGTCGATCTACGAACCGTTCATGGCCGGGGTGATGAAGAAGATCGCCAAGATCACCCGCGGCAAcc > 2:528329/1‑150 (MQ=255) gagaAGGCTGCCGAAGGCCTGGTGCTGGCGTTCTTCAACCAGGGCGAGGTGTGCACCTGCCCGTCACGGGCGCTGATCCAGGAGTCGATCTACGAACCGTTCATGGCCGAGGTGATGAAGAAGATCGCCAAGATCACCCGCGGCAACCCg < 1:783339/150‑1 (MQ=255) cTGGTGCTGGCGTTCTTCAACCAGGGCGAGGTGTGCACCTGCCCGTCACGGGCGCTGATCCAGGAGTCGATCTACGAACCGTTCATGGCCGAGGTGATGAAGAAGATCGCCAAGATCACCCGCGGCAACCCGCTGGATACCGAAACCAtg > 1:850776/1‑150 (MQ=255) tGGTGCTGGCGTTCTTCAACCAGGGCGAGGTGTGCACCTGCCCGTCACGGGCGCTGATCCAGGAGTCGATCTACGAACCGTTCATGGCCGAGGTGATGAAGAAGATCGCCAAGATCACCCGCGGCAACCCGCt > 1:648135/1‑133 (MQ=255) tGGTGCTGGCGTTCTTCAACCAGGGCGAGGTGTGCACCTGCCCGTCACGGGCGCTGATCCAGGAGTCGATCTACGAACCGTTCATGGCCGAGGTGATGAAGAAGATCGCCAAGATCACCCGCGGCAACCCGCt < 2:648135/133‑1 (MQ=255) gTTCTTCAACCAGGGCGAGGTGTGCACCTGCCCGTCACGGGCGCTGATCCAGGAGTCGATCTACGAACCGTTCATGGCCGAGGTGATGAAGAAGATCGCCAAGGTc > 1:775789/1‑106 (MQ=255) gTTCTTCAACCAGGGCGAGGTGTGCACCTGCCCGTCACGGGCGCTGATCCAGGAGTCGATCTACGAACCGTTCATGGCCGAGGTGATGAAGAAGATCGCCAAGGTc < 2:775789/106‑1 (MQ=255) ttcAACCAGGGCGAGGTGTGCACCTGCCCGTCACGGGCGCTGATCCAGGAGTCGATCTACGAACCGTTCATGGCCGAGGTGATGAAGAAGATCGCCAAGATCACCCGCGGCAACCCGCt > 2:43098/1‑119 (MQ=255) ttcAACCAGGGCGAGGTGTGCACCTGCCCGTCACGGGCGCTGATCCAGGAGTCGATCTACGAACCGTTCATGGCCGAGGTGATGAAGAAGATCGCCAAGATCACCCGCGGCAACCCGCt < 1:43098/119‑1 (MQ=255) aaCCAGGGCGAGGAGTGCACCTGCCCGTCACGGGCGCTGATCCAGGAGTCGATCTACGAACCGTTCATGGCCGAGGTGATGAAGAAGATCGCCAAGATCACCCGCGGCAACCCGCTGGATACCGAAACCATGGTGGGTGCTCAGGCGTcc > 1:246990/1‑150 (MQ=255) gggCGAGGTGTGCACCTGCCCGTCACGGGCGCTGATCCAGGAGTCGATCTACGAACCGTTCATGGCCGAGGTGATGAAGAAGATCGCCAAGATCAc < 1:816917/96‑1 (MQ=255) gggCGAGGTGTGCACCTGCCCGTCACGGGCGCTGATCCAGGAGTCGATCTACGAACCGTTCATGGCCGAGGTGATGAAGAAGATCGCCAAGATCAc > 2:816917/1‑96 (MQ=255) ggCGAGGTGTGCACCTGCCCGTCACGGGCGCTGATCCAGGAGTCGATCTTCGAACCGTTCATGGCCGAGGTGATGAAGAAGAACGCCAAGATCACCCGGGTCAACCCGCTGGATACCGAAACCATGGTGGGTGCTCAGGCGTCCGAGCaa > 1:141103/1‑150 (MQ=255) gtgtGCACCTGCCCGTCACGGGCGCTGATCCAGGAGTCGATCTACGAACCGTTCATGGCCGAGGTGATGAAGAAGATCGCCAAGATCACCCGCGGCAACCCGCTGGATACCGAAACCATGGTGGGTGCTCAGGCGTCCGAGCAACAGTAc > 2:362682/1‑150 (MQ=255) gcgCCCGTGACGGGCGCTGATCCAGGAGTCGATCTACGAACCGTTCATGGCCGAGGTGATGAAGAAGATCGCCAAGATCACCCGCGGCAACCCGCTGGATACCGAAACCATGGTGGGTGCTCAGGCGTCCGAGCAACAGTACGACAAGAt > 1:679697/3‑150 (MQ=255) cTGCCCGTCACGGGCGCTGATCCAGGAGTCGATCTACGAACCGTTCATGGCCGAGGTGATGAAGAAGATCGCCAAGATCACCCGCGGCAACCCGCTGGATACCGAAACCATGGTGGGTGCTCAGGCGTCCGAGCAACAGTACGACAAGAt > 1:607722/1‑150 (MQ=255) cTGCCCGTCACGGGCGCGGATCCAGGAGTCGATCTACGAACCGTTCATGGCCGAGGTGATGAAGAAGATCGCCAAGATCACCCGCGGCAACCCGCTCGATAAACAAACCATAGTAGGCTAGCACGCGTCCGAACAACATTACGACAAGAt > 2:437143/1‑150 (MQ=255) cGTCACGGGCGCTGATCCAGGAGTCGATCTACGAACCGTTCATGGCCGAGGTGATGAAGAAGATCGCCAAGATCACCCGCGGCAACCCGCTGGATACCGAAACCATGGTGGGTGCTCAGGCGTCCGAGCAACAGTACGACAAGATCCttt < 2:850776/150‑1 (MQ=255) | CGTCGAACTGGGTGGCAAGTCGCCGAACATTTTCTTCGAAGACATCATGCAGGCCGAGCCGGCGTTCATCGAGAAGGCTGCCGAAGGCCTGGTGCTGGCGTTCTTCAACCAGGGCGAGGTGTGCACCTGCCCGTCACGGGCGCTGATCCAGGAGTCGATCTACGAACCGTTCATGGCCGAGGTGATGAAGAAGATCGCCAAGATCACCCGCGGCAACCCGCTGGATACCGAAACCATGGTGGGTGCTCAGGCGTCCGAGCAACAGTACGACAAGATCCTTT > NC_002947/3069734‑3070014 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |