| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,766,195 | A→C | 12.2% | R161R (CGA→CGC) ‡ | PP_5599 → | LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,766,195 | 0 | A | C | 12.2% | 69.8 / 5.6 | 35 | R161R (CGA→CGC) ‡ | PP_5599 | LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (17/12); new base C (2/2); total (20/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.33e-01 | |||||||||||
TCAAGCATTGCCCCGCAACTGAGGCTACGCATCACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCCAGCAGTACTTGAATGACAATGACCTGCAGC > NC_002947/3766046‑3766343 | tCAAGCATTGCCCCGCAACTGAGGCTACGCATCACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCga < 2:606793/150‑1 (MQ=255)tCAAGCATTGCCCCGCAACTGAGGCTACGCATCACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCga < 1:821983/150‑1 (MQ=255) ccccGCAACTGAGGCTACGCATCACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCTCGAAGAAcctg < 2:794090/150‑3 (MQ=255) ccGCAACTGAGGCTACGCATCACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTg > 2:589862/1‑150 (MQ=255) ccGCAACTGAGGCTACGCATCACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTg > 2:550172/1‑150 (MQ=255) cGCAACTGAGGCTACGCATCACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGGGAAGAACCCCTGa > 1:91469/1‑150 (MQ=255) cGCAACTGAGGCTACGCATCACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGa > 2:283118/1‑150 (MQ=255) cGCATCACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCaaa < 1:223906/150‑1 (MQ=255) gCATCACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAg > 2:579207/1‑150 (MQ=255) ggggACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGaa < 1:550172/150‑2 (MQ=255) gggACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGc < 1:300195/126‑1 (MQ=255) gggACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGc > 2:300195/1‑126 (MQ=255) tCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAgcgc < 1:283118/150‑1 (MQ=255) tCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAAAACCCATGATGCTTTTGACCAAAGACCCCGTTGACAAgcgc > 2:139411/1‑150 (MQ=255) gTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATcc > 1:773718/1‑150 (MQ=255) gAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTcgacg > 1:596818/1‑150 (MQ=255) aCAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAGAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGAt > 1:737150/1‑150 (MQ=255) aaGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTc > 1:969/1‑150 (MQ=255) cTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTATTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCg > 1:122388/1‑150 (MQ=255) tGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGa > 2:616203/1‑150 (MQ=255) gggAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGa < 2:773718/150‑1 (MQ=255) gTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTc > 1:194848/1‑113 (MQ=255) gTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTc < 2:194848/113‑1 (MQ=255) tGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATg > 2:412939/1‑150 (MQ=255) gTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCt < 2:477647/112‑1 (MQ=255) gTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCt > 1:477647/1‑112 (MQ=255) aGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGAtt < 2:596818/150‑1 (MQ=255) gTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGcc < 1:579207/150‑1 (MQ=255) aaaaGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCcagcag > 1:719891/1‑150 (MQ=255) tCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCCAGCAGTACTTGaa < 1:589862/150‑1 (MQ=255) cGTATACAGGTTCTTCGGGAAGAACCCCTGATGCTTTTGATCAAAGGCCCCGTTGACAATCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCCAGCAGTACTTGaat < 2:91469/150‑1 (MQ=255) ggggTTCTTCTCGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCCAGCAGTACTTGaa > 1:715850/3‑143 (MQ=255) ggggTTCTTCTCGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCCAGCAGTACTTGaa < 2:715850/141‑1 (MQ=255) aGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAAACCACGTTGACAAGCACGACATCGGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCATGTGGTGGGAAGATTACCCAGAAGTATTTGaatgacaatg > 2:313739/1‑150 (MQ=255) ggTTCTTCTCGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCCAGCAGTACTTGaatgacaatga > 1:851236/1‑150 (MQ=255) ttcttcGAGAATAACCCCTGATGCTTTTGAACCCGGACCGCGAGGCGCAGCGCCGGAGCCGCTCGACGATTCTCGCTTTAAACCTGATGCGATATGACGCACATGCCTGGAGTGGGCAGATTGCCCAGCAGTACTTGAATGACAATGAcc < 1:616203/150‑1 (MQ=255) ttcttcGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCCAGCAGTACTTGAATGACAATGAcc < 1:272351/150‑1 (MQ=255) gagaAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGTGCAGATTGCCCAGCAGTACTTGAATGACAATGACCTGCatc > 1:311939/1‑148 (MQ=255) | TCAAGCATTGCCCCGCAACTGAGGCTACGCATCACGCCAGGGGACTCGCGCAGTCTTTACGAACAAGTAGCCTGCGGAGAGCTCGATGCGGGAGTCATGGTATGTCCGCCCTTCAGCGTCCCCAAAAGCATTCGTATACAGGTTCTTCGAGAAGAACCCCTGATGCTTTTGACCAAAGACCCCGTTGACAAGCGCGACATCCGCTCGACGATTCTTGGTCGAAACCTGATTCGATATGACTCACATGCCTGGGGTGGGCAGATTGCCCAGCAGTACTTGAATGACAATGACCTGCAGC > NC_002947/3766046‑3766343 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |