| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 274,139 | T→G | 11.9% | K144T (AAG→ACG) | PP_0221 ← | methionine ABC transporter substrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 274,139 | 0 | T | G | 11.9% | 77.5 / 5.6 | 35 | K144T (AAG→ACG) | PP_0221 | methionine ABC transporter substrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (15/15); new base G (2/2); total (17/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.56e-01 | |||||||||||
CCTGCACCAGGTCGGCGTCGTCGTAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAACCCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGCACAAGGTT > NC_002947/273996‑274287 | ccTGCACCAGGTCGGCGTCGTCGTAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAACCCGGCCGTGGccc > 2:456438/1‑149 (MQ=255) cAGGTCGGCGTCGTCGTAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAACCCGGCCTTGGCcagcagcag < 1:804518/150‑1 (MQ=255) cAGGTCGGCGTCGTCGTAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAACCCGGCCTTGGCcagcagcag < 1:186701/150‑1 (MQ=255) gTCGGCGTCGTCGTAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATAAACCCTGCTCTGGCgagtagcagcag < 2:634901/150‑1 (MQ=255) cggcggcgtAGGCGCGTACCAGTGGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAACCCGGCCTTGGCCAGCAGCAGCAGGCCAc < 2:754123/144‑1 (MQ=255) gtcgtcgtAGGCGCGTACCAGTTGCTCCGCCTCGGCCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTCGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAACCCGGCCTTGGCCAGCAGCAGCAGGCCACg < 1:4359/150‑1 (MQ=255) gtAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAACCCGGCCGTGGCCTGCTGCTGCTGGCCAAGGCCggg < 1:340658/150‑3 (MQ=255) cAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAACCCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCggg < 2:162801/148‑1 (MQ=255) cAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAACCCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCggg > 1:162801/1‑148 (MQ=255) gCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAACCCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTgg > 1:185683/1‑150 (MQ=255) tCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAACCCGGCCTTGGCCAGCAGCAGCCGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAg < 1:517381/150‑1 (MQ=255) gCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAACCCGGCCTTGGCCAGCAGCAGCAGGCCACGGTCGCTGTTGATCGGGTCGTTGGCGTTGGCCACGCTGGCGCCTTCAGGCa < 2:346024/150‑1 (MQ=255) ccTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAACCCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCg > 1:76862/1‑150 (MQ=255) ccTTGTAGCCGACAACGGGGTGGGGGGTGATCAACCCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGc > 2:36141/1‑150 (MQ=255) tAGCCCACGCCGGGCTTGAGGGTGATCAACCCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACg > 2:448597/1‑150 (MQ=255) ccACGCCGGGCTTGAGGGTGATCAACCCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACgttgt > 1:489845/1‑150 (MQ=255) aCGCCGGGCTTGAGGGTGATCAACCCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGttgttg < 2:489845/150‑1 (MQ=255) cGGGCTTGAGGGTGATCAACCCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTtgatga > 1:1764/1‑150 (MQ=255) cGGGCTTGAGGGTGATCAACCCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTtgat < 2:428454/148‑1 (MQ=255) cGGGCTTGAGGGTGATCAACCCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTtgat > 1:428454/1‑148 (MQ=255) ttGAGGGTGATCAACCCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGgc > 2:607235/1‑83 (MQ=255) ttGAGGGTGATCAACCCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGgc < 1:607235/83‑1 (MQ=255) gggTGATCAACCCGGCCTTGGACAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGCGTACAGGCCAACGTTGTTGATGATGCCCgggg < 1:9563/150‑1 (MQ=255) ggTGATCAACCCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGc > 2:137048/1‑150 (MQ=255) tCAACCCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAg > 2:305324/1‑150 (MQ=255) tCAACCCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAg > 1:446751/1‑150 (MQ=255) cAACCAGCCCTTGGCCAGCAGCATCAGGACACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGGGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAgg < 2:369893/150‑1 (MQ=255) aacagcGGCCGTGGCCTGCTGCTGCTGGCCAAGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTtga > 1:155449/6‑131 (MQ=37) aacagcGGCCGTGGCCTGCTGCTGCTGGCCAAGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTtga < 2:155449/126‑1 (MQ=37) cccGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCa > 1:805867/1‑76 (MQ=255) cccGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCa < 2:805867/76‑1 (MQ=255) ccTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGCACAAGGt < 1:137048/150‑1 (MQ=255) cTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGt > 1:791273/1‑104 (MQ=255) cTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGt < 2:791273/104‑1 (MQ=255) cTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGCACAAGGtt > 2:167712/1‑150 (MQ=255) | CCTGCACCAGGTCGGCGTCGTCGTAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAACCCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGCACAAGGTT > NC_002947/273996‑274287 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |