Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,869,902 T→G 10.0% E354A (GAG→GCG) ‡ PP_3419 ← sigma‑54 dependent transcriptional regulator/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,869,9020TG10.0% 99.1 / 5.1 40E354A (GAG→GCG) ‡PP_3419sigma‑54 dependent transcriptional regulator/response regulator
Reads supporting (aligned to +/- strand):  ref base T (17/19);  new base G (2/2);  total (19/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01

CAACGGCTCGTCAACCAAGGTCATGCACAGGTTCAACTGATGCGCTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCTTCAGGGCGATAATGCGAAGGCGGAAGAACAGGTCACGCCTG  >  NC_002947/3869754‑3870051
                                                                                                                                                    |                                                                                                                                                     
cAACGGCTCGTCAACCAAGGTCATGCACAGGTTCAACTGATGCGCTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTtcggcctc                                                                                                                                                      >  2:591655/1‑150 (MQ=255)
        cGTCAACCAAGGTCATGCACAGGTTCAACTGATGCGCTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCGAGGCCGaaa                                                                                                                                              <  1:666934/150‑1 (MQ=255)
              ccAAGGTCATGCACAGGTTCAACTGATGCGCTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCa                                                                                                                                        <  1:110048/150‑1 (MQ=255)
              ccAAGGTCATGCACAGGTTCAACTGATGCGCTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCa                                                                                                                                        <  1:613672/150‑1 (MQ=255)
                          acaGGTTCAACTGATGCGCTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGCTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGc                                                                                                                            >  2:613844/1‑150 (MQ=255)
                                     tGATGCGCTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGc                                                                                                                            <  1:704758/139‑1 (MQ=255)
                                     tGATGCGCTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGc                                                                                                                            >  2:704758/1‑139 (MQ=255)
                                                  ccaccTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGTCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCaa                                                                                                    <  2:26595/150‑1 (MQ=255)
                                                   caccTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAg                                                                                                   >  2:177120/1‑150 (MQ=255)
                                                            cGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCcg                                                                                          <  1:767485/150‑1 (MQ=255)
                                                                   aGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGt                                                                                   <  1:177120/150‑1 (MQ=255)
                                                                        cacCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGaaa                                                                                               <  2:690311/133‑1 (MQ=255)
                                                                        cacCGTCTGCTCCAGCATGTTGCGCAATTCACGCACAGTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGaaa                                                                                               >  1:690311/1‑133 (MQ=255)
                                                                           cGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGAt                                                                           <  1:591655/150‑1 (MQ=255)
                                                                             tCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGt                                                                         >  2:548684/1‑150 (MQ=255)
                                                                                     aGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGc                                                                                                                            <  2:351642/91‑1 (MQ=255)
                                                                                     aGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGc                                                                                                                            >  1:351642/1‑91 (MQ=255)
                                                                                      gCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCAc                                                                >  1:416605/1‑150 (MQ=255)
                                                                                        aTGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCAcgc                                                              <  1:820522/150‑1 (MQ=255)
                                                                                              cgcAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATat                                                        <  2:586588/150‑2 (MQ=255)
                                                                                                  aTTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAAt                                                                                              >  1:370116/1‑108 (MQ=255)
                                                                                                  aTTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAAt                                                                                              <  2:370116/108‑1 (MQ=255)
                                                                                                  aTTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGgc                                                    >  1:332219/1‑150 (MQ=255)
                                                                                                   ttCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGACCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGgcg                                                   >  1:45086/1‑150 (MQ=255)
                                                                                                       cGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGc                                               <  1:369886/150‑1 (MQ=255)
                                                                                                        gCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGACTACAGGCGCGGCa                                              >  2:38113/1‑150 (MQ=255)
                                                                                                        gCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGgcgc                                                  <  2:610668/146‑1 (MQ=255)
                                                                                                        gCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGgcgc                                                  >  1:610668/1‑146 (MQ=255)
                                                                                                          acaTTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCAcc                                            >  2:746279/1‑150 (MQ=255)
                                                                                                             ttGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCTTc                                         <  1:831218/150‑1 (MQ=255)
                                                                                                              tGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCTTCa                                        <  2:332219/150‑1 (MQ=255)
                                                                                                               gccaggccagCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCcgcg                                                                                        >  1:292087/1‑101 (MQ=255)
                                                                                                               gccaggccagCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCcgcg                                                                                        <  2:292087/101‑1 (MQ=255)
                                                                                                                 caggccagCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCTTCAggg                                     <  2:156362/150‑1 (MQ=255)
                                                                                                                      cagCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTAtg                                                                                                            >  2:422219/1‑74 (MQ=255)
                                                                                                                      cagCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTAtg                                                                                                            <  1:422219/74‑1 (MQ=255)
                                                                                                                             aaCCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCTTCAGGGCGATAATGCGaa                         >  2:85521/1‑150 (MQ=255)
                                                                                                                                      gcagGCTTTCGGCCTAGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCATGGCCACGCGAATACATGCGCGGAACCTTCAGGGCGATAATGCGAAGGCGgtaga                >  2:235441/1‑150 (MQ=255)
                                                                                                                                         gcttttCGGCCGAGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCTTCAGGGCGATAATGCGAAGGCGGAAGAACa             >  1:478062/4‑150 (MQ=255)
                                                                                                                                           ttttCGGCCGAGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGc                                                                    >  1:789557/2‑93 (MQ=255)
                                                                                                                                           ttttCGGCCGAGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGc                                                                    <  2:789557/92‑1 (MQ=255)
                                                                                                                                                    tcggccGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCTTCAGGGCGATAATGCGAAGGCGGAAGAACAGGTCACGCCTg  >  2:217598/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAACGGCTCGTCAACCAAGGTCATGCACAGGTTCAACTGATGCGCTTGCACCACCTCGTTCGGCGCCAGCAACACCGTCTGCTCCAGCATGTTGCGCAATTCACGCACATTGCCAGGCCAGCTATAACCGAGCAGCAGGCTTTCGGCCTCGGCCGAAAAGCGCAGGTTCGGCTTGCCATAGCGCCGGCTATGGTGGGCAAGGAAATGCCGCGCCAGTAGCAGGATGTCCTGCCCACGCGAATACAGGCGCGGCACCTTCAGGGCGATAATGCGAAGGCGGAAGAACAGGTCACGCCTG  >  NC_002947/3869754‑3870051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑