| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,731,892 | C→T | 11.6% | A464A (GCG→GCA) | PP_3299 ← | lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,731,892 | 0 | C | T | 11.6% | 108.0 / 5.6 | 43 | A464A (GCG→GCA) | PP_3299 | lipoprotein |
| Reads supporting (aligned to +/- strand): ref base C (18/20); new base T (3/2); total (21/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01 | |||||||||||
TACGCGGTATGAGCCCAATCCTCAACGAAGGTGAGTACGTCTTCTGCACCAAGGCATTTACGCAGTCCCATCATCGCTGTGCCACATCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGTCTATCAAACTGGCTACACCGTTCTGGAATGCTACGAAGGACGAACGTCGTGCTCGCTCCAGGGATACCTCACCGCGTT > NC_002947/3731750‑3732040 | tACGCGGTATGAGCCCAATCCTCAACGAAGGTGAGTACGTCTTCTGCACCAAGGCATTTACGCAGTCCCATCATCGCTGTGCCACATCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCGCGTgctgcta < 1:347560/150‑2 (MQ=255) cgGTATGAGCCCAATCCTCAACGAAGGTGAGTACGTCTTCTGCACCAAGGCATTTACGCAGTCCCATCATCGCTGTGCCACATCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGt < 2:664385/150‑1 (MQ=255) tCCTCAACGAAGGTGAGTACGTCTTCTGCACCAAGGCATTTACGCAGTCCCATCATCGCTGTGCCACATCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTc < 1:422917/150‑1 (MQ=255) tCAACGAAGGTGAGTACGTCTTCTGCACCAAGGCATTTACGCAGTCCCATCATCGCTGTGCCACATCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGa > 2:542939/1‑150 (MQ=255) aaCGAAGGTGAGTACGTCTTCTGCACCAAGGCATTTACGCAGTCCCATCATCGCTGTGCCACATCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGa‑aa > 1:38010/1‑150 (MQ=255) gAAGGTGAGTACGTCTTCTGCACCAAGGCATTTACGCAGTCCCATCATCGCTGTGCCACATCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACgcgc < 1:705912/119‑1 (MQ=255) gAAGGTGAGTACGTCTTCTGCACCAAGGCATTTACGCAGTCCCATCATCGCTGTGCCACATCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACgcgc > 2:705912/1‑119 (MQ=255) ggTGAGTACGTCTTCTGCACCAAGGCATTTACGCAGTCCCATCATCGCTGTGCCACATCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTc > 2:501660/1‑139 (MQ=255) ggTGAGTACGTCTTCTGCACCAAGGCATTTACGCAGTCCCATCATCGCTGTGCCACATCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTc < 1:501660/139‑1 (MQ=255) gTCTTCTGCACCAAGGCATTTACGCAGTCCCATCATCGCTGTGCCACATCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTgaga > 2:525706/1‑150 (MQ=255) tcttctGCACCAAGGCATTTACGCAGTCCCATCATCGCTGTGCCACATCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGaa < 1:525706/150‑1 (MQ=255) cAAGGCATTTACGCAGTCCCATCATCGCTGTGCCACATCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGAt > 1:824330/1‑130 (MQ=255) cAAGGCATTTACGCAGTCCCATCATCGCTGTGCCACATCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGAt < 2:824330/130‑1 (MQ=255) aaGGCATAAACGCAGTCCCACCATCGATATTCAAAATCTGAGTCAGTTGACCACCCGACACCCAGGGCGAGGTAGTCAGCAATAGCAGCACGCGCTGCTTCAGTTTGAGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCt < 2:158919/150‑1 (MQ=255) ggCATTTACGCAGTCCCATCATCGCTGTGCCACATCCGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCGTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGc < 1:107503/150‑1 (MQ=255) gCAGTCCCATCATCGCTGTGCCACATCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGGGTAGCTGCTGATCTGCGTTCAGAAc < 1:542939/150‑1 (MQ=255) gTCCCATCATCGCTGTGCCACATCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTAGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGTc < 1:452962/150‑1 (MQ=255) cccATCATCGCTGTGCCACATCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCCATAGCAGCACGCGCTGCTTCCGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGTCTa > 1:841697/1‑150 (MQ=255) atcGCTGTGCCACATCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCt < 2:87260/112‑1 (MQ=255) atcGCTGTGCCACATCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCt > 1:87260/1‑112 (MQ=255) atcGCTGTGCCACATCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGTCTATCAAAc > 1:848763/1‑150 (MQ=255) tcGCTGTGCCACATCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGTCTATCAAACt < 2:38010/150‑1 (MQ=255) ccACATCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGTCTATCAAACTGGCTacac > 2:648958/1‑150 (MQ=255) ccACATCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTAGCACCAACGGCCTTTCAG‑GAATGTGCTGATCTGGGTTCAGAACGTCTATCAAACTGGCTacac > 2:239850/1‑150 (MQ=255) cacaTCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGt < 2:516369/131‑1 (MQ=255) cacaTCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGt > 1:516369/1‑131 (MQ=255) aTCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGTCTATCAAACTGTCTACACCGtt > 1:498151/1‑150 (MQ=255) cTGAGTCAGTTGACCACCCGCCAGCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATATCCTAAGAAGCTGCGGATCTGCGTTCAGAACGTCTATCAAACTGGCTACACCGTTCt < 2:292395/150‑1 (MQ=255) cTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGTCTATCAAACTGGCTACACCGTTCt < 2:51075/150‑1 (MQ=255) ccaccCGCCACCCAGGGCGACGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGTCTATCAAACTGGCTACACCGTTCTGGAATGCTACGaa < 1:648958/150‑1 (MQ=255) ccGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGTCTATCAAACTGGCTACACCGTTCTGGAATGCTACGAAGGAc > 2:829157/1‑150 (MQ=255) cACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGTCTATCAAACTGGCTACACCGTTCTGGAATGCTACGAAGGACGAAc < 1:725156/150‑1 (MQ=255) aCCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGTCTATCAAACTGGCTACACCGTTCTGGAATGCTACGAAGGACGAAcg > 2:811089/1‑150 (MQ=255) cccAGGGCGAGGTAGGCAGCAATAGGCATGTTCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGTCTATCAAACTGGCTACACCGTTCTGGAATGCTACGAAGGACGAAcgt > 1:663291/1‑150 (MQ=255) gAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGTCTATCAAACTGGCTACACCGTTCTGGAATg < 2:15075/125‑1 (MQ=255) gAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGTCTATCAAACTGGCTACACCGTTCTGGAATg > 1:15075/1‑125 (MQ=255) ggCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGTCTATCAAACTGGCTACACCGTTCTGGAATGCTACGAAGGACGAACGTCGTGCTCGCTCCAg > 1:528174/1‑150 (MQ=255) actgaagcagcGCGTGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGTCTATCAAACTGGCTACACCGTTCTGGAATGCTACGAAGGACGAACGTCGTGCTCGCTCCAGGGAt > 1:412854/6‑150 (MQ=255) ctgaagcagcGCGTGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGTCTATCAAACTGGCTaca > 2:313412/5‑101 (MQ=255) ctgaagcagcGCGTGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGTCTATCAAACTGGCTaca < 1:313412/97‑1 (MQ=255) ctgaagcagcGCGTGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGTCTATCAAACTGGCTACACCGTTCTGGAATGCTACGAAGGACGAACGTCGTGCTCGCTCCAGGGATa > 1:197762/5‑150 (MQ=255) caATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGTCTATCAAACTGGCTACACCGTTCTGGAATGCTACGAAGGACGAACGTCGTGCTCGCTCCAGGGATa < 2:528174/150‑1 (MQ=255) aaTAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGTCTATCAAACTGTCTACACCGTTCTGGAATGCTACGAAGGACGAACGTCGTGCTCGCTCCAGGGATAc < 2:498151/150‑1 (MQ=255) aCGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGTCTATCAAACTGGCTACACCGTTCTGGAATGCTACGAAGGACGAACGTCGTGCTCGCTCCAGGGATAc > 2:319207/1‑141 (MQ=255) aCGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGTCTATCAAACTGGCTACACCGTTCTGGAATGCTACGAAGGACGAACGTCGTGCTCGCTCCAGGGATAc < 1:319207/141‑1 (MQ=255) gcgcTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTACACAGCTTCAGATCTTCTTTCAGAACGTCTATCAAACTGGCTACACCGTTCTGGAATGCTACGAAGGACGAACGTCGTGCTCGCTCCAGGGATACCTCACCGCGtt < 2:663291/150‑1 (MQ=255) | TACGCGGTATGAGCCCAATCCTCAACGAAGGTGAGTACGTCTTCTGCACCAAGGCATTTACGCAGTCCCATCATCGCTGTGCCACATCTGAGTCAGTTGACCACCCGCCACCCAGGGCGAGGTAGGCAGCAATAGCAGCACGCGCTGCTTCAGTTTGCGCAACTGCTCGGA‑AATCCGATGTCCTGAGAAGCTGCTGATCTGCGTTCAGAACGTCTATCAAACTGGCTACACCGTTCTGGAATGCTACGAAGGACGAACGTCGTGCTCGCTCCAGGGATACCTCACCGCGTT > NC_002947/3731750‑3732040 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |