Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,077,153 A→C 7.5% I248S (ATC→AGC)  PP_2687 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,077,1530AC 7.5% 149.9 / 4.8 53I248S (ATC→AGC) PP_2687hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (26/23);  new base C (2/2);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.42e-01

TTAGCCAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAACCGCGG  >  NC_002947/3077013‑3077302
                                                                                                                                            |                                                                                                                                                     
ttAGCCAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCAt                                                                                                                                              <  2:267901/150‑1 (MQ=255)
   tgcAGGGATGCATCCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCa                                                                                                                                           >  1:331769/3‑150 (MQ=255)
      aGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCagcg                                                                                                                                        >  2:621788/1‑150 (MQ=255)
      aGGGATGCAACCCTGCATCGAGGATTTCGTCGATCGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCagcg                                                                                                                                        >  1:495932/1‑150 (MQ=255)
       gggATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCagcga                                                                                                                                       <  2:503334/150‑1 (MQ=255)
           tGCAACCCTTCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGTCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAgcgc                                                                                                                                   <  2:373436/150‑1 (MQ=255)
                   tGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGCTGCATGCCTGCatcgaggaa                                                                                                                                   >  2:629937/1‑139 (MQ=255)
                   tGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGCTGCATGCCTGCatcgaggaa                                                                                                                                   <  1:629937/142‑4 (MQ=255)
                      aTCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCAc                                                                                                                        <  1:79314/150‑1 (MQ=255)
                                 cgtcgATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAAcagca                                                                                                             <  1:516435/150‑1 (MQ=255)
                                        tGCGGTGTAGCGCAGGCGCGCGTTCACTTCGGCGGCCATGTGCTGGGCGGCTCGCACGGCACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGTCCGGCACGTCCACCCGCACCCGCAGCACCTc                                                                                                      <  2:1242/150‑1 (MQ=255)
                                                   gcAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTGCATCCGCAACAGCAGCAACTCGTACAccggcc                                                                                           >  2:262065/1‑150 (MQ=255)
                                                       gcgcgcGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCg                                                                                                     <  2:583985/136‑1 (MQ=255)
                                                       gcgcgcGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCg                                                                                                     >  1:583985/1‑136 (MQ=255)
                                                           gcgTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTg                                                                                   >  1:261276/1‑150 (MQ=255)
                                                              ttCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGt                                                                                >  1:5387/1‑150 (MQ=255)
                                                                cAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTgc                                                                              >  2:33699/1‑150 (MQ=255)
                                                                         ggcCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCCACGCCCGACCATCGGCCAGCGGCTGAGCGCGGGGTGCGGCGCGATa                                                                     >  2:309861/1‑150 (MQ=255)
                                                                            cATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGaca                                                                                                 >  1:282370/1‑119 (MQ=255)
                                                                            cATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGaca                                                                                                 <  2:282370/119‑1 (MQ=255)
                                                                            cATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATa                                                                     <  1:690163/147‑1 (MQ=255)
                                                                            cATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATa                                                                     >  2:690163/1‑147 (MQ=255)
                                                                             aTGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGc                                                                 >  1:826658/1‑150 (MQ=255)
                                                                              tGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCggg                                                                                        <  2:456435/126‑1 (MQ=255)
                                                                              tGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCggg                                                                                        >  1:456435/1‑126 (MQ=255)
                                                                                     ggcggcACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGaa                                                         <  2:5387/150‑1 (MQ=255)
                                                                                     ggcggcACGCCCGGTACTGCCCGGCAGGCCTGCACGGGAGTGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGaa                                                         <  1:262065/150‑1 (MQ=255)
                                                                                       cgccACTCCCGGTACTGCCCGGCAGGCCTGCCAGGATAAGGTCCAGTTCCTCGATGCAGGAATGCAGCGAGCGCGGCACGTTCGCCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAgg                                                       <  2:73570/147‑1 (MQ=255)
                                                                                          cACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCtt                                                    <  1:33699/150‑1 (MQ=255)
                                                                                           aCGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGGAgcattc                                                   <  2:214422/150‑6 (MQ=255)
                                                                                              acaggTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTa                                                >  2:306444/4‑150 (MQ=255)
                                                                                                      tGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCa                                        >  2:352508/1‑150 (MQ=255)
                                                                                                       gCCCGGCAGGCCCGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAg                                       >  2:449099/1‑150 (MQ=255)
                                                                                                                     ccAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGa                                             <  2:78999/130‑1 (MQ=255)
                                                                                                                     ccAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGa                                             >  1:78999/1‑130 (MQ=255)
                                                                                                                     ccAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAggg                         >  2:351728/1‑150 (MQ=255)
                                                                                                                     ccAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAggg                         <  2:826658/150‑1 (MQ=255)
                                                                                                                           tCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGt                                                                                                                            >  2:672204/1‑45 (MQ=255)
                                                                                                                           tCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGt                                                                                                                            <  1:672204/45‑1 (MQ=255)
                                                                                                                           tCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTcc                   >  2:277595/1‑150 (MQ=255)
                                                                                                                             aGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGTACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACAAGGTCAGCGCGCGCAGCAGGGCGCTCCAt                 >  1:332902/1‑150 (MQ=255)
                                                                                                                                tCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGTACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACAAGGTCAGCGCGCGCAGCAGGGCGCTCCATTgg              <  2:332902/150‑1 (MQ=255)
                                                                                                                                tCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTgg              >  1:351633/1‑150 (MQ=255)
                                                                                                                                tCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTgg              <  1:352508/150‑1 (MQ=255)
                                                                                                                                tCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTgg              >  1:190126/1‑150 (MQ=255)
                                                                                                                                    gTTCCTCGATGCAGGCATGCAGCGCGAGCGGCACGTCCACTCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGtaat          <  2:521180/150‑1 (MQ=255)
                                                                                                                                    cgcgcTCGCTGCATGCCTGCATCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTa                                                >  2:842556/5‑112 (MQ=255)
                                                                                                                                    cgcgcTCGCTGCATGCCTGCATCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTa                                                <  1:842556/108‑1 (MQ=255)
                                                                                                                                     ttCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAg                                            <  1:591207/115‑1 (MQ=255)
                                                                                                                                     ttCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGtaata         >  1:521180/1‑150 (MQ=255)
                                                                                                                                     ttCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCAACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAg                                            >  2:591207/1‑115 (MQ=255)
                                                                                                                                      tCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGtaataa        <  2:331769/150‑1 (MQ=255)
                                                                                                                                         tCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAACcg     >  1:611233/1‑150 (MQ=255)
                                                                                                                                            aTGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAACCgcgg  >  1:820196/1‑150 (MQ=255)
                                                                                                                                            |                                                                                                                                                     
TTAGCCAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGGCACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAACCGCGG  >  NC_002947/3077013‑3077302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑