Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,296,545 G→A 9.6% Q125* (CAG→TAG) ‡ PP_2898 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,296,5450GA 9.6% 130.9 / 5.9 53Q125* (CAG→TAG) ‡PP_2898hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (26/20);  new base A (2/3);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.87e-01

CGCGATGATGTGCAGTGGGTACAGCACCCCTCAATCGCACGGGTAGCTGCTCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCGGGCAAGCGGGCTGTGGGCCCGCTGAAATGTAC  >  NC_002947/3296396‑3296689
                                                                                                                                                     |                                                                                                                                                
cgcgATGATGTGCAGTGGGTACAGCACCCCTCAATCGCACGGGTAGCTGCTCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTg                                                                                                                                                  <  2:290167/150‑1 (MQ=255)
         gtgCAGTGGTTACAGCACCCCTCATTCGCACGGGTAGCTGGTCATCAAACTGGCTCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCCAGCCGttata                                                                                                                                         <  1:458091/150‑4 (MQ=255)
          tgCAGTGGGTACAGCACCCCTCAATCGCACGGGTAGCTGCTCAGCACACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTgcgc                                                                                                                                        >  1:706675/1‑150 (MQ=255)
             aGTGGGTACAGCACCCCTCAATCGCACGGGTAGCTGCTCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTgg                                                                                                                                     >  1:784588/1‑150 (MQ=255)
                             cTCAATCGCACGGGTAGCTGCTCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCgg                                                                                                                     >  1:722880/1‑150 (MQ=255)
                                   cGCACGGGTAGCTGCTCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGAAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGt                                                                                                               >  2:22575/1‑150 (MQ=255)
                                     cACGGGTAGCTGCTCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCAAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCTGTTGCGCTGGCCGCTGACCTGGTCGGCAAAGTgg                                                                                                             <  2:11016/150‑1 (MQ=255)
                                        gggTAGCTGCTCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTggcgg                                                                                                          <  2:448680/150‑1 (MQ=255)
                                              ctgctCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGcc                                                                                                                                               <  2:573686/107‑1 (MQ=255)
                                              ctgctCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGcc                                                                                                                                               >  1:573686/1‑107 (MQ=255)
                                               tgctCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAg                                                                                                                <  2:831405/137‑1 (MQ=255)
                                               tgctCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAg                                                                                                                >  1:831405/1‑137 (MQ=255)
                                                    aGCAAACTTGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTg                                                                                                                                                  >  2:12287/1‑98 (MQ=255)
                                                    aGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTg                                                                                                                                                  <  1:12287/98‑1 (MQ=255)
                                                    aGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTg                                                                                                              <  1:112488/134‑1 (MQ=255)
                                                    aGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTg                                                                                                              >  2:112488/1‑134 (MQ=255)
                                                     gCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAGGTGGCGGCGGTCAAGTAccg                                                                                             >  2:131144/1‑149 (MQ=255)
                                                      cAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCCCATGCTCGGCGGCACCGTTCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTTGCCGTTGCGCTGTCAGATGACCTGGTCGGCCAAGTGACGGCGGTACAGTCCCTg                                                                                            >  1:396668/1‑150 (MQ=255)
                                                             gcgcTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGa                                                                                     <  2:508083/150‑1 (MQ=255)
                                                              cgcTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGAt                                                                                    <  1:818954/150‑1 (MQ=255)
                                                                  aCCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAg                                                                                >  1:385822/1‑150 (MQ=255)
                                                                    ccAGATAGGCTTGAGGGGTGGTGCGCATCGTCGGAGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTg                                                                              <  2:472850/150‑1 (MQ=255)
                                                                    ccAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTg                                                                                                                          >  1:621254/1‑106 (MQ=255)
                                                                    ccAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTg                                                                                                                          <  2:621254/106‑1 (MQ=255)
                                                                    ccAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTg                                                                              >  2:638096/1‑150 (MQ=255)
                                                                            gCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTa                                                                                                     <  1:807769/119‑1 (MQ=255)
                                                                            gCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTa                                                                                                     >  2:807769/1‑119 (MQ=255)
                                                                                                  tCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGttt                                                >  2:683523/1‑150 (MQ=255)
                                                                                                                 gtcAATCGATCATCAGGCACAGCGCGTGCAACGGCCTGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTCCCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCTTGCGCCAGCGTTTGTCGGGCCAGGTCgg                                 <  2:396668/148‑1 (MQ=255)
                                                                                                                 gcCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCgg                                 >  1:398483/1‑150 (MQ=255)
                                                                                                                 gcCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCgg                                 >  2:75871/1‑150 (MQ=255)
                                                                                                                 gcCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCGGGCCGATGACCTGGTCGGAAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCgg                                 >  1:186922/1‑150 (MQ=255)
                                                                                                                  ccAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCggg                                <  1:638096/150‑1 (MQ=255)
                                                                                                                    aaTCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTgg                                                                                           >  1:839002/1‑89 (MQ=255)
                                                                                                                    aaTCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTgg                                                                                           <  2:839002/89‑1 (MQ=255)
                                                                                                                       cGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGGGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCGGGCAAGc                           <  2:319560/150‑1 (MQ=255)
                                                                                                                         aCCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCGGGCAAGCgg                         <  2:784588/150‑1 (MQ=255)
                                                                                                                            agcagGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCGGGCAAGCGGGCt                      >  1:703296/1‑150 (MQ=255)
                                                                                                                             gcagGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCGGGCAAGCGGGCtg                     >  2:376862/1‑150 (MQ=255)
                                                                                                                             gcagGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCGGGCAAGCGGGCtg                     >  2:363667/1‑150 (MQ=255)
                                                                                                                                ggCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTCTGCCTGGCCAGGTCGGGCAAGCGGGCTGTgg                  <  1:22575/150‑1 (MQ=255)
                                                                                                                                   acaGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCGGGCAAGCGGGCTGTGGGcc               <  2:706675/150‑1 (MQ=255)
                                                                                                                                   acaGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCGGGCAAGCGGGCTGTGGGcc               >  2:749789/1‑150 (MQ=255)
                                                                                                                                     aGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCGTGGTCGGCCTGCGCCAGAGTTTGTCGGGCCAGGTCGGGCAAGCGGGATGTGGGCCCg             >  2:165849/1‑150 (MQ=255)
                                                                                                                                          gcgCAACGGCCAGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCgg                                           >  2:91812/3‑115 (MQ=255)
                                                                                                                                          gcgCAACGGCCAGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCgg                                           <  1:91812/113‑1 (MQ=255)
                                                                                                                                           cgCAACGGCCAGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCa                                                      <  1:688773/102‑1 (MQ=255)
                                                                                                                                           cgCAACGGCCAGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCa                                                      >  2:688773/2‑103 (MQ=255)
                                                                                                                                             cAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTgc                                                          >  2:808673/1‑97 (MQ=255)
                                                                                                                                             cAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTgc                                                          <  1:808673/97‑1 (MQ=255)
                                                                                                                                              aaCGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGt                                                                                         >  2:436479/1‑65 (MQ=255)
                                                                                                                                              aaCGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGt                                                                                         <  1:436479/65‑1 (MQ=255)
                                                                                                                                              aaCGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCGGGCAAGCGGGCTGTGGGCCCGCTGAAATGt    >  1:244511/1‑150 (MQ=255)
                                                                                                                                                cGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCACCTAGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCGGGCAAGCGGGCTGTGGGCCCGCTGAAATGTAc  <  1:649807/150‑1 (MQ=255)
                                                                                                                                                    tGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCgg                                           <  2:737953/105‑1 (MQ=255)
                                                                                                                                                    tGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCgg                                           >  1:737953/1‑105 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
CGCGATGATGTGCAGTGGGTACAGCACCCCTCAATCGCACGGGTAGCTGCTCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGCGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCGGGCAAGCGGGCTGTGGGCCCGCTGAAATGTAC  >  NC_002947/3296396‑3296689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑