| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,753,641 | A→C | 11.1% | V95V (GTT→GTG) | PP_3316 ← | chaperone‑associated ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,753,641 | 0 | A | C | 11.1% | 80.5 / 5.5 | 36 | V95V (GTT→GTG) | PP_3316 | chaperone‑associated ATPase |
| Reads supporting (aligned to +/- strand): ref base A (15/17); new base C (2/2); total (17/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.09e-01 | |||||||||||
TGGCGGCTTCCTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAGTGATCATCACACAACAG > NC_002947/3753502‑3753790 | tGGCGGCTTCCTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCg < 2:25822/150‑1 (MQ=255) ggcTTCCTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAg > 1:213820/1‑148 (MQ=255) ggcTTCCTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAg < 2:213820/148‑1 (MQ=255) gcTTCCTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTGCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGtct > 1:103977/1‑150 (MQ=255) cTTCCTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCGCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCTCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGtctt < 2:102230/150‑1 (MQ=255) tGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCg > 2:286433/1‑150 (MQ=255) aaCAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATg > 2:788183/1‑150 (MQ=255) cTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCaa > 1:687602/1‑150 (MQ=255) cTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGGTCCCCAGACGACGCTTAAGCCACTTCATCGGCATCCGTGGGAACGGATGCCGAGTCTTCTCCGATGCGCAGCaa > 1:687601/1‑150 (MQ=255) ttCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGcc > 2:654564/1‑150 (MQ=255) ttCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGcc > 1:504778/1‑150 (MQ=255) gCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCAt > 2:256169/1‑150 (MQ=255) gATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAAc < 2:852471/105‑1 (MQ=255) gATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAAc > 1:852471/1‑105 (MQ=255) aGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTTGCCACGGATGcc > 2:290283/1‑99 (MQ=255) aGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTTGCCACGGATGcc < 1:290283/99‑1 (MQ=255) cccGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTTGCCACGGATGc > 1:249131/1‑96 (MQ=255) cccGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTTGCCACGGATGc < 2:249131/96‑1 (MQ=255) tggcgtgCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAg > 2:443773/1‑150 (MQ=255) ggcgtgCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAACCAGCTGCTCATGAACTGTTCGAACAAACCGCTGCGCGAGCCGAAAAgg < 1:788183/150‑1 (MQ=255) gCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTc < 1:402839/150‑1 (MQ=255) cGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTgg < 2:272433/150‑1 (MQ=255) cGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTgg < 1:654564/150‑1 (MQ=255) tGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGa > 1:131397/1‑150 (MQ=255) cgcgGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGCCCGGTGCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGaaa < 1:581369/150‑1 (MQ=255) cAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGc > 2:802195/1‑150 (MQ=255) aGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTg < 2:103977/150‑1 (MQ=255) gacgCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGgcgc > 2:459210/1‑150 (MQ=255) ccATTTCATCGGCTTACGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAGCCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGc < 2:687601/150‑1 (MQ=255) ccACTTCATCGGCATGCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGc < 2:687602/150‑1 (MQ=255) cATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAGt < 2:504778/150‑1 (MQ=255) aTCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAg < 2:235325/114‑1 (MQ=255) aTCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAg > 1:235325/1‑114 (MQ=255) gCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAGTGatca < 2:343445/150‑1 (MQ=255) ccGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAGTGATCATcac < 1:459210/150‑1 (MQ=255) cAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAGTGATCATCACacaaca < 1:443773/150‑1 (MQ=255) aaCGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAGTGAtcatca < 2:112587/142‑1 (MQ=255) aaCGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAGTGAtcatca > 1:112587/1‑142 (MQ=255) aaCGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAGTGATCATCACACAACAg > 2:447025/1‑150 (MQ=255) | TGGCGGCTTCCTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAGTGATCATCACACAACAG > NC_002947/3753502‑3753790 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |