Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,170,489 T→G 12.2% L56R (CTG→CGG)  PP_3669 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,170,4890TG12.2% 93.8 / 8.0 41L56R (CTG→CGG) PP_3669LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (21/15);  new base G (2/3);  total (23/18)
Fisher's exact test for biased strand distribution p-value = 6.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-01

CATGAACCTGCTGGTGCTGTTCGAAGCCCTGATGATCGAACGCAACCTGACCCGCGTGGGTGAAAAGCTGTTTATCACCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTGCTGAACTGGGCCCGGCCATGGACGTGATTTCCGCTGCCATCAGCCGCGCCCGCGAGTTCGACCCGGCCAACAGCTGCAACGTGTTCCGCCTGG  >  NC_002947/4170352‑4170635
                                                                                                                                         |                                                                                                                                                  
cATGAACCTGCTGGTGCTGTTCGAAGCCCTGATGATCGAACGCAACCTGACCCGCGTGGGTGAAAAGCTGTTTATCACCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCGGATCAGCAGCgg                                                                                                                                        <  2:501104/150‑1 (MQ=255)
   gAACCTGCTGGTGCTGTTCGAAGCCCTGATGATCGAACGCAACCTGACCCGCGTGGGTGAAAAGCTGTTTATAACCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCg                                                                                                                                     >  2:342666/1‑150 (MQ=255)
    aaCCTGCTGGTGCTGTTCGAAGCCCTGATGATCGAACGCAACCTGACCCGCGTGGGTGAAAAGCTGTTTATCACCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCgg                                                                                                                                    >  2:57971/1‑150 (MQ=255)
    aaCCTGCTGGTGCTGTTCGAAGCCCTGATGATCGAACGCAACCTGACCCGCGTGGGTGAAAAGCTGTTTATCACCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCgg                                                                                                                                    >  2:4247/1‑150 (MQ=255)
                cTGTTCGAAGCCCTGATGATCGAACGCAACCTGACCCGCGTGGGTGAAAAGCTGTTTATCACCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAaccc                                                                                                                        >  2:107746/1‑150 (MQ=255)
                   ttCGAAGCCCTGATGATCGAACGCAACCTGACCCGCGTGGGTGAAAAGCTGTTTATCACCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAacccacc                                                                                                                     <  1:575846/150‑1 (MQ=255)
                      gAAGCCCTGATGATCGAACGCAACCTGACCCGCGTGGGTGAAAAGCTGTTTATCACCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAAcccacccca                                                                                                                  >  2:348417/1‑150 (MQ=255)
                           ccTGATGATCGAACGCAACCTGACCCGCGTGGGTGAAAAGCTGTTTATCACCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGc                                                                                                             <  1:388770/150‑1 (MQ=255)
                            cTGATGATCGAACGCAACCTGACCCGCGTGGGTGAAAAGCTGTTTATCACCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGcc                                                                                                            >  2:384424/1‑150 (MQ=255)
                             tgatgaTCGAACGCAACCTGACCCGCGTGGGTGAAAAGCTGTTTATCACCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCGGATCAGCAGCgggtc                                                                                                                                     <  1:115681/124‑4 (MQ=255)
                             tgatgaTCGAACGCAACCTGACCCGCGTGGGTGAAAAGCTGTTTATCACCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCGGATCAGCAGCgggtc                                                                                                                                     >  2:115681/1‑121 (MQ=255)
                                        cGCAACCTGACCCGCGTGGGTGAAAAGCTGTTTATCACCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCGGATCAGCAGCggg                                                                                                                                       <  2:217804/111‑2 (MQ=255)
                                        cGCAACCTGACCCGCGTGGGTGAAAAGCTGTTTATCACCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCGGATCAGCAGCggg                                                                                                                                       >  1:217804/1‑110 (MQ=255)
                                         gctaCCTGACCCGCGTGGGTGAAAAGCTGTTTATCACCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTg                                                                                               >  2:145289/4‑150 (MQ=255)
                                         gCAACCTGACCCGCGTGGGTGAAAAGCTGTTTATCACCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCa                                                                                                       <  2:841871/142‑1 (MQ=255)
                                         gCAACCTGACCCGCGTGGGTGAAAAGCTGTTTATCACCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCa                                                                                                       >  1:841871/1‑142 (MQ=255)
                                               tGACCCGCGTGGGTGAAAAGCTGTTTATCACCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTGCTGAAc                                                                                         >  2:801698/1‑150 (MQ=255)
                                                       ttGGGTGATAAACAGCTTTTCACCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTGCTGAACTGGGCCCg                                                                                 >  1:626479/2‑150 (MQ=255)
                                                       gTGGGTGAACAGCTGTTTATCACCCAATCCGCCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCGCGGGCCATGCAGATCTTTGCTGAACTGGGCCCg                                                                                 <  2:849238/150‑1 (MQ=255)
                                                           gTGAAAAGCTGTTTATCACCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTGCTGAACTGGGCCCGGCCa                                                                             >  1:389812/1‑150 (MQ=255)
                                                                  gCTGTTTATCACCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGGTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTGCTGAACTGGGCCCGGCCATGGACGt                                                                      <  1:360355/150‑1 (MQ=255)
                                                                  gCTGTTTATCACCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTGCTGAACTGGGCCCGGCCATGGACGt                                                                      <  1:348417/150‑1 (MQ=255)
                                                                           cACCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTGCTGAACTGGGCCCGGCCATGGACGTGATTTCCGc                                                             <  2:626479/150‑1 (MQ=255)
                                                                            aCCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGTCTACTCGCTGCTGATCCGCAGCGGCGGCGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTGCTGAACTGGGCCCGGCCATGGACGTGATTTCCGCt                                                            <  1:57971/150‑1 (MQ=255)
                                                                            aCCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTGCTGAACTGGGCCCGGCCATGGACGTGATTTCCGCt                                                            >  2:635588/1‑150 (MQ=255)
                                                                             cccAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAacccaccc                                                                                                                    <  1:238946/93‑1 (MQ=255)
                                                                             cccAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAacccaccc                                                                                                                    >  2:238946/1‑93 (MQ=255)
                                                                                         tCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTGCTGAACTGGGCCCGGCCATGGACGTGATTTCCGCTGCCATCAGCcgcg                                               >  1:500196/1‑150 (MQ=255)
                                                                                                cgccCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACgg                                                                                                               <  1:220398/79‑1 (MQ=255)
                                                                                                cgccCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACgg                                                                                                               >  2:220398/1‑79 (MQ=255)
                                                                                                cgccCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTGCTGAACTGGGCCCGGCCATGGACGTGATTTCCGCTGCCATCAGCCGCGCCCGCGa                                        <  1:384424/150‑1 (MQ=255)
                                                                                                    cTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTGCTGAACTGGGCCCGGCCATGGACGTGATTTCCGCTGCCATCAGCCGCGCCCGCGAGTTc                                    >  1:287231/1‑150 (MQ=255)
                                                                                                       gcccgccTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTGCTGAACTGGGCCCGGCCATGGACGTGATTTCCGCTGCCATCAGCCGCGCCCGCGAGTTCGAc                                 >  2:45920/1‑150 (MQ=255)
                                                                                                           gccTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTGCTGAACTGGGCCCGGCCATGGACGTGATTTCCGCTGCCATCAGCCGCGCCCGCGAGTTCGACCCgg                             >  2:253358/1‑150 (MQ=255)
                                                                                                            ccTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTGCTGAACTGGGCCCGGCCATGGACGTGATTTCCGCTGCCATCAGCCGCGCCCGCGAGTTCGACCCGGc                            <  1:635588/150‑1 (MQ=255)
                                                                                                                        gTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTGCTGAACTGGGCCCGGCCATGGACGTGATTTCCGCTGCCATCAGCCGCGCCCGCGAGTTCGa                                  <  2:40906/132‑1 (MQ=255)
                                                                                                                        gTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTGCTGAACTGGGCCCGGCCATGGACGTGATTTCCGCTGCCATCAGCCGCGCCCGCGAGTTCGa                                  >  1:40906/1‑132 (MQ=255)
                                                                                                                        gTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTGCTGAACTGGGCCCGGCCATGGACGTGATTTCCGCTGCCATCAGCCGCGCCCGCGAGTTCGACCCGGCCAACAGCTGCaa                <  2:500196/150‑1 (MQ=255)
                                                                                                                            gacgacCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTGCTGAACTGGGCCCGGCCATGGACGTGATTTCCGCTGCCATCAGCCGCGCCCGCGAGTTCGACCCGGCCAACAGCTGCAACgtg            >  2:49048/1‑150 (MQ=255)
                                                                                                                               gacCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTGCTGAACTGGGCCCGGCCATGGACGTGATTTCCGCTGCCATCAGCCGGGCCCGCGCGTTCGACCCGGCCAACAGCTGCAACGTGTTc         >  1:410385/1‑150 (MQ=255)
                                                                                                                               gacCCCCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTGCTGAACTGGGCCCGGCCATGGACGTGATTTCCGCTGCCATCAGCCGCGCCCGCGAGTTCGACCCGGCCAACAGCTGCAACGTGTTc         <  1:342666/150‑1 (MQ=255)
                                                                                                                                    gctgctGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTGCTGAACTGGGCCCGGCCATGGACGTGATTTCCGCTGCCATCAGCCGCGCCCGCGAGTTCGACCCGGCCAACAGCTGCAACGTGTTCCGCCt    <  1:253358/150‑1 (MQ=255)
                                                                                                                                      tgctgATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTGCTGAACTGGGCCCGGCCATGGACGTGATTTCCGCTGCCATCAGCCGCGCCCGCGAGTTCGACCCGGCCAACAGCTGCAACGTGTTCCGCCTgg  <  1:145289/150‑1 (MQ=255)
                                                                                                                                      tgctgATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTGCTGAACTGGGCCCGGCCATGGACGTGATTTCCGCTGCCATCAGCCGCGCCCGCGAGTTCGACCCGGCCAACAGCTGCAACGTGTTCCGCCTgg  <  2:389812/150‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                                  
CATGAACCTGCTGGTGCTGTTCGAAGCCCTGATGATCGAACGCAACCTGACCCGCGTGGGTGAAAAGCTGTTTATCACCCAATCCACCGTCAGCGCCGCCCTCGCCCGCCTGCGCGAGCTGTTCGACGACCCGCTGCTGATCCGCAGCGGCCGGGCCATGGAACCCACCCCACGGGCCATGCAGATCTTTGCTGAACTGGGCCCGGCCATGGACGTGATTTCCGCTGCCATCAGCCGCGCCCGCGAGTTCGACCCGGCCAACAGCTGCAACGTGTTCCGCCTGG  >  NC_002947/4170352‑4170635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑