| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 212,560 | T→A | 10.8% | V6022V (GTT→GTA) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 212,560 | 0 | T | A | 10.8% | 88.3 / 2.9 | 36 | V6022V (GTT→GTA) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base T (14/18); new base A (2/2); total (16/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
TGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGG‑‑TCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGA > NC_002947/212415‑212708 | tGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGG‑‑TCAGCACGTCGGTTACcg < 2:656581/150‑1 (MQ=35) tACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGG‑‑TCAGCACGTCGGTTACcgacga < 2:808653/150‑1 (MQ=35) tACTGCCACTGTCACTGCCCCGAATAACGTCTACGTCGGCAGCAACGACCCTGTGGTGATGTCGATCGCTACCGTTGGCAGTGCGGATGTCGGCAAGTACGAGCAACTGACGGTGAACAAGACGCCGA‑‑CCAGCACGTCGGTTACcgacga > 2:187855/1‑150 (MQ=11) cTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGG‑‑TCAGCACGTCGGTTACCGACGAGc < 1:453171/150‑1 (MQ=35) gCCACTGTCACTGCCCCGCATAACGTCTCCCTCGCCATCAACGATCTTTTCGTTATGTCGATCGCTACCGTTGGGGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGG‑‑TCAGCACGTCGGTTACCGACGAGCCa < 1:695896/150‑1 (MQ=11) tGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGG‑‑TCAGCACGTCGGTTACCGACGAGCCAGGTACGCCggg > 2:570998/1‑150 (MQ=35) cccGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGG‑‑TCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCaa > 2:9867/1‑150 (MQ=35) gATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGG‑‑TCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGaa < 1:548860/150‑1 (MQ=35) tACGTCGTCAGCAACGCCCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGG‑‑TCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTg < 1:367415/150‑1 (MQ=21) aCGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGG‑‑TCAGCACGTCGGTTACGAACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTga > 1:649563/1‑149 (MQ=255) ggCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGG‑‑TCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAg > 2:287310/1‑150 (MQ=34) gcagcaACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGG‑‑TCAGCACGTCGGTAACcgacgtg > 1:636805/1‑111 (MQ=255) gcagcaACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGG‑‑TCAGCACGTCGGTAACcgacgtg < 2:636805/113‑3 (MQ=255) gcaACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGG‑‑TCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCa < 1:9867/150‑1 (MQ=35) aaCGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGG‑‑TCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGc > 1:486153/1‑123 (MQ=37) aaCGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGG‑‑TCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGc < 2:486153/123‑1 (MQ=37) cGACCCTGTCGTGATGTCGATCGATCCCGTTGGCGGTGCGGGTTGCGGCAAGTTCGAGCTACTGACGCTGGAAAACAC‑CAGGACTTTGTCCAT‑TACTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGCCCTGGCCATTGTCACCAt > 1:619802/1‑150 (MQ=2) tGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGG‑‑TCAGCACGTCGGTTACCGACGAGCCAGGCACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCAccgc > 1:544733/1‑150 (MQ=37) tACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGG‑‑TCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGc > 2:718628/1‑150 (MQ=255) gTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGG‑‑TCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCa < 1:579739/134‑1 (MQ=40) gTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGG‑‑TCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCa > 2:579739/1‑134 (MQ=40) gATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGG‑‑TCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCg < 2:619802/150‑1 (MQ=40) ggCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGG‑‑TCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTc > 2:311487/1‑150 (MQ=40) tCGAGCAACTGACGCCAGACAAGACGCCTG‑‑TCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGc < 1:746915/150‑1 (MQ=255) cTGACGCTGGACAAGACGCCGG‑‑TCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAAc < 1:187855/150‑1 (MQ=38) aCAAGACGCCGG‑‑TCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTgg < 1:718628/150‑1 (MQ=35) cAAGACGCCGG‑‑TCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGc > 2:642077/1‑150 (MQ=34) gCCGG‑‑TCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGa > 1:218800/1‑150 (MQ=35) g‑‑TCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGAc > 2:369306/1‑78 (MQ=35) g‑‑TCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGAc < 1:369306/78‑1 (MQ=35) aGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTc < 2:218800/150‑1 (MQ=35) ctcGTCGGTAACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGaa > 2:246018/3‑93 (MQ=255) ctcGTCGGTAACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGaa < 1:246018/91‑1 (MQ=16) cGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCAGGTCGTgg < 1:442249/150‑2 (MQ=21) ggTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCt < 1:311487/150‑1 (MQ=35) ggTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCt < 1:287310/150‑1 (MQ=35) ttACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTTAAGGTCACCATCACCGACGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGa < 2:277377/150‑1 (MQ=18) | TGGGTACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGG‑‑TCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGA > NC_002947/212415‑212708 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |