Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,876,231 C→G 9.5% L227V (CTG→GTG)  xcpS → type II secretion pathway protein XcpS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,876,2310CG 9.5% 127.3 / 5.4 42L227V (CTG→GTG) xcpStype II secretion pathway protein XcpS
Reads supporting (aligned to +/- strand):  ref base C (22/16);  new base G (2/2);  total (24/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

CTGGTGGGAGGCGGTGTGGTGCTGTTCCTGCTCGGCTATGTGGTGCCGCGTTTCAGCCAGGTGTTCGAAGGCATGGGCACCGAGCTACCCTGGCTGTCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTGGGCCTCCTGCCAGCTGCGGCGCCTGCCCGCACTGCACCAGCGCCTGATGATGTACGAGCTGGCGCGTTTCTATCGC  >  NC_002947/3876084‑3876377
                                                                                                                                                   |                                                                                                                                                  
cTGGTGGGAGGCGGTGTGGTGCTGTTCCTGCTCGGCTATGTGGTGCCGCGTTTCAGCCAGGTGTTCGAAGGCATGGGCACCGAGCTACCCTGGCTGTCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTg                                                                                                                                                  >  1:387084/1‑150 (MQ=255)
     gggAGGCGGTGTGGTGCTGTTCCTGCTCGGCTATGTGGTGCCGCGTTTCAGCCAGGTGTTCGAAGGCATGGGCACCGAGCTACCCTGGCTGTCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCt                                                                                                                                             <  2:387084/150‑1 (MQ=255)
       gAGGCGGTGTGGTGCTGTTCCTGCTCGGCTATGTGGTGCCGCGTTTCAGCCAGGTGTTCGAAGGCATGGGCACCGAGCTACCCTGGCTGTCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCAGTGGCGCttg                                                                                                                                           <  2:628395/150‑3 (MQ=255)
                  gtgCTGTTCCTGCTCGGCTATGTGGTGCCGCGTTTCAGCCAGGTGTTCGAAGGCATGGGCACCGAGCTACCCTGGCTGTCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGt                                                                                                                                >  1:17031/1‑150 (MQ=255)
                   tgCTGTTCCTGCTCGGCTATGTGGTGCCGCGTTTCAGCCAGGTGTTCGAAGGCATGGGCACCGAGCTACCCTGGCTGTCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTgg                                                                                                                                           >  1:299743/1‑138 (MQ=255)
                   tgCTGTTCCTGCTCGGCTATGTGGTGCCGCGTTTCAGCCAGGTGTTCGAAGGCATGGGCACCGAGCTACCCTGGCTGTCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTgg                                                                                                                                           <  2:299743/138‑1 (MQ=255)
                                               gcgTTTCAGCCAGGTGTTCGAAGGCATGGGCACCGAGCTACCCTGGCTGTCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCtgctgc                                                                                                       >  2:45438/1‑146 (MQ=255)
                                               gcgTTTCAGCCAGGTGTTCGAAGGCATGGGCACCGAGCTACCCTGGCTGTCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCtgctgc                                                                                                       <  1:45438/146‑1 (MQ=255)
                                                     cAGCCAGGTGTTCGAAGGCATGGGCACCGAGCTACCCTGGCTGTCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCAGTGGCGCttg                                                                                                                                           >  2:586799/1‑102 (MQ=255)
                                                     cAGCCAGGTGTTCGAAGGCATGGGCACCGAGCTACCCTGGCTGTCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCAGTGGCGCttg                                                                                                                                           <  1:586799/104‑3 (MQ=255)
                                                        ccAGGTGTTCGAAGACATGGGCACCGAGCTACCCTGGCTGTCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCcgcg                                                                                          >  1:332985/1‑150 (MQ=255)
                                                          aGGTGTTCGAAGGCATGGGCACCGAGCTACCCTGGCTGTCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTg                                                                                                                               <  1:526507/111‑1 (MQ=255)
                                                          aGGTGTTCGAAGGCATGGGCACCGAGCTACCCTGGCTGTCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTg                                                                                                                               >  2:526507/1‑111 (MQ=255)
                                                               ttCGAAGGCATGGGCACCGAGCTACCCTGGCTGTCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGCCCCCTCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACgg                                                                                   >  1:437807/1‑150 (MQ=255)
                                                                  gAAGGCATGGGCACCGAGCTACCCTGGCTGTCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTAc                                                                                >  2:831556/1‑150 (MQ=255)
                                                                    aGGCATGGGCACCGAGCTACCCTGGCTGTCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGGCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTg                                                                              <  1:831556/150‑1 (MQ=255)
                                                                      gCATGGGCACCGAGCTACCCTGGCTGTCACGGGTGCTGATGCAGGTCGGCCGGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTggg                                                                            >  1:384212/1‑150 (MQ=255)
                                                                         tGGGCACCGAGCTACCCTGGCTGTCACGGGTACTGATGCAGGTAGGACTGTTTCTGCATGACCAGCAAGCGCCACTGGCGCTGTGTATTTTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTGGGcct                                                                         >  2:524377/1‑150 (MQ=255)
                                                                          gggCACCGAGCTACCCTGGCTGTCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTGGGcctc                                                                        >  1:539260/1‑150 (MQ=255)
                                                                                      aCCCTGGCTGTCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTGGGCCTCCTGCCAGCTgcg                                                            >  1:171571/1‑150 (MQ=255)
                                                                                       cccTGGCTGTCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTgg                                                                             >  2:142789/1‑132 (MQ=255)
                                                                                       cccTGGCTGTCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTgg                                                                             <  1:142789/132‑1 (MQ=255)
                                                                                       cccTGGCTGTCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTGGGCCTCCTGCCAGCTgcgg                                                           >  2:310893/1‑150 (MQ=255)
                                                                                             cTGTCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTGGGCCTCCTGCCAGCTGCGGCGCCTg                                                     <  2:318697/150‑1 (MQ=255)
                                                                                             cTGTCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTGGGCCTCCTGCCAGCTGCGGCGCCTg                                                     <  1:778548/150‑1 (MQ=255)
                                                                                                tCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGgc                                                                                                             <  2:795757/91‑1 (MQ=255)
                                                                                                tCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGgc                                                                                                             >  1:795757/1‑91 (MQ=255)
                                                                                                    gggTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTGGGCCTCCTGCCAGCTGCGGCGCCTGCCCg                                                 <  2:20986/147‑1 (MQ=255)
                                                                                                    gggTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTGGGCCTCCTGCCAGCTGCGGCGCCTGCCCg                                                 >  1:20986/1‑147 (MQ=255)
                                                                                                       tGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTGGGCCTCCTGCCAGCTGCGGCGCCTGCCCGCACTGc                                           >  2:511383/1‑150 (MQ=255)
                                                                                                             tGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTGGGCCTCCTGCCAGCTGCGGCGCCTGCCCGCACTGCACCAgc                                     <  2:171571/150‑1 (MQ=255)
                                                                                                                   tCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTGGGCCTCCTGCCAGCTGCGGCGCCTGCCCGCACTGCACCAGCGCCtga                               <  1:48924/150‑1 (MQ=255)
                                                                                                                     ggCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTGGGCCTCCTGCCAGCTGCGGCGCCTGCCCGCCCTGCACCAGCGCCtgatg                             >  2:834236/1‑150 (MQ=255)
                                                                                                                       ccTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTGGGCCTCCTGCCAGCTGCGGCGCCTGCCCGCACTGCACCAGCGCCTgatgat                           >  2:767053/1‑150 (MQ=255)
                                                                                                                           tttCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTGGGCCTCCTGCCAGCTGCGGCGCCTGCCCGCACTGCACCAGCGCCTGATGATGTAc                       <  2:646644/150‑1 (MQ=255)
                                                                                                                           tttCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTGGGCCTCCTGCCAGCTGCGGCGCCTGCCCGCACTGCACCAGCGCCTGATGATGTAc                       >  1:396049/1‑150 (MQ=255)
                                                                                                                                gCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTGGGCCTCCTGCCAGCTGCGGCGCCTGCCCGCACTGCACCAGCGCCTGATGATGTACGAGCt                  >  1:277034/1‑150 (MQ=255)
                                                                                                                                       tgcccaGCGCCAGTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTGGGCCTCCTGCCAGCTGCGGCGCCTGCCCGCACTGCACCAGCGCCTGATGATGTACGAGCTGGCGCGt           >  2:160386/6‑150 (MQ=255)
                                                                                                                                       cAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTGGGCCTCCTGCCAGCTGCGGCGCCTGCCCGCACTGCACCAGCGCCTGATGATGTACGAGCTGGCGCGt           <  2:17031/150‑1 (MQ=255)
                                                                                                                                        aGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTGGGCCTCCTGCCAGCTGCGGCGCCTGCCCGCACTGCACCAGCGCCTGATGATGTACGAGCTGGCGCGtt          <  2:332985/150‑1 (MQ=255)
                                                                                                                                         gCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTGGGCCTCCTGCCAGCTGCGGCGCCTGCCCGCACTGCACCAGCGCCTGATGATGTACGAGCTGGCGCGttt         <  2:384212/150‑1 (MQ=255)
                                                                                                                                                ccACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTGGGCCTCCTGCCAGCTGCGGCGCCTGCCCGCACTGCACCAGCGCCTGATGATGTACGAGCTGGCGCGTTTCTAtcgc  >  2:290071/1‑150 (MQ=255)
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CTGGTGGGAGGCGGTGTGGTGCTGTTCCTGCTCGGCTATGTGGTGCCGCGTTTCAGCCAGGTGTTCGAAGGCATGGGCACCGAGCTACCCTGGCTGTCACGGGTGCTGATGCAGGTCGGCCTGTTTCTGCATGCCCAGCAAGCGCCACTGGCGCTGGGCACCGTCGGTGGGGTCGCGGCGCTTTGGCTGCTGCGCCGGCACCCGCGTGTACGGTACTGGGCCTCCTGCCAGCTGCGGCGCCTGCCCGCACTGCACCAGCGCCTGATGATGTACGAGCTGGCGCGTTTCTATCGC  >  NC_002947/3876084‑3876377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑