Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,684,036 C→A 12.9% L97M (CTG→ATG) ‡ PP_3250 → transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,684,0360CA12.9% 82.1 / 4.0 31L97M (CTG→ATG) ‡PP_3250transporter
Reads supporting (aligned to +/- strand):  ref base C (13/14);  new base A (2/2);  total (15/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01

TGGAAAACCTGCAGTGGCTGTTCACCGGCACCTTCATTGCCACGTTGGCGTGCCTGCCGCTGTTTGGCTGGCTGGCCTCCAGGGTGCAGCGCAGGCATATCCTGCCTTGGACTTATGGTTTTTTCGCCAGCAACCTGTTGCTGTTCGCGGCACTGTTGGCCGGTAACCCTGACGACCTCTGGACCGCCCGGGCGTTCTATATCTGGCTGTCGGTGTTCAATTTGCTGACGATCTCGCTGGCCTGGAGCGTGCTCGCCGATCTGTTTTCCACCGCCCAGG  >  NC_002947/3683902‑3684180
                                                                                                                                      |                                                                                                                                                
tGGAAAACCTGCAGTGGCTGTTCACCGGCACCTTCATTGCCACGTTGGCGTGCCTGCCGCTGTTTGGCTGGCTGGCCTCCAGGGTGCCACACAAGCATATCCTGCCTTGGACTTATGGTTTTTTCGCCAGCAACCTGTTGCTGTTCGCgg                                                                                                                                   >  2:117033/1‑150 (MQ=255)
         tGCAGTGGCTGTTCACCGGCACCTTCATTGCCACGTTGGCGTGCCTGCCGCTGTTTGGCTGGCTGGCCTCCAGGGTGCAGCGCAGGCATATCCTGCCTTGGACTTATGGTTTTTTCGCCAGCAACCTGTTGCTGTTCGCGGCACTGTTgg                                                                                                                          >  1:382755/1‑150 (MQ=255)
                  tGTTCACCGGCACCTTCATTGCCACGTTGGCGTGCCTGCCGCTGTTTGGCTGGCTGGCCTCCAGGGTGCAGCGCAGGCATATCCTGCCTTGGACTTATGGTTTTTTCGCCAGCAACCTGTTg                                                                                                                                             <  1:740686/122‑1 (MQ=255)
                  tGTTCACCGGCACCTTCATTGCCACGTTGGCGTGCCTGCCGCTGTTTGGCTGGCTGGCCTCCAGGGTGCAGCGCAGGCATATCCTGCCTTGGACTTATGGTTTTTTCGCCAGCAACCTGTTg                                                                                                                                             >  2:740686/1‑122 (MQ=255)
                  tGTTCACCGGCACCTTCATTGCCACGTTGGCGTGCCTGCCGCTGTTTGGCTGGCTGGCCTCCAGGGTGCAGCGCAGGCATATCCTGCCTTGGACTTATGGTTTTTTCGCCAGCAACCTGTTGCTGTTCGCGGCACTGTTGCCCgggcggc                                                                                                                 >  1:374131/1‑145 (MQ=255)
                      cACCGGCACCTTCATTGCCACGTTGGCGTGCCTGCCGCTGTTTGGCTGGCTGGCCTCCAGGGTGCAGCGCAGGCATATCCTGCCTTGGACTTATGGTTTTTTCGCCAGCAACCTGTTGCTGTTCGCGGCACTGTTGGCCGGTAACCCTga                                                                                                             <  2:170239/150‑1 (MQ=255)
                      cACCGGCACCTTCATTGCCACGTTGGCGTGCCTGCCGATGTTTGGCTGGCTGGCCTCCAGGGTGCAGCGCAGGCATATCCTGCCTTGGACTTATGGTTTTTTCGCCAGCAACCTGTTGCTGTTCGCGGCACTGTTGGCCGGTAACCCTga                                                                                                             <  1:545434/150‑1 (MQ=255)
                       cccGGCACCTTCATTGCCACGTTGGCGTGCCTGCCGCTGTTTGGCTGGCTGGCCTCCAGGGTGCAGCGCAGGCATATCCTGCCTTGGACTTATGGTTTTTTCGCCAGCAACCTGTTGCTGTTCGCGGCACTGTTGGCCGGTAACCCTgac                                                                                                            <  2:776585/149‑1 (MQ=255)
                               cTTCATTGCCACGTTGGCGTGCCTGCCGCTGTTTGGCTGGCTGGCCTCCAGGGTGCAGCGCAGGCATATCCTGCCTTGGACTTATGGTTTTTTCGCCAGCAACCTGTTGCTGTTCGCGGCACTGTTGGCCGGTAACCCTGACGACCTCTg                                                                                                    >  2:219118/1‑150 (MQ=255)
                               cTTCATTGCCACGTTGGCGTGCCTGCCGCTGTTTGGCTGGCTGGCCTCCAGGGTGCAGCGCAGGCATATCCTGCCTTGGACTTATGGTTTTTTCGCCAGCAACCTGTTGCTGTTCGCGGCACTGTTGGCCGGTAACCCTGACGACCTCTg                                                                                                    >  1:8991/1‑150 (MQ=255)
                                       ccACGTTGGCGTGCCTGCCGCTGTTTGGCTGGCTGGCCTCCAGGGTGCAGCGCAGGCATATCCTGCCTTGGACTTATGGTTTTTTCGCCAGCAACCTGTTGCTGTTCGCGGCACTGTTGGc                                                                                                                         >  1:572079/1‑121 (MQ=255)
                                       ccACGTTGGCGTGCCTGCCCCTGTTTGGCTGGCTGGCCTCCAGGGTGCAGCGCAGGCATATCCTGCCTTGGACTTATGGTTTTTTCGCCAGCAACCTGTTGCTGTTCGCGGCACTGTTGGc                                                                                                                         <  2:572079/121‑1 (MQ=255)
                                        cACGTTGGCGTGCCTGCCGCTGTTTGGCTGGCTGGCCTCCAGGGTGCAGCGCAGGCATATCCTGCCTTGGACTTATGGTTTTTTCGCCAGCAACAGGTTGCTggc                                                                                                                                        <  2:280170/105‑3 (MQ=255)
                                        cACGTTGGCGTGCCTGCCGCTGTTTGGCTGGCTGGCCTCCAGGGTGCAGCGCAGGCATATCCTGCCTTGGACTTATGGTTTTTTCGCCAGCAACAGGTTGCTggc                                                                                                                                        >  1:280170/1‑103 (MQ=255)
                                                        ccGCTGTTTGGCTGGCTGGCCTCCAGGGTGCAGCGCAGGCATATCCTGCCTTGGACTTATGGTTTTTTCGCCAGCAACCTGTTGCTGTTCGCGGCACTGTTGGCCGGTAACCCTGACGACCTCTGGACCGCCCGGGCGTTCTATATCTgg                                                                           <  2:359143/150‑1 (MQ=255)
                                                                    tggctggcCTCCAGGGTGCAGCGCAGGCATATCCTGCCTTGGACTTATGGTTTTTTCGCCAGCAACCTGTTGCTGTTCGCGGCACTGTTGGCCGGTAACCCTGACGACCTCTGGACCGCCCGGGCGTTCTATATCTGGCTGTCGGTGTTc                                                               >  2:767637/1‑150 (MQ=255)
                                                                        tggcCTCCAGGGTGCAGCGCAGGCATATCCTGCCTTGGACTTATGGTTTTTTCGCCAGCAACCTGTTGCTGTTCGCGGCACTGTTGGCCGGTAACCCTGACGACCTCTGGACCGCCCGGGCGTTCTATATCTGGCTGTCGGTGTTCAAtt                                                           >  2:813679/1‑150 (MQ=255)
                                                                                            aGGCATATCCTGCCTTGGACTTATGGTTTTTTCGCCAGCAACCTGTTGCTGTTCGCGGCACTGTTGGCCGGTAACCCTGACGACCTCTGGCCCGCCCGGGCGTTCTATATCTGCCTGTCGGTGTTCAATTTGCTGACGATCTCGCTGGcc                                       <  2:8991/150‑1 (MQ=255)
                                                                                                atatCCTGCCTTGGACTTATGGTTTTTTCGCCAGCAACCTGTTGCTGTTCGCGGCACTGTTGGCCGGTAACCCTGACGACCTCTGGACCGCCCGGGCGTTCTATATCTGGCTGTCGGTGTTc                                                               <  1:389615/122‑1 (MQ=255)
                                                                                                atatCCTGCCTTGGACTTATGGTTTTTTCGCCAGCAACCTGTTGCTGTTCGCGGCACTGTTGGCCGGTAACCCTGACGACCTCTGGACCGCCCGGGCGTTCTATATCTGGCTGTCGGTGTTc                                                               >  2:389615/1‑122 (MQ=255)
                                                                                                   tCCTGCCTTGGACTTATGGTTTTTTCGCCAGCAACCTGTTGCTGTTCGCGGCACTGTTGGCCGGTAACCCTGACGACCTCTGGACCGCCCGGGCGTTCTATATCTGGCTGTCGGTGTTCAATTTGCTGACGATCTCGCTGGCCTGGAGCg                                >  1:334905/1‑150 (MQ=255)
                                                                                                     cTGCCTTGGACTTATGGTTTTTTCGCCAGCAACCTGTTGCTGTTCGCGGCACTGTTGGCCGGTAACCCTgacgac                                                                                                         <  2:132154/75‑1 (MQ=255)
                                                                                                     cTGCCTTGGACTTATGGTTTTTTCGCCAGCAACCTGTTGCTGTTCGCGGCACTGTTGGCCGGTAACCCTgacgac                                                                                                         >  1:132154/1‑75 (MQ=255)
                                                                                                      tGCCTTGGACTTATGGTTTTTTCGCCAGCAACCTGTTGCTGTTCGCGGCACTGTTGGCCGGTAACCCTGACGACCTCTGGACCGCCCGGGCGTTCTATATCTGGCTGTCGGTGTTCAATTTGCTGACGATCTCGCTGGCCTGGAGCGTGc                             <  2:38056/150‑1 (MQ=255)
                                                                                                       gCCTTGGACTTATGGTCTTTTCGCCAGCAACCTGTTGCTGTTCGCGGCACTGTTGGCCGGTAACCTTGACGACCTCTGGACCGCCCGGGCGTTCTATATCTGGCTGTCGGTGTTCAATTTGCTGACGATCTCGCTGGCCTGGAGCgggtc                            <  2:79026/150‑5 (MQ=255)
                                                                                                          ttGGACTTATGGTTTTTTCGCCAGCAACCTGTTGCTGTTCGCGGCACTGTTGGCCGGTAACCCTGACGACCTCTGGACCGCCCGGGCGTTCTATATCTGGCTGTCGGTGTTCAATTTGCTGACGATCTCGCTGGCCTGGAGCGTGCTCGc                         <  1:767637/150‑1 (MQ=255)
                                                                                                                ttATGGTTTTTTCGACAGCAACCTGTTGCTGTTCGCGGCACTGTTGGCCGGTAACCCTGACGACCTCTGGACCGCCCGGGCGTTCTATATCTGGCTGTCGGTGTTCAATTTGCTGACGATCTCGCTGGCCTGGAGCGTGCTCGCCGATCt                   <  1:813679/150‑1 (MQ=255)
                                                                                                                       tttttCGCCAGCCACCTGTTGCTGTTCGCGGCACTGTCGGCCGGTATTCCGGACGACCTCTGGACCGCCCGGGCGTTCTATATCTGGCTGTCGGTGTTCAATTTGCTGACGATCGCTCTGGCCTGGATCGTGCTCGCCGATCTGTTTTcc            <  1:203491/150‑1 (MQ=255)
                                                                                                                              ccAGCAACCTGTTGCTGTTCGCGGCACTGTTGGCCGGTAACCCTGACGACCTCTGGACCGCCCGGGCGTTCTATATCTGGCTGTCGGTGTTCAATTTGCTGACGATCTCGCTGGCCTGGAGCGTGCTCGCCGATCTGTTTTCCACCGccc     >  1:271959/1‑150 (MQ=255)
                                                                                                                              acAGCAACAGGTTGCTGTTCGCGGCACTGTTGGCCGGTAACCCTGACGACCTCTGGACCGCCCGGGCGTTCTATATCTGGCTGTCGGTGTTCAATTTGCTGACGATCTCGCTGGCCTGGa                                   <  2:356421/119‑1 (MQ=255)
                                                                                                                              acAGCAACAGGTTGCTGTTCGCGGCACTGTTGGCCGGTAACCCTGACGACCTCTGGACCGCCCGGGCGTTCTATATCTGGCTGTCGGTGTTCAATTTGCTGACGATCTCGCTGGCCTGGa                                   >  1:356421/2‑120 (MQ=255)
                                                                                                                                 gcccccTGTTGTTATTCGCGGCACTGGTGACCGGTAACCCTGACGACCTCTGGACCGCCCGGGCGTTCTATATCTGGCTGTCGGTGTTCAATTTGCTGACGGGCTCGCTGGCCTGGAGCGTGCTCGCCGATCTGTTTTCCACCGCCCAgg  <  2:334905/146‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                                
TGGAAAACCTGCAGTGGCTGTTCACCGGCACCTTCATTGCCACGTTGGCGTGCCTGCCGCTGTTTGGCTGGCTGGCCTCCAGGGTGCAGCGCAGGCATATCCTGCCTTGGACTTATGGTTTTTTCGCCAGCAACCTGTTGCTGTTCGCGGCACTGTTGGCCGGTAACCCTGACGACCTCTGGACCGCCCGGGCGTTCTATATCTGGCTGTCGGTGTTCAATTTGCTGACGATCTCGCTGGCCTGGAGCGTGCTCGCCGATCTGTTTTCCACCGCCCAGG  >  NC_002947/3683902‑3684180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑