Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,908,667 A→G 11.1% D191G (GAC→GGC)  PP_3450 → TPR repeat‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,908,6670AG11.1% 137.5 / 7.9 54D191G (GAC→GGC) PP_3450TPR repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (22/26);  new base G (3/3);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01

CCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGCCCCCGCAGCCGCGCACCGCCGCAGAATGGGAACAACGCTATCGGCAGACCGGC  >  NC_002947/3908519‑3908815
                                                                                                                                                    |                                                                                                                                                    
ccTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGAc                                                                                                                                                     <  2:319554/150‑1 (MQ=255)
   gATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTg                                                                                                                                                  >  1:11421/1‑150 (MQ=255)
    aTCAACTGCAGCAACCCGAGCAGCGCCCGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTgg                                                                                                                                                 <  2:399412/150‑1 (MQ=255)
       aaCTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGccc                                                                                                                                              <  1:68227/150‑1 (MQ=255)
          tGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCggg                                                                                                                                           >  2:246815/1‑150 (MQ=255)
           gcagcaACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGa                                                                                                                                          <  2:204640/150‑1 (MQ=255)
              gcaACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACa                                                                                                                                       >  1:518553/1‑150 (MQ=255)
                aaCCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGt                                                                                                                                     >  1:359040/1‑150 (MQ=255)
                  cccGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTc                                                                                                                                   <  1:245874/150‑1 (MQ=255)
                    cGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGTAACAGTTCaa                                                                                                                                 <  2:463626/150‑1 (MQ=255)
                      agcagcGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAgg                                                                                                                               >  1:200109/1‑150 (MQ=255)
                           cgcCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGGCCTGGCCCGGGAACAGTTCAAGGCCTGc                                                                                                                          <  1:717140/150‑1 (MQ=255)
                           cgcCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGc                                                                                                                          <  2:200109/150‑1 (MQ=255)
                                gCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGc                                                                                                                     <  1:460600/150‑1 (MQ=255)
                                 cAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCg                                                                                                                                             <  2:501590/125‑1 (MQ=255)
                                 cAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCg                                                                                                                                             >  1:501590/1‑125 (MQ=255)
                                       tGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGGCCAGGTCCgggtc                                                                                                                                         <  2:518094/123‑3 (MQ=255)
                                       tGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGGCCAGGTCCgggtc                                                                                                                                         >  1:518094/1‑121 (MQ=255)
                                           aaCTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGAcc                                                                                                          <  1:246815/150‑1 (MQ=255)
                                                            gacgaTGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGGCCCTCCAACGCCGACCCTCAGGCCTATGCCGTg                                                                                         >  2:223133/1‑150 (MQ=255)
                                                                     gcgcTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGAACCTCAGGCCTATGCCGTGCTCAagcag                                                                                <  2:11421/150‑1 (MQ=255)
                                                                         tGGTGCGGCGCCTGGGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCACCGCCGACCCTCAGGCCTAGGCCGTGCTCAAGCAGCGcc                                                                            <  2:98671/150‑1 (MQ=255)
                                                                           gTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAATGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGcc                                                                            >  2:620206/1‑148 (MQ=255)
                                                                           gTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGcc                                                                            <  1:620206/148‑1 (MQ=255)
                                                                              cggcgCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTAt                                                                       <  1:588826/150‑1 (MQ=255)
                                                                               ggcgCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATg                                                                      <  1:40898/150‑1 (MQ=255)
                                                                                    cTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGcc                                                                 <  1:394916/150‑1 (MQ=255)
                                                                                     tGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATgg                                                                     >  1:672154/1‑145 (MQ=255)
                                                                                     tGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATgg                                                                     <  2:672154/145‑1 (MQ=255)
                                                                                         cAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGGCCAGGTCCgggtct                                                                                                                                        >  2:666861/1‑71 (MQ=38)
                                                                                         cAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGGCCAGGTCCgggtct                                                                                                                                        <  1:666861/74‑4 (MQ=38)
                                                                                          aaCGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGaa                                                                                                                                         <  1:168207/72‑1 (MQ=255)
                                                                                          aaCGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGaa                                                                                                                                         >  2:168207/1‑72 (MQ=255)
                                                                                                cTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACTCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGccc                                                     <  2:311109/150‑1 (MQ=255)
                                                                                                     gcgcCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGc                                                       >  1:672387/1‑143 (MQ=255)
                                                                                                     gcgcCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGc                                                       <  2:672387/143‑1 (MQ=255)
                                                                                                              ccTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGCCCCCGCAGCCGCGCAc                                       >  1:707246/1‑150 (MQ=255)
                                                                                                                  gAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGCCCCCGCAGCCGCGCAccgcc                                   >  1:622110/1‑150 (MQ=255)
                                                                                                                             cTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCATATTAATTGCCCATCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGCCCCCGCAGCCGCGCACCGCCGCAGAATGGGa                        <  2:331557/150‑1 (MQ=255)
                                                                                                                              tGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGCCCCCGCAGCCGCGCACCGCCGCAGAATGGGaa                       <  2:555963/150‑1 (MQ=255)
                                                                                                                                  tGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGCCCCCGCAGCCGCGCACCGCCGCAGAATGGGaacaac                   >  1:757417/1‑150 (MQ=255)
                                                                                                                                        aTCACGCCCCGGACCTGGCCTGGGCACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGTCCCCACAGCCGCGCACCGCCGCAGAATGGGAACAACGCTATc             <  2:518553/150‑1 (MQ=255)
                                                                                                                                        aTCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAagc                                                                                  >  1:207433/1‑81 (MQ=255)
                                                                                                                                        aTCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAagc                                                                                  <  2:207433/81‑1 (MQ=255)
                                                                                                                                           tgttcccGGGCCAGGTCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGCCCCCGCAGCCGCGCACCGCCGCAGAATGGGAACAACGCTATCGGc          >  2:322846/5‑150 (MQ=255)
                                                                                                                                               cccGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAagcagc                                                                               <  1:693635/77‑1 (MQ=255)
                                                                                                                                               cccGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAagcagc                                                                               >  2:693635/1‑77 (MQ=255)
                                                                                                                                               cccGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGACCCGGCAGCCGCGCACCGCCGCAGAATGGGAACAACGCTATGGGCGGag      >  1:388833/1‑149 (MQ=255)
                                                                                                                                                ccGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTAt                                                                       <  1:381251/84‑1 (MQ=255)
                                                                                                                                                ccGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTAt                                                                       >  2:381251/1‑84 (MQ=255)
                                                                                                                                                ccGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGCCCCCGCAGCCGCGCACCGCCGCAGAATGGGAACAACGCTATCGGCAGAcc     >  1:680747/1‑150 (MQ=255)
                                                                                                                                                ccGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGCCCCCGCAGCCGCGCACCGCCGCAGAATGGGAACAACGAGATCGGAAGagc     >  1:331920/1‑148 (MQ=255)
                                                                                                                                                 cGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGCCCCCGCAGCCGCGCAGCGCCGCAGAATGGGAACAACGCTATCGGCAGACCg    >  2:458648/1‑150 (MQ=255)
                                                                                                                                                   gACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGCCCCCGCAGCCGCGCACCGCCGCAGAATGGGAACAACGCTATCGGCAGACCGGc  >  1:488902/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGCCCCCGCAGCCGCGCACCGCCGCAGAATGGGAACAACGCTATCGGCAGACCGGC  >  NC_002947/3908519‑3908815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑