| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,798,747 | A→T | 13.4% | W66R (TGG→AGG) | PP_3358 ← | hydroxycinnamoyl‑CoA hydratase‑lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,798,747 | 0 | A | T | 13.4% | 100.0 / 8.0 | 38 | W66R (TGG→AGG) | PP_3358 | hydroxycinnamoyl‑CoA hydratase‑lyase |
| Reads supporting (aligned to +/- strand): ref base A (19/13); new base T (2/3); total (21/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
CCGTTGACCATGGCGATGGTCGGTTTGGCATACAGACGCAGCAACTTCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATGGCATTGCGTTTTTCCGGGCGATTGAGGGTCACCCAGGCGATGCCCG > NC_002947/3798599‑3798893 | ccGTTGACCATGGCGATGGTCGGTTTGGCATACAGACGCAGCAACTTCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAg < 2:501306/150‑1 (MQ=255) cGATGGTCGGTTTGGCATACAGACGCAGCAACTTCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCGAGTCCTGGAC‑CGCCGGCat < 2:194815/150‑2 (MQ=255) tGGTCGGTTTGGCATACAAACGCAGCAACTTCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATACCGGCG‑GTCCAGGACTCGCCGGCACCgg > 1:627428/1‑150 (MQ=255) tCGGTTTGGCATACAGACGCAGCAACTTCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCa > 1:569636/1‑150 (MQ=255) tttGGCATACAGACGCAGCAACTTCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCCGGACTCGCCGGCACCGGTCAAGAc < 2:53354/150‑1 (MQ=255) tGGCATACAGACGCATCAACTTCCCTTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGTAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCa < 2:159490/150‑1 (MQ=255) aTACAGACGCAGCAACTTCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCAc > 2:801546/1‑150 (MQ=255) cTTCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATAc > 1:145568/1‑129 (MQ=255) cTTCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATAc < 2:145568/129‑1 (MQ=255) cTTCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCagcgtcagcgtc > 2:72284/1‑150 (MQ=255) attgccattgcGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTGCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCAGCACCGGTCAATACCAGCACGCCGGCGTCAGCGTCCTGCt > 2:395211/1‑150 (MQ=255) ccattgcGAGGCTTCGCGGCGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCGAGTCCTGGAC‑CGCCGGCat > 1:1211/1‑109 (MQ=255) ccattgcGAGGCTTCGCGGCGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCGAGTCCTGGAC‑CGCCGGCat < 2:1211/110‑2 (MQ=255) attgcGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAgg < 2:840412/149‑1 (MQ=255) attgcGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAgg > 1:840412/1‑149 (MQ=255) aGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCa < 2:569636/150‑1 (MQ=255) ttttCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCGAGTCCTGGAC‑CGCCGGCat < 2:256710/85‑2 (MQ=37) ttttCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCGAGTCCTGGAC‑CGCCGGCat > 1:256710/1‑84 (MQ=37) tttCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTccc < 1:801546/150‑1 (MQ=255) tCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTgggg > 2:124785/1‑150 (MQ=255) cACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATGGCATTGCg > 2:777124/1‑150 (MQ=255) cTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATGGCATTGCGttt > 1:652394/1‑150 (MQ=255) gAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCCGCGTCCGCGGCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATGGCATTGCGTTTTTCCgg < 2:627428/150‑1 (MQ=255) gAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATGGCATTGCGTTTTTCCgg < 1:72284/150‑1 (MQ=255) aCTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGAc > 2:627547/1‑100 (MQ=255) aCTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGAc < 1:627547/100‑1 (MQ=255) cTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATGGCATTGCGTTTTTCCgg < 1:96901/141‑1 (MQ=255) cTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATGGCATTGCGTTTTTCCgg > 2:96901/1‑141 (MQ=255) cTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATGGCATTGCGTTTTTCCGGGCGATTGAg > 2:417927/1‑150 (MQ=255) cTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATGGCATTGCGTTTTTCCGGGCGATTGAg > 1:5890/1‑150 (MQ=255) gTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTc > 2:534020/1‑96 (MQ=255) gTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTc < 1:534020/96‑1 (MQ=255) tCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATGGCATTGCGTTTTTCCGGGCGATTGAGGGTCAcc > 1:72629/1‑150 (MQ=255) aTGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATGGCATTGCGTTTTTCCGGGCGATTGAg < 2:80121/140‑1 (MQ=255) aTGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATGGCATTGCGTTTTTCCGGGCGATTGAg > 1:80121/1‑140 (MQ=255) tGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATGGCATTGCGTTTTTCCGGGCGATTGAGGGTCACCCAgg > 2:383782/1‑150 (MQ=255) cggcg‑gTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATGGCATTGCGTTTTTCCGGGCGATTGAGGGTCACCCAGGCGa < 2:652394/150‑1 (MQ=255) tCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATGGCATAGCGTTTTTCCGGGCGATTGAGGGTCACCCAGGCGATGCCCg > 1:421446/1‑150 (MQ=255) | CCGTTGACCATGGCGATGGTCGGTTTGGCATACAGACGCAGCAACTTCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATGGCATTGCGTTTTTCCGGGCGATTGAGGGTCACCCAGGCGATGCCCG > NC_002947/3798599‑3798893 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |