| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,711,943 | G→A | 13.2% | A27A (GCC→GCT) | paaB ← | ring 1,2‑phenylacetyl‑CoA epoxidase regulatory subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,711,943 | 0 | G | A | 13.2% | 87.6 / 6.2 | 38 | A27A (GCC→GCT) | paaB | ring 1,2‑phenylacetyl‑CoA epoxidase regulatory subunit |
| Reads supporting (aligned to +/- strand): ref base G (16/17); new base A (2/3); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01 | |||||||||||
CGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATCGCCGCTCAGGCGGCGTTCTTGTTCTGTTGC > NC_002947/3711801‑3712073 | cgtcCGAGGGGGCGAACAGCGGGGCGTGCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGGGGGTGTAGGGCTCGCGGGCGTTCGCGATGGCCATGGCGGCATCGGCGGCGTGCa < 2:183551/150‑1 (MQ=255) cGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCAGCGTGCACGcc > 2:272622/1‑149 (MQ=255) gAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGATCTCGCGGGCGGCCGCGACGTCCATGGCAGCATCGGCGGCGTgacagctc > 2:263564/1‑143 (MQ=255) gggCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGa < 1:283148/150‑1 (MQ=255) gCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCAGCGTGCACGCCGCCGatg < 1:272622/150‑3 (MQ=255) cGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGGTCTCGCTGGCGTTCTCGATGGCCATGGCGGCATCGGCAGCGTGCACGCCGCCGatgc < 2:543506/150‑4 (MQ=255) aaCAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGt < 2:34756/150‑1 (MQ=255) ggTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGt > 1:114057/1‑150 (MQ=255) gtcgGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGATCTCGGTGGTGTTCTCGTTGTTCATGACAACATAGGCGGCGTGCACACTGCCGACATGTTTGTGATTAAGGCCGtg > 2:399520/1‑150 (MQ=255) aggCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCCTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCa < 1:385567/150‑1 (MQ=255) gCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACg > 1:412376/1‑150 (MQ=255) ggTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGt > 2:571488/1‑101 (MQ=255) ggTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGt < 1:571488/101‑1 (MQ=255) ggTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGaa < 2:412376/150‑1 (MQ=255) tCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACtt < 1:252047/150‑1 (MQ=255) ccGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTCCTCGATGTCCATGGCGGGATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGGTTGCCGCTCACGAACACTTCGTaga > 2:835497/1‑150 (MQ=255) aaccaccCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCca < 2:121367/149‑1 (MQ=255) accaccCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCcag > 1:14764/1‑150 (MQ=255) acccacAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCcagaca > 1:342643/1‑150 (MQ=255) acccacAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCcagaca < 1:526373/150‑1 (MQ=255) tCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCTATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTtgtg > 2:230923/1‑91 (MQ=255) tCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCTATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTtgtg < 1:230923/91‑1 (MQ=255) cggcggTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATc < 1:477122/146‑1 (MQ=255) cTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATCGCCGCTCAggcgg > 1:627837/1‑150 (MQ=255) tCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTACGGCCGTGATTGTTGCGCACTAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATCGCCGCTCAggcggc < 1:104702/150‑1 (MQ=255) tCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGt > 2:539789/1‑73 (MQ=255) tCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGt < 1:539789/73‑1 (MQ=255) gTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGTGTCcaga > 2:845916/1‑101 (MQ=255) gTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGTGTCcaga < 1:845916/101‑1 (MQ=255) gTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATCGCCGCTCAggc > 2:509211/1‑139 (MQ=255) gTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATCGCCGCTCAggc < 1:509211/139‑1 (MQ=255) gTTCTCGATGGCCATGGAGGCATTGATGGCGTGCACGCGGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATCGCCGCTCAGGCGGCGTTCTTGt < 2:822189/150‑1 (MQ=255) tctcGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATCGCCGCTCAGGCGGCGTTCTTGTTc > 2:6844/1‑150 (MQ=255) tcGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATCGCCGCTCAGGCGGCGTTCTTGTTCTg > 1:554608/1‑150 (MQ=255) gATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATCGCCGCTCAGGCGGCGTTCTTGTTCTGtt > 2:784527/1‑150 (MQ=255) tGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATCGCCGCTCAGGCGGCGTTCTTGTTCTGttgc < 1:6844/150‑1 (MQ=255) tCGGCAGCGTGCACGCCGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCcaga > 2:179925/1‑79 (MQ=39) tCGGCAGCGTGCACGCCGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCcaga < 1:179925/79‑1 (MQ=255) | CGTCCGAGGGGGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATCGCCGCTCAGGCGGCGTTCTTGTTCTGTTGC > NC_002947/3711801‑3712073 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |