| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 203,740 | C→G | 11.7% | T3082T (ACC→ACG) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 203,740 | 0 | C | G | 11.7% | 90.5 / 5.7 | 35 | T3082T (ACC→ACG) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base C (11/19); new base G (2/2); total (14/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
CGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACC > NC_002947/203596‑203836 | cGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCC < 1:508198‑M1/150‑1 (MQ=255) gTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCg > 2:160865/1‑150 (MQ=255) cAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACTCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCaa > 2:226986/1‑150 (MQ=255) ccGCCACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCgttggccggcacgg < 1:706604‑M1/150‑15 (MQ=255) gCCACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCAAGTCCCCGAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCgttggccggcacggtg < 1:692356‑M1/150‑17 (MQ=255) ccACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTgg < 1:519694/150‑1 (MQ=37) gacCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCgttggccggcacggtgat < 1:695566‑M1/149‑19 (MQ=255) ctccGTCGGTCACCGAAGGCGGCGAGATCACTCACACCATCACCCTAACCAACAAAGATGGTCTGCCCATGAACAACCACAGGGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCa < 2:183553/148‑1 (MQ=11) ccccGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACTCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCa < 1:226986/150‑1 (MQ=17) cGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTg < 1:474611/150‑1 (MQ=35) tCACCGAAGGCGGCGAGATCACTTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCg > 2:725699/1‑150 (MQ=39) cACCGAAGGCGGCGAGATCACTTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGt < 1:63625/150‑1 (MQ=39) aCCGAAGGCGGCGAGATCACTTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTg > 1:150622/1‑150 (MQ=39) aCCGAAGGCGGCGAGATCACTTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTg > 2:850329/1‑150 (MQ=38) ccGAAGGCGGCGAGATCACTTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGa < 2:572660/150‑1 (MQ=40) gAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCgttggccggcacggtgatgacggtcttgcc > 1:182555‑M1/1‑116 (MQ=255) gAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCgttggccggcacggtgatgacggtcttgcc < 2:182555‑M1/146‑31 (MQ=255) gaTCACTTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACaa < 2:150622/150‑1 (MQ=255) ccATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCg < 1:160865/150‑1 (MQ=39) cATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGTGACGGCAAAACCGTCATCACGGTGCCgttggccggcacggtgatgacgg < 1:118977‑M1/116‑24 (MQ=255) cATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGTGACGGCAAAACCGTCATCACGGTGCCgttggccggcacggtgatgacgg > 2:118977‑M1/1‑93 (MQ=255) cATCACCCTGAACAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGGCGGCAAAACCGTCATAACCGTGACGACCAACGGCACCGTGGGCACTGCAACCGTGACTACCCCGGACAACGTCTACGTCgg > 2:145840/1‑150 (MQ=11) aCCCTGCCCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTAcc < 2:647220/150‑1 (MQ=37) ccAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACAGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCACGGACAACGTCTACGTCGGTACCAACGAcc > 2:306681/1‑150 (MQ=255) caacaaAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACGGTGCCgttggccggcacggtga < 1:287849‑M1/98‑18 (MQ=255) caacaaAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACGGTGCCgttggccggcacggtga > 2:287849‑M1/1‑81 (MQ=255) aacaaAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCACCCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCt < 1:410853/150‑1 (MQ=37) aacaaAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCattagccggcacggtga > 1:748831‑M1/1‑80 (MQ=255) aacaaAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCattagccggcacggtga < 2:748831‑M1/97‑18 (MQ=255) gTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAat < 1:725699/150‑1 (MQ=34) gATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCattagccggcacggtga < 1:300233‑M1/80‑18 (MQ=255) gATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCattagccggcacggtga > 2:300233‑M1/1‑63 (MQ=255) aacaacCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCattagccggcacggtgatga > 2:724790‑M1/1‑59 (MQ=255) aacaacCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCattagccggcacggtgatga < 1:724790‑M1/79‑21 (MQ=255) aacaacCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAAcc > 2:322926/1‑150 (MQ=32) | CGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACC > NC_002947/203596‑203836 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |