Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,441,611 G→A 14.8% Q109Q (CAG→CAA)  PP_3062 → tail length determination protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,441,6110GA14.8% 56.0 / 3.6 27Q109Q (CAG→CAA) PP_3062tail length determination protein
Reads supporting (aligned to +/- strand):  ref base G (10/13);  new base A (2/2);  total (12/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

GACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCACCAGCAGCTGCAGGCGGGCAAGGAATCGCTCAAGTCGAATATCGGCAGGGCGGTGGTGGCTACGGGCGCCGCTGCTGTGCCGACGATGATCAGCGCGAACTATCAAGCGATCATCCGTGACATCGCGATCAAGGCTGA  >  NC_002947/3441468‑3441760
                                                                                                                                               |                                                                                                                                                     
gACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAg                                                                                                                                                 <  2:609960/150‑1 (MQ=255)
gACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAg                                                                                                                                                 >  2:389680/1‑150 (MQ=255)
gACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAg                                                                                                                                                 >  2:1180/1‑150 (MQ=255)
    gcggcgcggcCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTc                                                                                                                                             >  1:211802/1‑150 (MQ=255)
      ggcgcggcCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCAc                                                                                                                                           <  2:387870/150‑1 (MQ=255)
                                 cgcAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAACTCAAGGGTCACCAGCAGCTGCAGGCGGGCAAGGAATCg                                                                                                                >  2:249338/1‑150 (MQ=255)
                                        tGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCACCAGCAGCTGCAGGCGGGCAAGGAATCGCt                                                                                                              >  1:138483/1‑145 (MQ=255)
                                        tGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCACCAGCAGCTGCAGGCGGGCAAGGAATCGCt                                                                                                              <  2:138483/145‑1 (MQ=255)
                                                   cTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAACTCAAGGGTCACCAGCAGCTGCAGGCGGGCAAGGAATCGCTCAAGTCGAATATCGGc                                                                                              >  2:217326/1‑150 (MQ=255)
                                                                gcAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCACCAGCAGCTGCAGGCGGGCAAGGAATCGCTCAAGTCGAATATCGGCAGGGCGGtggtgg                                                                                 >  2:722846/1‑150 (MQ=255)
                                                                        gggATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCACCAGCAGCTGCAGGCGGGCAAGGAATCGCTCAAGTCGAATATCGGCAGGGCGGTGGTGGCTACGGgc                                                                         <  1:622021/150‑1 (MQ=255)
                                                                               aGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCACCAGCAGCTGCAGGCGGGCAAGGAATCGCTCAAGTCGAATATCGGCAGGGCGGTGGTGGCTACGGGCGCCgctg                                                                  <  1:67400/150‑1 (MQ=255)
                                                                               aGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAACTCAAGGGTCACCAGCAGCTGCAGGCGGGCAAGGAATCGCTCAAGTCGAATATCGGCAGGGCGGTGGTGGCTACGGGCGCCgctg                                                                  <  1:249338/150‑1 (MQ=255)
                                                                                     gTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCACCAGCAGCTGCAGGCGGGCAAGGAATCGCTCAAGTCGAATATCGGCAGGGCGGTGGTGGCTACGGGCGCCGCTGCTGTGc                                                            <  1:214401/150‑1 (MQ=255)
                                                                                         gCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCACCAGCAGCTGCAGGCGGGCAAGGAATCGCTCAAGTCGAATATCGGCAGGGCGGtggtgg                                                                                 <  1:43187/125‑1 (MQ=255)
                                                                                         gCTCGGTCGCGAGCACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCACCAGCAGCTGCAGGCGGGCAAGGAATCGCTCAAGTCGAATATCGGCAGGGCGGtggtgg                                                                                 >  2:43187/1‑125 (MQ=255)
                                                                                           tCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCACCAGCAGCTGCAGGCGGGCAAGGAATCGCTCAAGTCGAATATCGGCAGGGCGGTGGTGGCTACGGGCGCCGCTGCTGTGCcgacga                                                      <  1:1180/150‑1 (MQ=255)
                                                                                              gTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCACCAGCAGCTGCAGGCGGGCAAGGAATCGCTCAAGTCGAATATCGGCAGGGCGGTGGTGGCTACGGGCGCCGCTGCTGTGCCGACgatga                                                   >  1:783927/1‑150 (MQ=255)
                                                                                                     gTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCACCAGCAGCTGCAGGCGGGCAAGGAATCGCTCAAGTCGAATATCGGCAGGGCGGTGGTGGCTACGGGCGCCGCTGCTGTGCCGACGATGATCAgcgc                                            <  1:389680/150‑1 (MQ=255)
                                                                                                          aGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCACCAGCAGCTGCAGGCGGGCaa                                                                                                                       <  2:652266/70‑1 (MQ=255)
                                                                                                          aGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCACCAGCAGCTGCAGGCGGGCaa                                                                                                                       >  1:652266/1‑70 (MQ=255)
                                                                                                                   ggCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCACCAGCAGCTGCAGGCGGGCAAGGAATCGCTCAAGTCGAATATCGGCAGGGCGGTGGTGGCTACGGGCGCCGCTGCTGTGCCGACGATGATCAGCGCGAACTATCacccga                              <  2:214278/150‑6 (MQ=255)
                                                                                                                        gAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCACCAGCAGCTGCAGGCGGGCAAGGAATCGCTCAAGTCGAATATCGGCAGGGCGGTGGTGGCTACGGGCGCCGCTGCTGTGCCGACGATGATCAGCGCGAACTATCAAGCGatcatc                         >  1:327042/1‑150 (MQ=255)
                                                                                                                          ggCGCGGGCCGCCGATCTGCAGCTCAAGGGTCACCAGCAGCTGCAGGCGGGCAAGGAATCGCTCAAGTCGAATATCGGCAGGGCGGTGGTGGCTACGGGCGCCGCTGCTGTGCCGACGATGATCAGCGCGAACTATCAAGCGATCATCCg                       <  2:211802/150‑1 (MQ=255)
                                                                                                                             gcgGGCCGCCGATCTGCAACTCAAGGGTCACCAGCAGCTGCAGGCGGGCAAGGAATCGCTCAAGTCGAATATCGGCAGGGCGGTGGTGGCTACGGGCGCCGCTGCTGTGCCGACGATGATCAGCGCGAACTATCAAGCGATCATCCGTGa                    <  1:217326/150‑1 (MQ=255)
                                                                                                                                 gccgcgGCTCTGCAGCCCAAGGGTCACCAGCAGCTGCAGGCGGGCAAGGAATCGGTCAAGTCGAATATCGGCAGGGCGGTGGCGGCTACGGGCGCCGCTGCTGTGCCGACGATGAGCAGCGCGGACTATCAAGCGATCATCCGTGACAtt                >  2:599967/1‑149 (MQ=255)
                                                                                                                                         tCTGCAGCTCAAGGGTCACCAGCAGCTGCAGGCGGGCAAGGAATCGCTCAAGTCGAATATCGGCAGGGCGGTGGTGGCTACGGGCGCCGTTGCTGTGCCGACGATGGTCAGCGCGAACTATCAAGCGATCATCCGTGACATCGCGATCaa        <  1:234186/150‑1 (MQ=255)
                                                                                                                                               gCTCAAGGGTCACCAGCAGCTGCAGGCGGGCAAGGAATCGCTCAAGTCGAATATCGGCAGGGCGGTGGTGGCTACGGGCGCCGCTGCTGTGCCGACGATGATCAGCGCGAACTATCAAGCGATCATCCGTGACATCGCGATCAAGGCTGa  >  1:355945/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
GACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCACCAGCAGCTGCAGGCGGGCAAGGAATCGCTCAAGTCGAATATCGGCAGGGCGGTGGTGGCTACGGGCGCCGCTGCTGTGCCGACGATGATCAGCGCGAACTATCAAGCGATCATCCGTGACATCGCGATCAAGGCTGA  >  NC_002947/3441468‑3441760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑