Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,166,099 G→C 11.7% G163A (GGC→GCC)  PP_2780 → 3‑oxoacyl‑ACP synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,166,0990GC11.7% 114.4 / 2.6 40G163A (GGC→GCC) PP_27803‑oxoacyl‑ACP synthase
Reads supporting (aligned to +/- strand):  ref base G (18/17);  new base C (2/3);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGTTTGCAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTGGACACC  >  NC_002947/3165953‑3166247
                                                                                                                                                  |                                                                                                                                                    
tCGTTTGCCGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGCCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCAt                                                                                                                                                   <  1:185566/150‑1 (MQ=255)
      gCAGGACATGCTGCTCAGCCTGGCGGATAAGCCGGCGCAGGCGCCGAGCTGGGCTCAAGTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGGCAGAGCAACGACCGCCTTGCCCGGGTGATTGCCGAGGCCATCGGCaa                                                                                                                                             <  2:400492/150‑2 (MQ=255)
      gCAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAg                                                                                                                                             >  2:545261/1‑150 (MQ=255)
       cAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGt                                                                                                                                            <  1:545261/150‑1 (MQ=255)
                    tCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGa                                                                                                                               >  2:200306/1‑150 (MQ=255)
                        ccTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCaa                                                                                                                           <  1:466470/150‑1 (MQ=255)
                             cGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTca                                                                                                                      <  1:116086/150‑1 (MQ=255)
                              ggATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACATTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGTCAGTGAAGGCGCGGTGATCAATGTcag                                                                                                                     >  2:345982/1‑150 (MQ=255)
                                    cccGGCGCAGGCGCCGTCCTGGGCTCAATTGATCGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAgcgc                                                                                                               <  1:515879/150‑1 (MQ=255)
                                     ccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTcag                                                                                                                     <  2:536565/143‑1 (MQ=255)
                                     ccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTcag                                                                                                                     >  1:536565/1‑143 (MQ=255)
                                     ccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGcc                                                                                                              >  1:42604/1‑150 (MQ=255)
                                     ccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGCCATCGGCaa                                                                                                                                             <  2:815987/119‑2 (MQ=255)
                                     ccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGCCATCGGCaa                                                                                                                                             <  2:815736/119‑2 (MQ=255)
                                     ccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGCCATCGGCaa                                                                                                                                             >  1:815987/1‑118 (MQ=255)
                                     ccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGCCATCGGCaa                                                                                                                                             >  1:815736/1‑118 (MQ=255)
                                                        gggCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCgg                                                                                                                                                <  1:595270/97‑1 (MQ=255)
                                                        gggCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCgg                                                                                                                                                >  2:595270/1‑97 (MQ=255)
                                                               aTTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGt                                                                                                                                            >  2:473085/1‑94 (MQ=255)
                                                               aTTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGt                                                                                                                                            <  1:473085/94‑1 (MQ=255)
                                                                        gcgACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCAtt                                                                           >  2:194280/1‑150 (MQ=255)
                                                                              ggCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAgat                                                                     >  2:299621/1‑150 (MQ=255)
                                                                                   aGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAgat                                                                     <  1:718893/145‑1 (MQ=255)
                                                                                   aGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAgat                                                                     >  2:718893/1‑145 (MQ=255)
                                                                                      tCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCa                                                                                                                                              <  1:74006/69‑1 (MQ=255)
                                                                                      tCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCa                                                                                                                                              >  2:74006/1‑69 (MQ=255)
                                                                                             tGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAgcgc                                                                                                               <  1:519565/93‑1 (MQ=255)
                                                                                             tGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAgcgc                                                                                                               >  2:519565/1‑93 (MQ=255)
                                                                                                catgtCACTGCGTCAGAGCAAAGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGt                                                   <  2:347348/146‑1 (MQ=255)
                                                                                                 aGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTc                                                  >  1:685863/1‑150 (MQ=255)
                                                                                                        tGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTgg                                           <  1:200306/150‑1 (MQ=255)
                                                                                                               gagCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGc                                    >  2:62116/1‑150 (MQ=255)
                                                                                                                  cAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCgg                                 <  2:23754/150‑1 (MQ=255)
                                                                                                                          gCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCg                         >  1:714805/1‑150 (MQ=255)
                                                                                                                                  cgGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTg                 <  1:345982/150‑1 (MQ=255)
                                                                                                                                     gTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCtt              <  2:204915/150‑1 (MQ=255)
                                                                                                                                         ttGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGcc          >  1:383187/1‑150 (MQ=255)
                                                                                                                                             cGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTGGa      >  2:308295/1‑150 (MQ=255)
                                                                                                                                             cGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTGGa      <  1:62116/150‑1 (MQ=255)
                                                                                                                                              gATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTGGac     >  1:339300/1‑150 (MQ=255)
                                                                                                                                                 ggCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTGGACAcc  <  2:339300/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TCGTTTGCAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTGGACACC  >  NC_002947/3165953‑3166247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑