| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,310,843 | A→G | 11.4% | I172V (ATT→GTT) | gabP‑III → | gamma‑aminobutyrate permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,310,843 | 0 | A | G | 11.4% | 89.2 / 3.4 | 35 | I172V (ATT→GTT) | gabP‑III | gamma‑aminobutyrate permease |
| Reads supporting (aligned to +/- strand): ref base A (14/17); new base G (2/2); total (16/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01 | |||||||||||
TGGTGATCACGTTGCTGCTGACGGCCACCAACCTGTTCAGCGTGAAGAATTACGGTGAGTTCGAGTTTTGGCTGGCACTGGTCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTCGCGCCTGTGGGATACGGGGGGCTTCATGCCCAACGGCTTTGGTGCGGTGCTGAGCGCGATGCTGATCACCATGTTCTCGTTCCTCGGGGCCGAGGTGGTGACCATTG > NC_002947/3310715‑3310990 | tGGTGATCACGTTGCTGCTGACGGCCACCAACCTGTTCAGCGTGAAGAATTACGGTGAGTTCGAGTTTTGGCTGGCACTGGTCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCggggg < 2:208197/150‑1 (MQ=255) gTGATCACGTTGCTGCTGACGGCCACCAACCTGTTCAGCGTGAAGAATTACGGTGAGTTCGAGTTTTGGCTGGCACTGGTCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCGTTTTCGGCCTGTTGCCGGGGGcc > 2:363754/1‑150 (MQ=255) gTGATCACGTTGCTGCTGACGGCCACCAACCTGTTCAGCGTGAAGAATTACGGTGAGTTCGAGTTTTGGCTGGCACTGGTCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCGTTTTCGGCCTGTTGCCGGGGGcc > 2:192243/1‑150 (MQ=255) cGTTGCTGCTGACGGCCACCAACCTGTTCAGCGTGAAGAATTACGGTGAGTTCGAGTTTTGGCTGGCACTGGTCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCa < 1:147442/150‑1 (MQ=255) tgctgACGGCCACCAACCTGTTCAGCGTGAAGAATTACGGTGAGTTCGAGTTTTGGCTGGCACTGGTCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAgcggcg > 1:759485/1‑150 (MQ=255) ctgACGGCCACCAACCTGTTCAGCGTGAAGAATTACGGTGAGTTCGAGTTTTGGCTGGCACTGGTCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGtc > 1:798890/1‑150 (MQ=255) gACGGCCACCAACCTGTTCAGCGTGAAGAATTACGGTGAGTTCGAGTTTTGGCTGGCACTGGTCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGtctc < 1:7228/150‑1 (MQ=255) aCGGCCACCAACCTGTTCAGCGTGAAGAATTACGGTGAGTTCGAGTTTTGGCTGGCACTGGTCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTcg < 2:745846/150‑1 (MQ=255) accaACCTGTTCAGCGTGAAGAATTACGGTGAGTTCGAGTTTTGTCTGGCACTGGTCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTCGCGCCtg > 2:803210/1‑150 (MQ=255) accaACCTGTTCAGCGTGAAGAATTACGGTGAGTTCGAGTTTTGGCTGGCACTGGTCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTCGCGCCtg > 2:92282/1‑150 (MQ=255) aaCCTGTTCAGCGTGAAGAATTACGGTGAGTTCGAGTCTTCTCTGGCACTGGTCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTCGCGCCTGTgg < 1:225568/150‑1 (MQ=255) gTTCAGCGTGAAGAATTACGGTGAGTTCGAGTTTTGGCTGGCACTGGTCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTcgc < 1:319096/137‑1 (MQ=255) gTTCAGCGTGAAGAATTACGGTGAGTTCGAGTTTTGGCTGGCACTGGTCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTcgc > 2:319096/1‑137 (MQ=255) gaagaaTTACGGTGAGTTCGAGTTTTGGCTGGCACTGGTCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTCGCGCCTGTGGGATACGGGGGGCtt > 1:247590/1‑150 (MQ=255) tACGGTGAGTTCGAGTTTTGGCTGGCACTGGTCAAGGTAATCAACAACGTCACGTTCATCGCCCTGGGTGTTCGTGTCGTTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTCGCGCCTGTGGGATACGGGGGGCTTCATGccc < 1:192243/150‑1 (MQ=255) tACGGTGAGTTCGAGTTTTGGCTCGCACTGGTCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCGTTTTCGGCCTGTTGCC‑TCAACCGACTGGCGTCGGTGTCGCGCGCCTGTGGGATACGGGGGGCTTCATGccc < 1:363754/150‑1 (MQ=255) ttCGAGTTTTGGCTGGCACTGGTCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTCGCGCCTGTGGGATACg > 1:146113/1‑126 (MQ=255) ttCGAGTTTTGGCTGGCACTGGTCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTCGCGCCTGTGGGATACg < 2:146113/126‑1 (MQ=255) ttCGAGTTTTGGCTGGCACTGGTCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTCGCGCCTGTGGGATACGGGGGGCTTCATGCCCAACGGCttt < 2:798890/150‑1 (MQ=255) aCTGGTCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCGGGC‑GTCAGCGGCGTCTCGCGCCTGTGGGATACGGGGGGCTTCATGCCCAACGGCttt < 2:331598/133‑1 (MQ=255) aCTGGTCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTCGCGCCTGTGGGATACGGGGGGCTTCATGCCCAACGGCttt > 1:331598/1‑133 (MQ=255) aCTGGTCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTCGCGCCTGTGGGATACGGGGGGCTTCATGCCCAACGGCTTTGGTGCGGTGCTGAgcgc > 2:375601/1‑150 (MQ=255) gTCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGACATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTCGCGCCTGTGGGATACGGGGGGCTTCATGCCCAACGGCTTTGGTGCGGTGCTGAGCGCGa < 1:66462/148‑1 (MQ=255) gTCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGACATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTCGCGCCTGTGGGATACGGGGGGCTTCATGCCCAACGGCTTTGGTGCGGTGCTGAGCGCGa > 2:66462/1‑148 (MQ=255) tCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTCGCGCCTGTGGGATACGGGGGGCTTCATGCCCAACGGCTTTggtgcggtg > 1:804137/1‑137 (MQ=255) tCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTCGCGCCTGTGGGATACGGGGGGCTTCATGCCCAACGGCTTTggtgcggtg < 2:804137/137‑1 (MQ=255) ggtggCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTCGCGCCTGTGGGATACGGGGGGCTTCATGCCCAACGGCTTTGGTGCGGTGCTGAGCGCGATGCTGATCAc < 1:767984/150‑1 (MQ=255) cATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTCGCGCCTGTGGGATACGGGGGGCTTCATGCCCAACGGCTTTGGTGCGGTGCTGAGCGCGATGCTGATCACCATGtt > 2:239280/1‑150 (MQ=255) tCAGCTTCATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTCGCGCCTGTGGGATACGGGGGGCTTCATGCCCAACGGCTTTGGTGCGGTGCTGAGCGCGATGCTGATCACCATGTTCTCGt < 1:803210/150‑1 (MQ=255) cATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTCGCGCCTGTGGGATACGGGGGGCTTCATGCCCAACGGCTTTGGTGCGGTGCTGAGCGCGATGCTGATCACCATGTTCTCGTTCCTCgg < 2:537233/150‑1 (MQ=255) aTCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTCGCGCCTGTGGGATACGGGGGGCTTCATGCCCAACGGCTTTGGTGCGGTGCTGAGCGCGATGCTGATCACCATGTTCTCGTTCCTCggg > 2:302188/1‑150 (MQ=255) tGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTCGCGCCTGTGGGATACGGGGGGCTTCATGCCCAACGGCTTTGGTGCGGTGCTGAGCGCGATGCTGATCACCATGTTCTCGTTCCTCGGGGCCGAgg > 2:447156/1‑150 (MQ=255) ggTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTCGCGCCTGTGGGATACGGGGGGCTTCATGCCCAACGGCTTTGGTGCGGTGCTGAGCGCGATGCTGATCACCATGTTCTCGTTCCTCGGGGCCGAggtg < 1:239280/150‑1 (MQ=255) ttGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTCGCGCCTGTGGGATACGGGGGGCTTCATGCCCAACGGCTTTGGTGCGGTGCTGAGCGCGATGCTGATCACCATGTTCTCGTTCCTCGGGGCCGAGGTGGTGAc < 1:204972/150‑1 (MQ=255) ccATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTCGCGCCTGTGGGATACGGGGGGCTTCATGCCCAACGGCTTTGGTGCGGTGCTGAGCGCGATGCTGATCACCATGTTCTCGTTCCTCGGGGCCGAGGTGGTGACCATtg < 1:447156/150‑1 (MQ=255) | TGGTGATCACGTTGCTGCTGACGGCCACCAACCTGTTCAGCGTGAAGAATTACGGTGAGTTCGAGTTTTGGCTGGCACTGGTCAAGGTGGTGGCCATCGTCAGCTTCATCGCCCTGGGTGTTTGCGCCATTTTCGGCCTGTTGCCGGGGGCCGGC‑GTCAGCGGCGTCTCGCGCCTGTGGGATACGGGGGGCTTCATGCCCAACGGCTTTGGTGCGGTGCTGAGCGCGATGCTGATCACCATGTTCTCGTTCCTCGGGGCCGAGGTGGTGACCATTG > NC_002947/3310715‑3310990 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |