| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,190,303 | G→T | 12.0% | Y130* (TAC→TAA) | PP_2799 ← | class III aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,190,303 | 0 | G | T | 12.0% | 89.3 / 3.1 | 34 | Y130* (TAC→TAA) | PP_2799 | class III aminotransferase |
| Reads supporting (aligned to +/- strand): ref base G (12/17); new base T (2/2); total (14/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
AGGTGATGAACCGGGATGGCCGGCAGATCGAAGTCACGGTGCATCGATGGGATGCCGGTCAGGCTTG‑CTGCGGCGACGGTGGCGCCGTGATAGGCCTGTTGTCGAGAGATGAATTTCTTTTTGGCGGGCCGGCCCAGTGCGTTGTTCACGTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTGAGTTGGTGAAGAACACATGGTTCAGATCACCCGGCGCCAGCGCTGCCAACTTGGCGGCGAGCGCTGCGGCAGGCGCATTGGTCTTGTGGCTGAAGCTGTGGTAG > NC_002947/3190154‑3190450 | aggtGATGAACCGGGATGGCCGGCCGGTTTGATTCACGGTGCATCGATGGGATGCCGGTCAGGCTTG‑CTGCGGCGACGGTGGCGCCGTGATAGGCCTGTTGTCGAGAGATGAATTTCTTTTTGGCGGGCCGGCCCAGTGCGTTGTTCACg > 1:22300/1‑150 (MQ=255) tgaACCGGGATGGCCGGCAGATCGAAGTCACGGTGCATCGATGGGATGCCGGTCAGGCTTG‑CTGCGGCGACGGTGGCGCCGTGATAGGCCTGTTGTCGAGAGATGAATTTCTTTTTGGCGGGCCGGCCCAGTGCGTTGTTCACGTACcac < 2:448826/150‑1 (MQ=255) aaCCGGGATGGCCGGCAGATCGAAGTCACGGTGCATCGATGGGATGCCGGTCAGGCTTG‑CTGCGGCGACGGTGGCGCCGTGATAGGCCTGTTGTCGAGAGATGAATTTCTTTTTGGCGGGCCGGCCCAGTGCGTTGTTCACGTACCacac < 2:273324/150‑1 (MQ=255) gATGGCCGGCAGATCGAAGTCACGGTGCATCGATGGGATGCCGGTCAGGCTTG‑CTGCGGCGACGGTGGCGCCGTGATAGGCCTGTTGTCGAGAGATGAATTTCTTTTTGGCGGGCCGGCCCAGTGCGTTGTTCACATACCACACCAtttt < 1:150570/150‑1 (MQ=255) aTGGCCGGCAGATCGAAGTCACGGTGCATCGATGGGATGCCGGTCAGGCTTG‑CTGCGGCGACGGTGGCGCCGTGATAGGCCTGTTGTCGAGAGATCCATTCCTCTTGTCCGGGCCGGCCCAGTGCGTTGTTCACGTGCCACACCATTTTc < 2:335467/150‑1 (MQ=255) cGGCAGATCGAAGTCACGGTGCATCGATGGGATGCCGGTCAGCCTACACT‑CTTTCACTGTGGCGCCGTGATAGGCCTGTTGTCGAGAGATGAATTTCTTTTTGGCGGGCCGGCCCAGTGCGTTGTTCACGTACCACACCATTTTcaccac < 2:22300/150‑1 (MQ=255) aGATCGAAGTCACGGTGCATCGATGGGATGCCGGTCAGGCTTG‑CTGCGGCGACGGTGGCGCCGTGATAGGCCTGTTGTCGAGAGATGAATTTCTTTTTGGCGGGCCGGCCCAGTGCGTTGTTCACGTACCACACCATTTTCACCACCGaa < 2:489138/150‑1 (MQ=255) gAAGTCACGGTGCATCGATGGGATGCCGGTCAGGCTTG‑CTGCGGCGACGGTGGCGCCGTGATAGGCCTGTTGTCGAGAGATGAATTTCTTTTTGGCGGGCCGGCCCAGTGCGTTGTTCACGTACCACACCATTTTCACCACCGAATCAtt < 2:99346/150‑1 (MQ=255) gTGCATCGATGGGATGCCGGTCAGGCTTG‑CTGCGGCGACGGTGGCGCCGTGATAGGCCTGTTGTCGAGAGATGAATTTCTTTTTGGCGGGCCGGCCCAGTGCGTTGTTCACGTACCACACCATTTTCACCACCGAATCATTGGCCTCGGa > 1:114977/1‑150 (MQ=255) tCGATGGGATGCCGGTCAGGCTTG‑CTGCGGCGACGGTGGCGCCGTGATAGGCCTGTTGTCGAGAGATGAATTTCTTTTTGGCGGGCCGGCCCAGTGCGTTGTTCACGTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTg < 2:494236/150‑1 (MQ=255) tGGGATGCCGGTCAGGCTTG‑CTGCGGCGACGGTGGCGCCGTGATAGGCCTGTTGTCGAGAGATGAATTTCTTTTTGGCGGGCCGGCCCAGTGCGTTGTTCACGTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTGAGtt < 2:114977/150‑1 (MQ=255) tGGGATGCCGGTCAGGCTTG‑CTGCGGCGACGGTGGCGCCGTGATAGGCCTGTTGTCGAGAGATGAATTTCTTTTTGGCGGCCCGGCCCAGTGCGTTGTTCACGTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTGAGtt < 1:741025/150‑1 (MQ=255) aGGCTTG‑CTGCGGCGACGGTGGCGCCGTGATAGGCCTGTTGTCGAGAGATGAATTTCTTTTTGGCGGGCCGGCCCAGTGCGTTGTTCACGTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTGAGTTGGTGAAGAAcaca > 2:724178/1‑150 (MQ=255) gCTTG‑CTGCGGCGACGGTGGCGCCGTGATAGGCCTGTTGTCGAGAGATGAATTTCTTTTTGGCGGGCCGGCCCAGTGCGTTGTTCACGTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTGAGTTGGTGAAGAACACATg > 1:229074/1‑150 (MQ=255) ttG‑CTGCGGCGACGGTGGCGCCGTGATAGGCCTGTTGTCGAGAGATGAATTTCTTTTTGGCGGGCCGGCCCAGTGCGTTGTTCACGTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTGAGTTGGTGAAGAACACATGGt < 1:724178/150‑1 (MQ=255) cggcgACGGTGGCGCCGTGATAGGCCTGTTGTCGAGAGATGAATTTCTTTTTGGCGGGCCGGCCCAGTGCGTTGTTCACGTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTGAGTTGGTGAAGAACACATGGTTCAGAt > 1:59641/1‑150 (MQ=255) cggcgACGGTGGCGCCGTGATAGGCCTGTTGTCGAGAGATGAATTTCTTTTTGGCGGGCCGGCCCAGTGCGTTGTTCACGTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTGAGTTGGTGAAGAACACATGGTTCAGAt > 2:233879/1‑150 (MQ=255) gcgcCGTGATAGGCCTGTTGTCGAGAGATGAATTTCTTTTTGGCGGGCCGGCCCAGTGCGTTGTTCACGTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTGAGTTGGTgaagaa < 2:840196/125‑1 (MQ=255) gcgcCGTGATAGGCCTGTTGTCGAGAGATGAATTTCTTTTTGGCGGGCCGGCCCAGTGCGTTGTTCACGTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTGAGTTGGTgaagaa > 1:840196/1‑125 (MQ=255) aaaGGCCTGTTGTCGAGAGATGAATTTCTTTTTGGCGGGCCGGCCCAGTGCGTTGTTCACGTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTGAGTTGGTGAAGAACACATGGTTCAGATCACCCGGCGCCAGCGCTGc > 2:426390/3‑150 (MQ=255) ccTGTTGTCGAGAGATGAATTTCTTTTTGGCGGGCCGGCCCAGTGCGTTGTTCACGTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTGAGTTGGTGAAGAACACATGGTTCAGATCACCCGGCGCCAGCGCTGCCAACt > 1:829457/1‑150 (MQ=255) tttCTTTTTGGCGGGCCGGCCCAGTGCGTTGTTCACGTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCt > 2:249256/1‑79 (MQ=255) tttCTTTTTGGCGGGCCGGCCCAGTGCGTTGTTCACGTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCt < 1:249256/79‑1 (MQ=255) ccggccCAGTGCGTTGTTCACGTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTGAGTTGGTGAAGAACACATGGTTCAGATCACCCGGCGCCAGCGCTGCCAACTTGGCGGCGAGCGCTGCGGCAGGCGCATTGGTCtt < 2:703214/150‑1 (MQ=255) ggccCAGTGCGTTGTTCACGTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTGAGTTGGTGAAGAACACATGGTTCAGATCACCCGGCGCCAGCGCTGCCAACTTGGCGGCGAGCGCTGCGGCAGGCGCATTGGTCTtgt < 1:702381/150‑1 (MQ=255) cAGTGCGTTGTTCACGTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTGAGTTGGTGAAGAACACATGGTTCAGATCACCCGGCGCCAGCGCTGCCAACTTggcg > 2:436136/1‑115 (MQ=255) cAGTGCGTTGTTCACGTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTGAGTTGGTGAAGAACACATGGTTCAGATCACCCGGCGCCAGCGCTGCCAACTTggcg < 1:436136/115‑1 (MQ=255) tGCGTTGTTCACGTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTGAGTTGGTGAAGAACACATGGTTCAGATCACCCGGCGCCAGCGCTGCCAACTTGGCGGCGAGCGCTGCGGCAGGCGCATTGGTCTTGTGGCTGaa > 1:153582/1‑150 (MQ=255) cGTTGTTCACGTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTGAGTTGGTGAAGAACACATGGtt > 2:335060/1‑76 (MQ=255) cGTTGTTCACGTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTGAGTTGGTGAAGAACACATGGtt < 1:335060/76‑1 (MQ=255) gttgttCACTTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTGAGt < 1:756838/56‑1 (MQ=255) gttgttCACTTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTGAGt < 1:756770/56‑1 (MQ=255) gttgttCACTTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTGAGt > 2:756770/1‑56 (MQ=255) gttgttCACTTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTGAGt > 2:756838/1‑56 (MQ=255) aCGTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTGAGTTGGTGAAGAACACATGGTTCAGATCACCCGGCGCCAGCGCTGCCAACTTGGCGGCGAGCGCTGCGGCAGGCGCATTGGTCTTGTGGCTGAAGCTGTGgttg < 2:229074/150‑3 (MQ=255) | AGGTGATGAACCGGGATGGCCGGCAGATCGAAGTCACGGTGCATCGATGGGATGCCGGTCAGGCTTG‑CTGCGGCGACGGTGGCGCCGTGATAGGCCTGTTGTCGAGAGATGAATTTCTTTTTGGCGGGCCGGCCCAGTGCGTTGTTCACGTACCACACCATTTTCACCACCGAATCATTGGCCTCGGAACCTGAGTTGGTGAAGAACACATGGTTCAGATCACCCGGCGCCAGCGCTGCCAACTTGGCGGCGAGCGCTGCGGCAGGCGCATTGGTCTTGTGGCTGAAGCTGTGGTAG > NC_002947/3190154‑3190450 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |