Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,782,006 T→A 16.2% F280I (TTC→ATC)  nikB → nickel ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,782,0060TA16.2% 84.2 / 11.8 37F280I (TTC→ATC) nikBnickel ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (17/14);  new base A (3/3);  total (20/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.82e-01

CTGGCTGCCGCTGGTGACGGCCACCGGCATGCACATCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGCATGAGTGTGCACGCTGCCCTGCGTCAGCCATTGCTGCTGCGCCGCCCC  >  NC_002947/3781858‑3782154
                                                                                                                                                    |                                                                                                                                                    
cTGGCTGCCGCTGGTGACGGCCACCGGCATGCACATCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCtt                                                                                                                                                     >  2:506492/1‑150 (MQ=255)
      gCCGCTGGTGACGGCCACCGGCATGCACATCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGAAGCACTGCATCAccgg                                                                                                                                               <  2:155955/150‑3 (MQ=255)
           tGGTGACGGCCACCGGCATGCACATCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCATTGCTTCACCCTGCTAc                                                                                                                                          >  1:200652/1‑150 (MQ=255)
                        cGGCATGCACATCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGAAGCACTGCATCAccg                                                                                                                                                <  2:181943/131‑2 (MQ=255)
                        cGGCATGCACATCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGAAGCACTGCATCAccg                                                                                                                                                >  1:181943/1‑130 (MQ=255)
                          gCATGCACATCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCtga                                                                                                                           <  1:41227/150‑1 (MQ=255)
                              gtttaTCCGCGAACTGCTCGGCGGCGCACTGGTGATCGAGCAGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTCCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTgatgat                                                                                                                       <  2:394178/146‑1 (MQ=255)
                                                 ggcggcGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGAt                                                                                                    <  1:77414/150‑1 (MQ=255)
                                                 ggcggcGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTAATGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGAt                                                                                                    >  1:852855/1‑150 (MQ=255)
                                                                aTCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTgg                                                                                     <  1:702267/150‑1 (MQ=255)
                                                                 tCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGc                                                                                    <  1:506492/150‑1 (MQ=255)
                                                                   gagaCGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGc                                                                                    >  1:703651/1‑148 (MQ=255)
                                                                   gagaCGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGc                                                                                    <  2:703651/148‑1 (MQ=255)
                                                                    agaCGTTCTTCGCCTGTCCCGGGGTTGGTCGTTTGGGGGTGTCGGCAGTGCTTAACGGCAACTTCCCGGTGATGCAGTGCTTCACCCTGCTGCTGACAGGCGTGCTGATGAAGTGTAAGGTGAGAGTCGATGTGTGCTACGCCTTGCCTg                                                                                 >  2:3170/1‑150 (MQ=255)
                                                                    agaCGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTg                                                                                 >  1:698375/1‑150 (MQ=255)
                                                                     gaCGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCATTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGa                                                                                <  2:200652/150‑1 (MQ=255)
                                                                     gaCGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGa                                                                                                     >  1:736094/1‑129 (MQ=255)
                                                                     gaCGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGa                                                                                                     <  2:736094/129‑1 (MQ=255)
                                                                            ttCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCCGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGc                                                                         >  2:400735/1‑150 (MQ=255)
                                                                                                tCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCct                                                                                                                                >  2:33842/1‑75 (MQ=255)
                                                                                                tCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCct                                                                                                                                <  1:33842/75‑1 (MQ=255)
                                                                                                tCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGaa                                                     >  2:582623/1‑150 (MQ=255)
                                                                                                    tttGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTGTAGCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGCa                                                 <  1:480456/150‑1 (MQ=255)
                                                                                                       gCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGGTCTGTAACCTGATAGTCGATGTGTGCTACGCCTGCGTTAAGCCGCGCAGCCGGCTGGAAGGGGTGAACGCATGa                                              <  1:627292/150‑1 (MQ=255)
                                                                                                                       ttAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGCATGAGTGTGCACGCTGCCCt                              >  1:62573/1‑150 (MQ=255)
                                                                                                                           ccGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGAtgtgtg                                                                                               <  1:672513/81‑1 (MQ=255)
                                                                                                                           ccGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGAtgtgtg                                                                                               >  2:672513/1‑81 (MQ=255)
                                                                                                                            cgcgACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGCATGAGTGTGCACGCTGCCCTGCGTc                         >  1:588386/1‑150 (MQ=255)
                                                                                                                            cgcgACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGCATGAGTGTGCACGCTGCCCTGCGTc                         >  2:787031/1‑150 (MQ=255)
                                                                                                                                gtagcagggTGAAGCACTGCATCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGCATGAGTGTGCACGCTGCCCTGCGTCAGcc                     >  2:77347/8‑150 (MQ=255)
                                                                                                                                 cTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATTTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGCATGAGTGTGCACGCTGCCCTGCGTCAGCCa                    >  2:379977/1‑150 (MQ=255)
                                                                                                                                  ttCCCGGTGATGCAGTGCTTCACCCTATCACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGCATGAGTGTGCACGCTGCCCTGCGTCAGCCAt                   >  1:22961/1‑150 (MQ=255)
                                                                                                                                   gcagggTGAAGCACTGCATCACCGTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAggg                                                          >  2:347988/5‑110 (MQ=255)
                                                                                                                                   gcagggTGAAGCACTGCATCACCGTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAggg                                                          <  1:347988/106‑1 (MQ=255)
                                                                                                                                       ggTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGCATGAGTGTGCACGCTGCCCTGCGTCAGCCATtgctg              >  1:585916/1‑150 (MQ=255)
                                                                                                                                        gTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCt                                                                                                                     <  1:202714/46‑1 (MQ=255)
                                                                                                                                        gTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCt                                                                                                                     >  2:202714/1‑46 (MQ=255)
                                                                                                                                                   cTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGCATGAGTGTGCACGCTGCCCTGCGTCAGCCATTGCTGCTGCGCCGccct  <  2:585916/150‑2 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTGGCTGCCGCTGGTGACGGCCACCGGCATGCACATCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGCATGAGTGTGCACGCTGCCCTGCGTCAGCCATTGCTGCTGCGCCGCCCC  >  NC_002947/3781858‑3782154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: