Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,908,670 T→A 9.4% L192Q (CTG→CAG)  PP_3450 → TPR repeat‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,908,6700TA 9.4% 155.7 / 5.3 53L192Q (CTG→CAG) PP_3450TPR repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (22/26);  new base A (3/2);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

GATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGCCCCCGCAGCCGCGCACCGCCGCAGAATGGGAACAACGCTATCGGCAGACCGGC  >  NC_002947/3908522‑3908815
                                                                                                                                                    |                                                                                                                                                 
gATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTg                                                                                                                                                  >  1:11421/1‑150 (MQ=255)
 aTCAACTGCAGCAACCCGAGCAGCGCCCGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTgg                                                                                                                                                 <  2:399412/150‑1 (MQ=255)
    aaCTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGccc                                                                                                                                              <  1:68227/150‑1 (MQ=255)
       tGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCggg                                                                                                                                           >  2:246815/1‑150 (MQ=255)
        gcagcaACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGa                                                                                                                                          <  2:204640/150‑1 (MQ=255)
           gcaACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACa                                                                                                                                       >  1:518553/1‑150 (MQ=255)
             aaCCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGt                                                                                                                                     >  1:359040/1‑150 (MQ=255)
               cccGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTc                                                                                                                                   <  1:245874/150‑1 (MQ=255)
                 cGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGTAACAGTTCaa                                                                                                                                 <  2:463626/150‑1 (MQ=255)
                   agcagcGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAgg                                                                                                                               >  1:200109/1‑150 (MQ=255)
                        cgcCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGGCCTGGCCCGGGAACAGTTCAAGGCCTGc                                                                                                                          <  1:717140/150‑1 (MQ=255)
                        cgcCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGc                                                                                                                          <  2:200109/150‑1 (MQ=255)
                             gCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGc                                                                                                                     <  1:460600/150‑1 (MQ=255)
                              cAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCg                                                                                                                                             >  1:501590/1‑125 (MQ=255)
                              cAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCg                                                                                                                                             <  2:501590/125‑1 (MQ=255)
                                    tGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGGCCAGGTCCgggtc                                                                                                                                         <  2:518094/123‑3 (MQ=255)
                                    tGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGGCCAGGTCCgggtc                                                                                                                                         >  1:518094/1‑121 (MQ=255)
                                        aaCTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGAcc                                                                                                          <  1:246815/150‑1 (MQ=255)
                                                         gacgaTGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGGCCCTCCAACGCCGACCCTCAGGCCTATGCCGTg                                                                                         >  2:223133/1‑150 (MQ=255)
                                                                  gcgcTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGAACCTCAGGCCTATGCCGTGCTCAagcag                                                                                <  2:11421/150‑1 (MQ=255)
                                                                      tGGTGCGGCGCCTGGGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCACCGCCGACCCTCAGGCCTAGGCCGTGCTCAAGCAGCGcc                                                                            <  2:98671/150‑1 (MQ=255)
                                                                        gTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAATGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGcc                                                                            >  2:620206/1‑148 (MQ=255)
                                                                        gTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGcc                                                                            <  1:620206/148‑1 (MQ=255)
                                                                           cggcgCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTAt                                                                       <  1:588826/150‑1 (MQ=255)
                                                                            ggcgCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATg                                                                      <  1:40898/150‑1 (MQ=255)
                                                                                 cTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGcc                                                                 <  1:394916/150‑1 (MQ=255)
                                                                                  tGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATgg                                                                     >  1:672154/1‑145 (MQ=255)
                                                                                  tGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATgg                                                                     <  2:672154/145‑1 (MQ=255)
                                                                                      cAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGGCCAGGTCCgggtct                                                                                                                                        >  2:666861/1‑71 (MQ=38)
                                                                                      cAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGGCCAGGTCCgggtct                                                                                                                                        <  1:666861/74‑4 (MQ=38)
                                                                                       aaCGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGaa                                                                                                                                         <  1:168207/72‑1 (MQ=255)
                                                                                       aaCGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGaa                                                                                                                                         >  2:168207/1‑72 (MQ=255)
                                                                                             cTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACTCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGccc                                                     <  2:311109/150‑1 (MQ=255)
                                                                                                  gcgcCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGc                                                       <  2:672387/143‑1 (MQ=255)
                                                                                                  gcgcCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGc                                                       >  1:672387/1‑143 (MQ=255)
                                                                                                           ccTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGCCCCCGCAGCCGCGCAc                                       >  1:707246/1‑150 (MQ=255)
                                                                                                               gAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGCCCCCGCAGCCGCGCAccgcc                                   >  1:622110/1‑150 (MQ=255)
                                                                                                                          cTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCATATTAATTGCCCATCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGCCCCCGCAGCCGCGCACCGCCGCAGAATGGGa                        <  2:331557/150‑1 (MQ=255)
                                                                                                                           tGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGCCCCCGCAGCCGCGCACCGCCGCAGAATGGGaa                       <  2:555963/150‑1 (MQ=255)
                                                                                                                               tGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGCCCCCGCAGCCGCGCACCGCCGCAGAATGGGaacaac                   >  1:757417/1‑150 (MQ=255)
                                                                                                                                     aTCACGCCCCGGACCTGGCCTGGGCACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGTCCCCACAGCCGCGCACCGCCGCAGAATGGGAACAACGCTATc             <  2:518553/150‑1 (MQ=255)
                                                                                                                                     aTCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAagc                                                                                  <  2:207433/81‑1 (MQ=255)
                                                                                                                                     aTCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAagc                                                                                  >  1:207433/1‑81 (MQ=255)
                                                                                                                                        tgttcccGGGCCAGGTCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGCCCCCGCAGCCGCGCACCGCCGCAGAATGGGAACAACGCTATCGGc          >  2:322846/5‑150 (MQ=255)
                                                                                                                                            cccGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAagcagc                                                                               <  1:693635/77‑1 (MQ=255)
                                                                                                                                            cccGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAagcagc                                                                               >  2:693635/1‑77 (MQ=255)
                                                                                                                                            cccGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGACCCGGCAGCCGCGCACCGCCGCAGAATGGGAACAACGCTATGGGCGGag      >  1:388833/1‑149 (MQ=255)
                                                                                                                                             ccGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTAt                                                                       >  2:381251/1‑84 (MQ=255)
                                                                                                                                             ccGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTAt                                                                       <  1:381251/84‑1 (MQ=255)
                                                                                                                                             ccGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGCCCCCGCAGCCGCGCACCGCCGCAGAATGGGAACAACGCTATCGGCAGAcc     >  1:680747/1‑150 (MQ=255)
                                                                                                                                             ccGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGCCCCCGCAGCCGCGCACCGCCGCAGAATGGGAACAACGAGATCGGAAGagc     >  1:331920/1‑148 (MQ=255)
                                                                                                                                              cGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGCCCCCGCAGCCGCGCAGCGCCGCAGAATGGGAACAACGCTATCGGCAGACCg    >  2:458648/1‑150 (MQ=255)
                                                                                                                                                gACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGCCCCCGCAGCCGCGCACCGCCGCAGAATGGGAACAACGCTATCGGCAGACCGGc  >  1:488902/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAGCAACGACCTGCAGCGCCCCTGCCTTGAAACGGTCGACTGGCTGTCACATCAAGACCCGGACCTGGCCCGGGAACAGTTCAAGGCCTGCCCCGCCAACGCCGACCCTCAGGCCTATGCCGTGCTCAAGCAGCGCCTGTATGGCGCCCCGCCGCAGCCCCCGCAGCCGCGCACCGCCGCAGAATGGGAACAACGCTATCGGCAGACCGGC  >  NC_002947/3908522‑3908815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑