| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,763,545 | A→G | 16.2% | intergenic (+22/+1653) | PP_3326 → / ← PP_3328 | hypothetical protein/ring‑cleaving dioxygenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,763,545 | 0 | A | G | 16.2% | 64.2 / 6.4 | 31 | intergenic (+22/+1653) | PP_3326/PP_3328 | hypothetical protein/ring‑cleaving dioxygenase |
| Reads supporting (aligned to +/- strand): ref base A (15/11); new base G (3/2); total (18/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-01 | |||||||||||
CCGTACGCAATGAAGGACTCAACATCATCGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCTGCATGATGGGC > NC_002947/3763396‑3763686 | ccGTACGCAATGAAGGACTCAACATCATCGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCa > 2:200664/1‑150 (MQ=255) tACGCAATGAAGGACTCAACATCATCGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGa > 1:688546/1‑150 (MQ=255) tCAACATCATCGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGa < 1:301982/142‑1 (MQ=255) tCAACATCATCGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGa > 2:301982/1‑142 (MQ=255) ctcGCGGCACGGTCTACCTTTTCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAg < 2:688546/149‑1 (MQ=255) atcGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAg < 2:695902/150‑1 (MQ=255) gCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTc < 2:589658/150‑1 (MQ=255) tGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCt < 1:200664/150‑1 (MQ=255) aaTGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTACATCTCGGTCCTGGGGAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGGCATGATGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCaa > 1:662659/1‑150 (MQ=255) cccGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCAt < 2:734513/150‑1 (MQ=255) ttcaCTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCgg > 1:685921/1‑150 (MQ=255) aCTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCca > 2:40317/1‑150 (MQ=255) tCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCg > 1:540000/1‑85 (MQ=255) tCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCg < 2:540000/85‑1 (MQ=255) gTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGc < 2:685921/150‑1 (MQ=255) ttCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCa > 1:749867/1‑150 (MQ=255) ctagctAAGCAGCACCAGCAGTAAACTAAGCTACATGCCTCGTCCATGACGGCCGAGGTATGGAAGCGCGGGTCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATc < 1:53530/145‑1 (MQ=255) tGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCg > 1:499819/1‑150 (MQ=255) ggTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTg > 2:629872/1‑89 (MQ=255) ggTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTg < 1:629872/89‑1 (MQ=255) ggTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGaa < 1:335975/150‑1 (MQ=255) ccGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGAt > 1:768654/1‑150 (MQ=255) cacGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCCCACGGCAATCGAACACGATGAGCAt > 2:404569/1‑150 (MQ=255) cacGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCAt > 2:277255/1‑150 (MQ=255) gTAAACTAAGCGACATACCTCGGCCATGACGGCCGCGGTATGGAAGTTGGAGGCGTTCGGAATGATGGCCTGCAACCAGCATACGCACCATTACCATTGGCACAAGCCCGGCCGCGGCCACCCGGCACTCGAACACGATCGGCATGTCGa > 2:114299/1‑150 (MQ=255) aCTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTAc > 2:159547/1‑150 (MQ=255) tAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCt > 1:550764/1‑150 (MQ=255) tgccATACCTCGGCCGTCATGGCCGAGGTATGTCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGccc < 2:147589/96‑1 (MQ=37) tgccATACCTCGGCCGTCATGGCCGAGGTATGTCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGccc > 1:147589/4‑99 (MQ=37) cATACCTCGGCCGTCATGGCCGAGGTATGTCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCTGCatgat > 1:569294/1‑150 (MQ=255) aTACCTCGGCCGTCATGGCCGAGGTATGTCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCTGCatgatg < 2:569294/150‑1 (MQ=255) ccTCGGCCGTCATGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACGAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCTGAATGATgggg > 2:271597/1‑149 (MQ=255) | CCGTACGCAATGAAGGACTCAACATCATCGCGGCCCAGGCTACCCTGCCAATGGCAGACGGCAGCCCCGTGGCCCAGCAGAGCCTGTTCAGTTCACTGTCGTTCCTGGGTAAGCCGCACCACGAGTAAACTAAGCGACATACCTCGGCCATGACGGCCGAGGTATGGCAGTTGGAGGCGTTCGGAATGAGTGCCTGCAACCAGCATCCGCACCATGACCATTGGCACAAGCCCGGCTGCGGCCACACGGCAATCGAACACGATGGGCATGTCGATTACCTGCATGATGGGC > NC_002947/3763396‑3763686 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |