Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,103,512 A→T 15.4% K219M (AAG→ATG)  PP_2713 → two‑component system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,103,5120AT15.4% 56.3 / 5.8 26K219M (AAG→ATG) PP_2713two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base A (13/9);  new base T (2/2);  total (15/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.47e-01

CACACGTGAGCGCCTGACTCAACTGCTGTATGGCTGGGATGAAGAGCCGGAGAGCAACACGCTGGAGGTGAACATCTACCATTTGCGCAAGAAGCTGTTCAACGGCCTGATTCGCACGGTGCGCGGAATTGGCTATCTGGTCGAGGTCAAGGCATGACCTCGCTTCGCCAGCGCACGCTGTGGCGCGTGATGCTCTTGCTGCTACTCGGTACCGGCTTGCTCGCGCTCTATAACTACCACGACAGCAGCCACGAAATTAACGAGGTATATGACGCGCACCTGGCCCAGAACGCGCG  >  NC_002947/3103363‑3103658
                                                                                                                                                     |                                                                                                                                                  
cacaCGTGAGCGCCTGACTCAACTGCTGTATGGCTGGGATGAAGAGCCGGAGAGCAACACGCTGGAGGTGAACATCTACCATTTGCGCAAGAAGCTGTTCAACGGCCTGATTCGCACGGTGCGCGGAATTGGCTATCTGGTCGAGGTCaa                                                                                                                                                    >  2:471382/1‑150 (MQ=255)
 acacGTGAGCGCCTGACTCAACTGCTGTATGGCTGGGATGAAGAGCCGGAGAGCAACACGCTGGAGGTGAACATCTACCATTTGCGCAAGAAGCTGTTCAACGGCCTGATTCGCACGGTGCGCGGAATTGGCTATCTGGTCGAGGTCAAg                                                                                                                                                   <  1:471382/150‑1 (MQ=255)
                                  tGGGATGAAGAGCCGGAGAGCAACACGCTGGAGGTGAACATCTACCATTTGCGCAAGAAGCTGTTCAACGGCCTGATTCGCACGGTGCGCGGAATTGGCTATCTGGTCGAGGTCAAGGCATGACCTCGCTTCGCCAGCGCACGCTGTGgc                                                                                                                  >  1:27002/1‑150 (MQ=255)
                                     gATGAAGAGCCGGAGAGCAACACGCTGGAGGTGAACATCTACCATTTGCGCAAGAAGCTGTTCAACGGCCTGATTCGCACGGTGCGCGGAATTGGCTATCTGGTCGAGGTCAAGGCATGACCTCGCTTCGCCAGCGCACGCTGTGGcgcg                                                                                                               >  1:607252/1‑150 (MQ=255)
                                          agagCCGGAGAGCAACACGCTGGAGGTGAACATCTACCATTTGCGCAAGAAGCTGTTCAACGGCCTGATTCGCACGGTGCGCGGAATTGGCTATCTGGTCGAGGTCATGCCTTGACCTc                                                                                                                                         >  2:85737/1‑119 (MQ=255)
                                          agagCCGGAGAGCAACACGCTGGAGGTGAACATCTACCATTTGCGCAAGAAGCTGTTCAACGGCCTGATTCGCACGGTGCGCGGAATTGGCTATCTGGTCGAGGTCATGCCTTGACCTc                                                                                                                                         <  1:85737/119‑1 (MQ=255)
                                          agagCCGGAGAGCAACACGCTGGAGGTGAACATCTACCATTTGCGCAAGAAGCTGTTCAACGGCCTGATTCGCACGGTGCGCGGAATTGGCTATCTGGTCGAGGTCAAGGCATGACCTCGCTTCGCCAGCGCACGCTGTGGCGCGTGATg                                                                                                          >  1:555470/1‑150 (MQ=255)
                                                ggAGAGCAACACGCTGGAGGTGAACATCTACCATTTGCGCAAGAAGCTGTTCAACGGCCTGATTCGCACGGTGCGCGGAATTGGCTATCTGGTCGAGGTCAAGGCATGACCTCGCTTCGCCAGCGCACGCTGTGGCGCGTGATGCTCTtg                                                                                                    >  1:62432/1‑150 (MQ=255)
                                                    agCAACACGCTGGAGGTGAACATCTACCATTTGCGCAAGAAGCTGTTCAACGGCCTGATTCGCACGGTGCGCGGAATTGGCTATCTGGTCGAGGTCAAGGCATGACCTCGCTTCGCCAGCGCACGCTGTGGCGCGTGATGCTCTtgctgc                                                                                                <  2:62432/150‑1 (MQ=255)
                                                                    tGAACATCTACCATTTGCGCAAGAAGCTGTTCAACGGCCTGATTCGCACGGTGCGCGGAATTGGCTATCTGGTCGAGGTCAAGGCATGACCTCGCTTCGCCAGCGCACGCTGTGGCGCGTGATGCTCTTGCTGCTACTCGGTACCGGCtt                                                                                <  2:555470/150‑1 (MQ=255)
                                                                            tACCATTTGCGCAAGAAGCTGTTCAACGGCCTGATTCGCACGGTGCGCGGAATTGGCTATCTGGTCGAGGTCAAGGCATGACCTCGCTTCGCCAGCGCACGCTGTGGCGCGTGATGCTCTTGCTGCTACTCGGTACCGGCTTGCTCgcgc                                                                        <  1:581057/150‑1 (MQ=255)
                                                                             aCCATTTGCGCAAGAAGCTGTTCAACGGCCTGATTCGCACGGTGCGCGGAATTGGCTATCTGGTCGAGGTCAAGGCATGa                                                                                                                                             <  2:771153/80‑1 (MQ=255)
                                                                             aCCATTTGCGCAAGAAGCTGTTCAACGGCCTGATTCGCACGGTGCGCGGAATTGGCTATCTGGTCGAGGTCAAGGCATGa                                                                                                                                             >  1:771153/1‑80 (MQ=255)
                                                                                        aagaagCTGTTCAACGGCCTGATTCGCACGGTGCGCGGAATTGGCTATCTGGTCGAGGTCAAGGCATGACCTCGCTTCGCCAGCGCACGCTGTGGCGCGTGATGCTCTTGCTGCTACTCGGTACCGGCTTGCTCGCGCTCTATAACTAcc                                                            >  1:241173/1‑150 (MQ=255)
                                                                                            agCTGTTCAACGGCCTGATTCGCACGGTGCGCGGAATTGGCTATCTGGTCGAGGTCAAGGCATGACCTCGCTTCGCCAGCGCACGCTGTGGCGCGTGATGCTCTTGCTGCTACTCGGTACCGGCTTGCTCGCGCTCTATAACTACCACGa                                                        >  2:518266/1‑150 (MQ=255)
                                                                                              ctcttCAACGGCCTGATTCTCACGGTGCGCGCAATTGCCTATCTGGTCCAGGACAAGGCATAACCTCGCTCCGCCAGCGCACGCTGTGGCCCGTGATGATCTTCCTGCAACTCGGTACCGGCTTCCTCGCGCCCTACAACTACCACGAca                                                      <  2:122440/147‑1 (MQ=255)
                                                                                                 ttCAACGGCCTGATTCGCACGGTGCGCGGAATTGGCTATCTGGTCGAGGTCAAGGCATGACCTCGCTTCGCCAGCGCACGCTGTGGCGCGTGATGCTCTTGCTGCTACTc                                                                                           <  1:797107/110‑1 (MQ=255)
                                                                                                 ttCAACGGCCTGATTCGCACGGTGCGCGGAATTGGCTATCTGGTCGAGGTCAAGGCATGACCTCGCTTCGCCAGCGCACGCTGTGGCGCGTGATGCTCTTGCTGCTACTc                                                                                           >  2:797107/1‑110 (MQ=255)
                                                                                                   cAACGGCCTGATTCGCACGGTGCGCGGAATTGGCTATCTGGTCGAGGTCAAGGCATGACCTCGCTTCGCCAGCGCACGCTGTGGCGCGTGATGCTCTTGCTGCTACTCGGTACCGGCTTGCTCGCGCTCTATAACTACCACGACagcagc                                                 <  2:241173/150‑1 (MQ=255)
                                                                                                        gCCTGATTCGCACGGTGCGCGGAATTGGCTATCTGGTCGAGGTCAAGGCATGACCTCGCTTCGCCAGCGCACGCTGTGGCGCGTGATGCTCTTGCTGCTACTCGGTACCGGCTTGCTCGCGCTCTATAACTACCACGACAGCAGCCACGa                                            >  2:469693/1‑150 (MQ=255)
                                                                                                                                       tCTGGTCGAGGTCAAGGCATGACCTCGCTTCGCCAGCGCACGCTGTGGCGCGTGATGCTCTTGCTGCTACTCGGTACCGGCTTGCTCGCGCTCTATAACTACCACGACAGCAGCCACGAAATTAACGAGGTATATGACGCGCACCTGGcc             >  2:577458/1‑150 (MQ=255)
                                                                                                                                       gcgaagcGAGGTCATGCCTTGACCTCGCTTCGCCAGCGCACGCTGTGGCGCGTGATGCTCTTGCTGCTACTCGGTACCGGCTTGCTCGCGCTCTATAACTACCACGACAGCAGCCACGAAATTAACGa                                   <  2:629267/122‑1 (MQ=255)
                                                                                                                                       gcgaagcGAGGTCATGCCTTGACCTCGCTTCGCCAGCGCACGCTGTGGCGCGTGATGCTCTTGCTGCTACTCGGTACCGGCTTGCTCGCGCTCTATAACTACCACGACAGCAGCCACGAAATTAACGa                                   >  1:629267/7‑128 (MQ=255)
                                                                                                                                         tGGTCGAGGTCAAGGCATGACCTCGCTTCGCCAGCGCACGCTGTGGCGCGTGATGCTCTTGCTGCTACTCGGTACCGGCTTGCTCGCGCTCTATAACTACCACGACAGCAGCCACGAAATTAACGAGGTATATGACGCGCACCTGGCCCa           >  1:686310/1‑150 (MQ=255)
                                                                                                                                            tCGAGGTCAAGGCATGACCTCGCTTCGCCAGCGCACGCTGTGGCGCGTGATGCTCTTGCTGCTACTCGGTACCGGCTTGCTCGCGCTCTATAACTACCACGACAGCAGCCACGAAATTAACGAGGTATATGACGCGCACCTGGCCCAGaa        >  2:351453/1‑150 (MQ=255)
                                                                                                                                                ggTCAAGGCATGACCTCGCTTCGCCAGCGCACGCTGTGGCGCGTGATGCTCTTGCTGCTACTCGGTACCGGCTTGCTCGCGCTCTATAACTACCACGACAGCAGCCACGAAATTAACGAGGTATATGACGCGCACCTGGCCCAGAAcgcg    <  1:469693/150‑1 (MQ=255)
                                                                                                                                                  tCAAGGCATGACCTCGCTTCGCCAGCGCACGCTGTGGCGCGTGATGCTCTTGCTGCTACTCGGTACCGGCTTGCTCGCGCTCTATAACTACCACGACAGCAGCCACGAAATTAACGAGGTATATGACGCGCACCTGGCCCAGAAcgcgcg  >  1:42175/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CACACGTGAGCGCCTGACTCAACTGCTGTATGGCTGGGATGAAGAGCCGGAGAGCAACACGCTGGAGGTGAACATCTACCATTTGCGCAAGAAGCTGTTCAACGGCCTGATTCGCACGGTGCGCGGAATTGGCTATCTGGTCGAGGTCAAGGCATGACCTCGCTTCGCCAGCGCACGCTGTGGCGCGTGATGCTCTTGCTGCTACTCGGTACCGGCTTGCTCGCGCTCTATAACTACCACGACAGCAGCCACGAAATTAACGAGGTATATGACGCGCACCTGGCCCAGAACGCGCG  >  NC_002947/3103363‑3103658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: