| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 269,112 | A→G | 10.5% | L281P (CTG→CCG) | desA ← | delta‑9 fatty acid desaturase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 269,112 | 0 | A | G | 10.5% | 92.9 / 3.3 | 38 | L281P (CTG→CCG) | desA | delta‑9 fatty acid desaturase |
| Reads supporting (aligned to +/- strand): ref base A (15/19); new base G (2/2); total (17/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.97e-01 | |||||||||||
GACTCGATGCGCACGTGGTGGCGGTCTTCCAGCAGGCTGGTTTCGCGCGAGAGCAGCCGTTTGGCACGGCGGAAGCGGTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTGGGCCATGATCTGGAAGCGGTTGTTGAGGATGGCCATGGCGGTGTCCATGTCCAGGCTGGCCTTGCCGGCCACCCGGTGGGCGATGGGCGCCACGCGCTGCACCTTGGCCAGACGCAGCAGGCAGAACAGGCGGATC > NC_002947/268977‑269261 | gACTCGATGCGCACGTGGTGGCGGTCTTCCAGCAGGCTGGTTTCGCGCGAGAGCAGCCGTTTGGCACGGCGGAAGCGGTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTgg > 1:503721/1‑150 (MQ=255) tGCGCACGTGGTGGCGGTCTTCCAGCAGGCTGGTTTCGCGCGAGAGCAGCCGTTTGGCGCGGCGGAAGCGGTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTGGGCCATGa < 2:829130/150‑1 (MQ=255) gcACGTGGTGGCGGTCTTCCAGCAGGCTGGTTTCGCGCGAGAGCAGCCGTTTGGCACGGCGGAAGCGGTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTGGGCCATGATCt < 1:36162/150‑1 (MQ=255) tggtggCGGTCTTCCAGCAGGCTGGTTTCGCGCGAGAGCAGCCGTTTGGCACGGCGGAAGCGGTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTGGGCCATGATCTGGAAg > 2:452965/1‑150 (MQ=255) ggtggCGGTCTTCCAGCAGGCTGGTTTCGCGCGAGAGCAGCCGTTTGGCACGGCGGAAGCGGTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTGGGCCATGATCTGGAAGc < 2:48247/150‑1 (MQ=255) cTTCCAGCAGGCTGGTTTCGCGCGAGAGCAGCCGTTTGGCACGGCGGAAGCGGTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGTGTGCGGTACTGGGCCATGATCTGGAAGCGGttgttgc > 1:262651/1‑149 (MQ=255) ccAGCAGGCTGGTTTCGCGCGAGAGCAGCCGTTTGGCACGGCGGAAGCGGTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTGGGCCATGATCTGGAAGCGGTTGTTGAGGa > 1:796767/1‑150 (MQ=255) agcagGCTGGTTTCGCGCGAGAGCAGCCGTTTGGCACGGCGGAAGCGGTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTgg < 2:52452/120‑1 (MQ=255) agcagGCTGGTTTCGCGCGAGAGCAGCCGTTTGGCACGGCGGAAGCGGTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTgg > 1:52452/1‑120 (MQ=255) agcagGCTGGTTTCGCGCGAGAGCAGCCGTTTGGCACGGCGGAAGCGGTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTGGGCCATGATCTGGAAGCGGTTGTTGAg > 2:311354/1‑146 (MQ=255) agcagGCTGGTTTCGCGCGAGAGCAGCCGTTTGGCACGGCGGAAGCGGTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCAGGCCGATCACCAGCTTGCGGTACTGGGCCATGATCTGGAAGCGGTTGTTGAg < 1:311354/146‑1 (MQ=255) tGGTTTCGCGCGAGAGCAGCCGTTTGGCACGGCGGAAGCGGTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCtt > 2:153586/1‑103 (MQ=255) tGGTTTCGCGCGAGAGCAGCCGTTTGGCACGGCGGAAGCGGTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCtt < 1:153586/103‑1 (MQ=255) tGGTTTCGCGCGAGAGCAGCCGTTTGGCACGGCGGAAGCGGTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCg < 2:796878/106‑1 (MQ=255) tGGTTTCGCGCGAGAGCAGCCGTTTGGCACGGCGGAAGCGGTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCg > 1:796878/1‑106 (MQ=255) ggTTTCGCGCGAGAGCAGCCGTTTGGCACGGCGGAAGCGGTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTGGGCCATGATCTGGAAGCGGTTGTTGAGGATGGCCATGGc < 2:579560/150‑1 (MQ=255) gcgAGAGCAGCCGTTTGGCACGGCGGAAGCGGTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTGGGCCATGATCTGGAAGCGGTTGTTGAGGATGGCCATGGCGGtgtcca > 1:33139/1‑150 (MQ=255) gcCGTTTGGCACGGCGGAAGCGGTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTGGGCCATGATCTGGAAGCGGTTGTTGAGGATGGCCGTGGCGGTGTCCATGTCCAGGc > 2:27995/1‑150 (MQ=255) cGTTTGGCACGGCGGAAGCGGTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTGGGCCATGATCTTGAAGCGGTTGTTGAGGATGGCCATGGCGGTGTCCATGTCCAGGCTg < 1:772998/150‑1 (MQ=255) cACGGCGGAAGCGGTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTGGGCCATGATCTGGAAGCGGTTGTTGAGGATGGCCATGGCGGTGTCCATGTCCAGGCTCGCCTTGc < 2:33139/150‑1 (MQ=255) cggcggAAGCGGTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTGGGCCATGATCTGGAAGCGGTTGTTGAGGATGGCCATGGCGGTGTCCATGTCCAGGCTGGCCTTGCCg < 1:471127/150‑1 (MQ=255) ggcggAAGCGGTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTGGGCCATGATCTGGAAGCGGTTGTTGAGGATGGCCATGGCGGTGTCCATGTCCAGGCTGGCCTTGCCgg > 2:828873/1‑150 (MQ=255) aaGCGGTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTGGGCCATGATCTGGAAGCGGTTGTTGAGGATGGCCATGGCGGTGTCCATGTCCAGGCTGGCCTTGCCGGCCAcc > 1:300667/1‑150 (MQ=255) gTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTGGGCCATGATCTGGAAGCGGTTGTTGAGGATGGCCATGGCGGTGTCCATGTCCAGGCTGGCCTTGCCGGCCACCCGGTg < 2:196795/150‑1 (MQ=255) tGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTGGGCCATGATCTGGAAGCGGTTGTTGAGGATGGCCATGGCGGTGTCCATGTCCAGGCTGGCCTTGCCGGCCACCCGGTgg < 2:631757/150‑1 (MQ=255) ccGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTGGGCCATGATCTGGAAGCGGTTGTTGAGGATGGCCATGGCGGTGTCCATGTCCAGGCTGGCCTTGCCGGCCACCCGGTGGGCGATgg < 2:796767/150‑1 (MQ=255) cGAGGCGTCGACACGGGCCAGTTCCTGCTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTGGGCCATGATCTGGAAGCGGTTGTTGAGGATGGCCATGGCGGTGTCCATGTCCAGGCTGGCCTTGCCGGCCACCCGGTGGGCGATggg < 1:817304/150‑1 (MQ=255) gCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTGGGCCATGATCTGGAAGCGGTTGTTGAGGATGGCCATGGCGGTGTCCATGTCCAGGCTGGCCTTGCCGGCCACCCGGTGGGCGATGGGCGcc > 1:131989/1‑150 (MQ=255) acGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTGGGCCATGATCTGGAAGCGGTTGTTGAGTATGGCCATGGCGGTGTCCATGTCCAGGCTGGCCTTGCCGGCCACCCGGTGGGCGATGGGCGCCACGCGCTg < 1:828873/150‑1 (MQ=255) gggCCAGTTCCTGTTTCACCAGCGGGCCGATCACCGGCTTGCGGTACTGGGCCATGATCTgg > 1:680207/1‑62 (MQ=255) gggCCAGTTCCTGTTTCACCAGCGGGCCGATCACCGGCTTGCGGTACTGGGCCATGATCTgg < 2:680207/62‑1 (MQ=255) gggCCAGTTCCTGTTTCACCAGCGGGCCGATCACCGGCTTGCGGTACTGGGCCATGATCTgg < 2:679437/62‑1 (MQ=255) gggCCAGTTCCTGTTTCACCAGCGGGCCGATCACCGGCTTGCGGTACTGGGCCATGATCTgg > 1:679437/1‑62 (MQ=255) aGTTCCTGTTTCGCCCGCGGGCCGATCACCAGCTTGCGGTACTGGGCCATGATCTGGAAGCGGTTGTTGAGGATGGCCATGGCGGTGTCCATGTCCAGGCTGGCCTTGCCGGCCACCCGGTGGGCGATGGGCGCCACGCGCTGCACCTTg < 2:262651/150‑1 (MQ=255) aGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTGGGCCATGATCTGGAAGCGGTTGTTGAGGATGGCCATGGCGGTGTCCATGTCCAGGCTGGCCTTGCCGGCCACCCGGTGGGCGATGGGCGCCACGCGCTGCACCTTg < 1:452965/150‑1 (MQ=255) gggCCGATCACCAGCTTGCGGTACTGGGCCATGATCTGGAAGCGGTTGTTGAGGATGGCCATGGCGGTGTCCATGTCCAGGCTGGCCTTGCCGGCCACCCGGTGGGCGATGGGCGCCACGCGCTGCACCTTGGCCAGACGCAgcaggcag > 2:204885/1‑150 (MQ=255) aTCACCAGCTTGCGGTACTGGGCCATGATCTGGAAGCGGTTGTTGAGGATGGCCATGGCGGTGTCCATGTCCAGGCTGGCCTTGCCGGCCACCCGGTGGGCGATGGGCGCCACGCGCTGCACCTTGGCCAGACGCAgcagg > 1:193713/1‑141 (MQ=255) aTCACCAGCTTGCGGTACTGGGCCATGATCTGGAAGCGGTTGTTGAGGATGGCCATGGCGGTGTCCATGTCCAGGCTGGCCTTGCCGGCCACCCGGTGGGCGATGGGCGCCACGCGCTGCACCTTGGCCAGACGCAgcagg < 2:193713/141‑1 (MQ=255) aGCTTGCGGTACTGGGCCATGATCTGGAAGCGGTTGTTGAGGATGGCCATGGCGGTGTCCATGTCCAGGCTGGCCTTGCCGGCCACCCGGTGGGCGATGGGCGCCACGCGCTGCACCTTGGCCAGACGCAGCAGGCAGAACAGGCGGATc > 2:567499/1‑150 (MQ=255) | GACTCGATGCGCACGTGGTGGCGGTCTTCCAGCAGGCTGGTTTCGCGCGAGAGCAGCCGTTTGGCACGGCGGAAGCGGTGGCGCACCGAGGCGTCGACACGGGCCAGTTCCTGTTTCACCAGCGGGCCGATCACCAGCTTGCGGTACTGGGCCATGATCTGGAAGCGGTTGTTGAGGATGGCCATGGCGGTGTCCATGTCCAGGCTGGCCTTGCCGGCCACCCGGTGGGCGATGGGCGCCACGCGCTGCACCTTGGCCAGACGCAGCAGGCAGAACAGGCGGATC > NC_002947/268977‑269261 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |