Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,554,502 G→C 11.4% L25L (CTG→CTC)  PP_3139 → group 1 family glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,554,5020GC11.4% 126.4 / 7.5 44L25L (CTG→CTC) PP_3139group 1 family glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base G (18/21);  new base C (2/3);  total (20/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

GCCCGTAGTCGACAGCCTGAAGTGAAGGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGCTCAAGGGGTTGTTGGCACCGACGTGGCAGGT  >  NC_002947/3554354‑3554648
                                                                                                                                                    |                                                                                                                                                   
gCCCGTAGTCGACAGCCTGAAGTGAAGGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTTGCGCTgg                                                                                                                                                    >  1:464524/1‑150 (MQ=255)
           aCAGCCTGAAGTGAAGGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGCCGCGAAATACTGGCGCTCCAGCGCCagtat                                                                                                                                         <  1:157772/150‑5 (MQ=255)
                cTGAAGTGAAGGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGa                                                                                                                                    >  1:452481/1‑150 (MQ=255)
                 tgaagtgaagGTTGCCAGGGGAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAGAGATCTCGTCAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAg                                                                                                                                   <  1:61488/150‑1 (MQ=255)
                 tgaagtgaagGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAg                                                                                                                                   <  2:570496/150‑1 (MQ=255)
                           gTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCA‑ACGta                                                                                                                        >  2:211332/1‑150 (MQ=255)
                                 aGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGccc                                                                                                                  >  1:700132/1‑150 (MQ=255)
                                         ttGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTCCAGCGCCagta                                                                                                                                          <  2:317155/119‑4 (MQ=255)
                                         ttGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTCCAGCGCCagta                                                                                                                                          >  1:317155/1‑116 (MQ=255)
                                               atcaAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAAGCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGACGTGCA‑ACGTATAGCCCTGCGTGGGTGGGAc                                                                                                    <  1:473933/150‑1 (MQ=255)
                                                 caAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGATGGAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGACGc                                                                                                  >  1:61993/1‑150 (MQ=255)
                                                  aaGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGACGcc                                                                                                 <  1:757596/150‑1 (MQ=255)
                                                    gCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAg                                                                                                                                   >  2:714630/1‑115 (MQ=255)
                                                    gCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAg                                                                                                                                   <  1:714630/115‑1 (MQ=255)
                                                       ggTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAg                                                                                                                                           <  1:371455/104‑1 (MQ=255)
                                                       ggTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAg                                                                                                                                           >  2:371455/1‑104 (MQ=255)
                                                        gTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCA‑ACg                                                                                                                          <  2:25756/119‑1 (MQ=255)
                                                        gTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCA‑ACg                                                                                                                          >  1:25756/1‑119 (MQ=255)
                                                                    aTTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAg                                                                               <  2:464524/150‑1 (MQ=255)
                                                                                aTTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGac                                                                   >  2:832194/1‑149 (MQ=255)
                                                                                    cTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACggg                                                               <  2:845960/150‑1 (MQ=255)
                                                                                        cTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCaa                                                           >  1:254222/1‑150 (MQ=255)
                                                                                         ttCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAg                                                                               <  1:296475/129‑1 (MQ=255)
                                                                                         ttCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGGGGGACGCCGAAGTGCAGGATGCCGAg                                                                               >  2:296475/1‑129 (MQ=255)
                                                                                            atcaaacaGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGGTGAGGTGCA‑ACGTATCGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGAcc                                                       >  2:269215/1‑150 (MQ=255)
                                                                                             tcaatcaGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAGATCCTCGCGCTGGAACGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGACGCAAAAGTGCAGGATGCGGAGGACGCAAGCGAACGGGGCAAGAccc                                                      >  2:122398/1‑150 (MQ=255)
                                                                                             tcaatcaGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAg                                                                                                                                   <  1:481531/74‑1 (MQ=255)
                                                                                             tcaatcaGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAg                                                                                                                                   >  2:481531/1‑74 (MQ=255)
                                                                                               aatcaGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGc                                                    <  1:678100/150‑1 (MQ=255)
                                                                                                 tcaGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGACGCCGGATTTCAGGATGACGAGGACGCAAGCGAACGGGGCAAGACCCGCTa                                                  >  2:393912/1‑150 (MQ=255)
                                                                                                      aCCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTAt                                             <  2:700132/150‑1 (MQ=255)
                                                                                                       cccGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAGGCGATGGGGGCAAGACCCGCTACGTAtt                                            <  1:542885/150‑1 (MQ=255)
                                                                                                        ccGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCaa                                                           <  1:517512/134‑1 (MQ=255)
                                                                                                        ccGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCaa                                                           >  2:517512/1‑134 (MQ=255)
                                                                                                           aaaGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCGCGGGGGATTGTTTCAGGCGTGTGGCGGT‑CCTGGGTGGTGGGCGGTAGTGAAGGCTGAAGAGCACGAAATCGAACGGGGCAAGACCCGCTACGTATTGCaa                                        <  1:832194/150‑1 (MQ=255)
                                                                                                                 aGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTggg                                  <  2:254222/150‑1 (MQ=255)
                                                                                                                      caGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGCCGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTAGGTATTCCAATGTggagtcca                             >  2:370746/1‑144 (MQ=255)
                                                                                                                        gTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGCTCAAgg                           <  1:211332/150‑1 (MQ=255)
                                                                                                                            cATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGCTCAAGGGgtt                       <  1:103532/150‑1 (MQ=255)
                                                                                                                                           cttGGCGCTCCAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGa                                                                                      >  2:394666/3‑72 (MQ=255)
                                                                                                                                           cttGGCGCTCCAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGa                                                                                      <  1:394666/70‑1 (MQ=255)
                                                                                                                                              ggCGCTGGAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAg                                                                               >  1:5527/1‑76 (MQ=255)
                                                                                                                                              ggCGCTGGAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAg                                                                               <  2:5527/76‑1 (MQ=255)
                                                                                                                                                cgcTGGAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGCTCAAGGGGTTGTTGGCACCGACGTGGCAgg   <  1:370746/150‑1 (MQ=255)
                                                                                                                                                 gcTGGAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGCTCAAGGGGTTGTTGGCACCGACGTGGCAGGt  >  2:760863/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCCGTAGTCGACAGCCTGAAGTGAAGGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCA‑ACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGCTCAAGGGGTTGTTGGCACCGACGTGGCAGGT  >  NC_002947/3554354‑3554648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑