Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 243,623 T→A 11.8% K183I (AAA→ATA) ‡ fkl ← FKBP‑type peptidyl‑prolyl cis‑trans isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947243,6230TA11.8% 91.1 / 5.1 34K183I (AAA→ATA) ‡fklFKBP‑type peptidyl‑prolyl cis‑trans isomerase
Reads supporting (aligned to +/- strand):  ref base T (15/15);  new base A (2/2);  total (17/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01

AGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGTCTGGTTCTGGTCGAATATTGAGCCGTCCGGCAATCTGCCCACATAGCGCACTTGCACTTTGCCGC  >  NC_002947/243478‑243764
                                                                                                                                                 |                                                                                                                                             
agagGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGgc                                                                                                                                           <  2:837081/150‑1 (MQ=255)
                      ggTTGGCGACTGACGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAg                                                                                                                     >  1:116559/1‑150 (MQ=255)
                        ttGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCg                                                                                                                                       <  2:83445/130‑1 (MQ=255)
                        ttGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCg                                                                                                                                       >  1:83445/1‑130 (MQ=255)
                             gTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCt                                                                                                              >  1:11075/1‑150 (MQ=255)
                                    gCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCAAATGGCGcctg                                                                                                                                      >  1:278779/1‑117 (MQ=255)
                                    gCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCAAATGGCGcctg                                                                                                                                      <  2:278779/119‑3 (MQ=255)
                                      cAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCtt                                                                                                     >  2:338881/1‑150 (MQ=255)
                                                cagTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTc                                                                                                                           <  2:414829/118‑1 (MQ=255)
                                                cagTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTc                                                                                                                           >  1:414829/1‑118 (MQ=255)
                                                  gTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAAt                                                                                         >  1:98984/1‑150 (MQ=255)
                                                   gtCGGTCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATCGGCTTGCGCTGAAGGAATTACGAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCTAGCATTCGATAACCGACTc                                                                                        >  2:595010/2‑150 (MQ=255)
                                                        tctcGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCAAATGGCGcctg                                                                                                                                      <  1:364970/99‑3 (MQ=255)
                                                        tctcGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCAAATGGCGcctg                                                                                                                                      >  2:364970/1‑97 (MQ=255)
                                                            gAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGaa                                                                               <  1:338881/150‑1 (MQ=255)
                                                              aTACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAAcc                                                                                             >  1:681924/1‑134 (MQ=255)
                                                              aTACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAAcc                                                                                             <  2:681924/134‑1 (MQ=255)
                                                                    aGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTggg                                                                       <  2:98984/150‑1 (MQ=255)
                                                                        ggTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTACCGCTGCTGCACGTTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGt                                                                   >  1:574672/1‑150 (MQ=255)
                                                                          tgtAGGGGGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGGGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGtct                                                                 <  2:317686/150‑1 (MQ=255)
                                                                            tAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGtctgg                                                               <  1:720459/150‑1 (MQ=255)
                                                                              gggTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAgg                                                                                                                    <  2:292195/95‑1 (MQ=255)
                                                                              gggTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAgg                                                                                                                    >  1:292195/1‑95 (MQ=255)
                                                                                gTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGTGGGtcttgttct                                                           >  1:208939/1‑150 (MQ=255)
                                                                                    aaTCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGTCTGGTTCTGGTc                                                       >  2:15612/1‑150 (MQ=255)
                                                                                    aaTCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGTCTGGTTCTGGTc                                                       >  1:766090/1‑150 (MQ=255)
                                                                                                    cccGCGGCGCCATAGGCTTGCGCCGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGTCTGGTTCTGGTCGAATATTGAGCCGTcc                                       <  1:595010/150‑1 (MQ=255)
                                                                                                          gcgcCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGTCTGGTTCTGGTCGAATATTGAGCCGTCCGGCAAt                                 <  2:11075/150‑1 (MQ=255)
                                                                                                             ccATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGtctggttctg                                                          <  2:121908/122‑1 (MQ=255)
                                                                                                             ccATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGtctggttctg                                                          >  1:121908/1‑122 (MQ=255)
                                                                                                              cATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAAc                                                                                              <  2:435443/85‑1 (MQ=255)
                                                                                                              cATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAAc                                                                                              >  1:435443/1‑85 (MQ=255)
                                                                                                                                       cAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGTCTGGTTCTGGTCGAATATTGAGCCGTCCGGCAATCTGCCCACATAGCGCACTTGCACTTTgcc    <  2:766090/150‑1 (MQ=255)
                                                                                                                                         ggCGACATTTGGAGACGATGTAACTCTTCGTGAATGCGACTTGCCAGCCTTCGATAACCGATTCTATTTGTGTCCATTGGGGGGTCTGGTTCTGGTCGAATATTGAGCCGTCCGGCAATCTGCCCACATAGCGCACTTGCACTTTgccgc  <  1:15612/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
AGAGGGTGCAGTGCAGGCCTGAGGTTGGCGTCAGTCGCCAACCGCCAGCAGTTCGATCTCGAATACCAAGGGGGTGTAGGGTGCAATCAGGTCTCCCGCCCCCTCGGCGCCATAGGCTTGCGCTGAAGGAATTACCAGGCGCCATTTGGCGCCGGTGTGCATTTTCGGCAAGGCGACCTGCCAGCCTTCGATAACCGAATCCAGGTTGAACCATTGGGGGGTCTGGTTCTGGTCGAATATTGAGCCGTCCGGCAATCTGCCCACATAGCGCACTTGCACTTTGCCGC  >  NC_002947/243478‑243764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑