| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,189,321 | G→C | 21.3% | L458V (CTG→GTG) | PP_2799 ← | class III aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,189,321 | 0 | G | C | 21.3% | 61.8 / 15.0 | 33 | L458V (CTG→GTG) | PP_2799 | class III aminotransferase |
| Reads supporting (aligned to +/- strand): ref base G (14/12); new base C (4/3); total (18/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.25e-01 | |||||||||||
GGGCCATGATGGTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAA‑GATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGAATGATCAGCCCATGCTTG > NC_002947/3189178‑3189466 | gggCCATGATGGTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCtg > 2:610512/1‑150 (MQ=255) ggCCATGATGGTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCtgt < 1:82656/150‑1 (MQ=255) gatgGTTCCCATGGCACCGAGCCCGATCAAGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAgcgc > 1:157482/1‑150 (MQ=255) atgGTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTCGCTCACAGCACCTGCTGTGAgcaaa < 2:144931/150‑4 (MQ=255) ttCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCa > 2:474502/1‑150 (MQ=255) cccATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAAt < 2:830676/150‑1 (MQ=255) tGGCACCGAGCCCGATCACGGAAATGTCGTACTGGAAGCTTTCATCACTGTGTTCACGCACGTTCAAACCCTCATGATTGTTGTGTTACGTGAGTAGCCAGCAGATGCAGCTTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCgg > 2:252915/1‑149 (MQ=255) gAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTca < 1:610512/150‑1 (MQ=255) gCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTcagc > 1:269573/1‑150 (MQ=255) tCGAACTCGAAGCTTTCATCAATGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAgg > 1:331314/1‑150 (MQ=255) aCTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCt < 2:256929/150‑1 (MQ=255) gAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGa < 2:696986/101‑1 (MQ=255) gAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGa > 1:696986/1‑101 (MQ=255) tcaCTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAA‑GATCGCCTCGa > 2:170587/1‑150 (MQ=255) ggTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAA‑GATCGCCTCGATATCGTCCTGGGTGGTa < 2:157482/150‑1 (MQ=255) tCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTGAGCCAGCGTCCGCTCGAAGGCGCTGAA‑GATCGCCTCGATATCGTCCTGGGTGGTaac > 1:138166/1‑149 (MQ=255) tCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAA‑GATCGCCTCGATATCGTCCTGGGTGGTaaa > 2:220238/1‑149 (MQ=255) aaaCCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCACCTGCTGTGa > 1:44463/1‑70 (MQ=39) aaaCCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCACCTGCTGTGa < 2:44463/70‑1 (MQ=39) aaCCCTCATGATTGTTGTTTGACGTGAGTAGCCATCAGATGCAGGCGTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAAGCCGTCGCGTCAGACAGCGTCCGCTCGAAGGCGCTGAA‑GATCGCCTCGATATCGTCCTGGGTGGTAATCaa < 2:138166/150‑1 (MQ=255) cTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAA‑GATCGCCTCGATATCGTCCTGGGTGGTAATCAAAgga > 1:687945/1‑150 (MQ=255) aGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAA‑GATCGCCTCGATATCGTCCTGGGTGGTAAt > 2:477733/1‑105 (MQ=255) aGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAA‑GATCGCCTCGATATCGTCCTGGGTGGTAAt < 1:477733/105‑1 (MQ=255) ccTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAA‑GATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTc > 2:367019/1‑130 (MQ=255) ccTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAA‑GATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTc < 1:367019/130‑1 (MQ=255) gcgcgCTCACAGCACCTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAA‑GATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGAATGATCAg > 2:486301/5‑150 (MQ=255) cTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCCGCACCATCAGC‑GTATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGAATGATCAg > 2:29640/1‑150 (MQ=255) ttGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAA‑GATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGAATGATCAGcc < 1:38218/150‑1 (MQ=255) ttGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAA‑GATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAAATAACTCGAATGATCAGcc < 1:29640/150‑1 (MQ=255) gcgCTCACAGCACCTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAA‑GATCGCCTCGATATCGTCCTg > 1:666962/3‑91 (MQ=39) gcgCTCACAGCACCTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAA‑GATCGCCTCGATATCGTCCTg < 2:666962/89‑1 (MQ=255) tGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAA‑GATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACCGAAGTCTTTCGTGTCGTAGATGGCGCGAATGATCAGccc < 1:284935/150‑1 (MQ=255) tCACAGCACCTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAA‑GATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGAATGATCAGCCCATg > 1:757729/1‑150 (MQ=255) agcagGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAA‑GATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGAATGATCAGCCCATGCTtg > 1:11731/1‑150 (MQ=255) | GGGCCATGATGGTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAA‑GATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGAATGATCAGCCCATGCTTG > NC_002947/3189178‑3189466 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |