Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,231,788 C→A 10.0% intergenic (‑4/+80) PP_3709 ← / ← PP_5625 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,231,7880CA10.0% 115.0 / 3.6 40intergenic (‑4/+80)PP_3709/PP_5625hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (17/19);  new base A (2/2);  total (19/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

ACTACCAAGGTAGGCAGTAATGGGCTTGTCGAAGCCCTGATGTTTCGCTTTGGCAATCCGCCTCAGATCTAGAACGCTTAAGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATAGACACCTCTGAGACGCGGTAGAGGGTCTGCAGTCTACTCTGCACGGATCATCGCCGATAGGAAGATAGGCCTTTGGTTGGT  >  NC_002947/4231665‑4231915
                                                                                                                           |                                                                                                                               
aCTACCAAGGTAGGCAGTAATGGGCTTGTCGAAGCCCTGATGTTTCGCTTTGGCAATCCGCCTCAGATCTAGAACGCTTAAGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCt                                                                                                       >  2:126159/1‑150 (MQ=255)
  tACCAAGGTAGGCAGTAATGGGCTTGTCGAAGCCCTGATGTTTCGCTTTGGCAATCCGCCTCAGATCTAGAACGCTTAAGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTa                                                                                                                              <  2:18041/125‑1 (MQ=255)
  tACCAAGGTAGGCAGTAATGGGCTTGTCGAAGCCCTGATGTTTCGCTTTGGCAATCCGCCTCAGATCTAGAACGCTTAAGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTa                                                                                                                              >  1:18041/1‑125 (MQ=255)
    ccAAGGTAGGCAGTAATGGGCTTGTCGAAGCCCTGATGTTTCGCTTTGGCAATCCGCCTCAGATCTAGAACGCTTAAGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGc                                                                                                   >  1:285935/1‑150 (MQ=255)
     cAAGGTAGGCAGTAATGGGCTTGTCGAGGCCCTGATGTTTCGCTTTGGCAATCCGCCTCAGATCTAGAACGCTTAAGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGCt                                                                                                  >  1:240608/1‑150 (MQ=255)
              cAGTAATGGGCTTGTCGAAGCCCTGATGTTTCGCTTTGGCAATCCGCCTCAGATCTAGAACGCTTAAGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACAcc                                                                                         <  2:285935/150‑1 (MQ=255)
                   aTGGGCTTGTCGAAGCCCTGATGTTTCGCTTTGGCAATCCGCCTCAGATCTAGAACGCTTAAGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAAt                                                                                    >  1:369021/1‑150 (MQ=255)
                           gTCGAAGCCCTGATGTTTCGCTTTGGCAATCCGCCTCAGATCTAGAACGCTTAAGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATAGACACct                                                                            <  1:217867/150‑1 (MQ=255)
                                aGCCCTGATGTTTCGCTTTGGCAATCCGCCTCAGATCTAGAACGCTTAAGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATAGACACCTCTgag                                                                       <  1:186295/150‑1 (MQ=255)
                                    ccgATGTTTCGCTTTGGCAAGCCGCTTCAGATCTAGAACGCTTAAGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATAGACACCTCTGAGAcgc                                                                   <  2:332955/148‑1 (MQ=255)
                                           ttCGCTTTGGCAATCCGCCTCAGATCTAGAACGCTTAAGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAAt                                                                                                                   <  1:524783/95‑1 (MQ=255)
                                           ttCGCTTTGGCAATCCGCCTCAGATCTAGAACGATTAAGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAAt                                                                                                                   >  2:524783/1‑95 (MQ=255)
                                               cTTTGGCAATCCGCCTCAGATCTAGAACGCTTAAGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTcc                                                                                                     >  1:483628/1‑105 (MQ=255)
                                               cTTTGGCAATCCGCCTCAGATCTAGAACGCTTAAGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTcc                                                                                                     <  2:483628/105‑1 (MQ=255)
                                               cTTTGGCAATCCGCCTCAGATCTAGAACGCTTAAGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATAGACACCTCTGAGACGCGGTAGAGGGTc                                                        <  1:126159/150‑1 (MQ=255)
                                                  tGGCAATCCGCCTCAGATCTAGAACGCTTAAGCTCTTGCCGTGAAGACTCAGTTCAACCGGTTCCTGCATTTTACTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATAGACACCTCTGAGACGCGGTAGAGGGTCTGc                                                     >  2:807531/1‑150 (MQ=255)
                                                  tGGCAATCCGCCTCAGATCTAGAACGCTTAAGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTACTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATAGACACCTCTGAGACGCGGTAGAGGGTCTGc                                                     >  2:807353/1‑150 (MQ=255)
                                                   ggCAATCCGCCTCAGATCTAGAACGCTTAAGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGAACAAGACCGTCGCGTCCGCTAGAGACACCGAAATAGACACCTCTGAGACGCGGTAGAGGGTCTGCa                                                    <  2:543043/150‑1 (MQ=255)
                                                           gCCTCAGATCTAGAACGCTTAAGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATAGACACCTCTGAGACGCGGTAGAGGGTCTGCAGTCTActc                                            <  2:369021/150‑1 (MQ=255)
                                                              tCAGATCTAGAACGCTTAAGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATAGACACCTCTGAGACGCGGTAGAGGGTCTGCAGTCTACTCTGc                                         >  1:89626/1‑150 (MQ=255)
                                                              tCAGATCTAGAACGCTTAAGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATAGACACCTCTGAGACGCGGGAGAGGGTCTGCAGTCTaagatcg                                         >  1:137081/1‑144 (MQ=255)
                                                                       gAACGCTTAAGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATa                                                                                   <  2:785233/99‑1 (MQ=255)
                                                                       gAACGCTTAAGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATa                                                                                   >  1:785233/1‑99 (MQ=255)
                                                                             ttAAGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATAGACACCTCTGAGACGCGGTAGAGGGTCTGCAGTCTACTCTGCACGGATCATCGCCGa                          >  1:827574/1‑150 (MQ=255)
                                                                                aGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCATACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATAGACACCTCTGAGACGCGGTAGAGGGTCTGCAGTCTACTCTGCACGGATCATCGCCGATAg                       <  2:240608/150‑1 (MQ=255)
                                                                                 gCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGc                                                                                                         <  2:160366/67‑1 (MQ=255)
                                                                                 gCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGc                                                                                                         >  1:160366/1‑67 (MQ=255)
                                                                                  ctctTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATAGACACCTCTGAGACGCGGTAGAGGGTCTGCAGTCTACTCTGCACGGATCATCGCCGATAGGa                     <  2:827574/150‑1 (MQ=255)
                                                                                     ttGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATAGACACCTCTGAGACGCGGTAGAGGGTCTGCAGTCTACTCTGCACGGATCATCGCCGATAGGAAGa                  <  2:89626/150‑1 (MQ=255)
                                                                                        ccGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATAGACACCTCTGAGACGCGGTAGAGGGTCTGCAGTCTACTCTGCACGGATCATCGCCGATAGGa                     <  1:110119/144‑1 (MQ=255)
                                                                                        ccGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATAGACACCTCTGAGACGCGGTAGAGGGTCTGCAGTCTACTCTGCACGGATCATCGCCGATAGGa                     >  2:110119/1‑144 (MQ=255)
                                                                                                    aGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATAGac                                                                                <  2:91342/73‑1 (MQ=255)
                                                                                                    aGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATAGac                                                                                >  1:91342/1‑73 (MQ=255)
                                                                                                    aGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATAGACACctct                                                                          <  2:541943/79‑1 (MQ=255)
                                                                                                    aGTTCAACCGGTTCCTGCATTTTCATACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATAGACACctct                                                                          >  1:541943/1‑79 (MQ=255)
                                                                                                    aGTTCAACCGGTTCCTGCATTTTACTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATAGACACCTCTGAGACGCGGTAGAGGGTCTGCAGTCTACTCTGCACGGATCATCGCCGATAGGAAGATAGGCCTttggttgg   <  1:807531/150‑1 (MQ=255)
                                                                                                    aGTTCAACCGGTTCCTGCATTTTACTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATAGACACCTCTGAGACGCGGTAGAGGGTCTGCAGTCTACTCTGCACGGATCATCGCCGATAGGAAGATAGGCCTttggttgg   <  1:807353/150‑1 (MQ=255)
                                                                                                     gTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATAGACACCTCTGAGACGCGGTAGAGGGTCTGCAGTCTACTCTGAAGGGATCATCGCCGATAGGAAGATAGGCCTTtggttggt  >  1:225038/1‑150 (MQ=255)
                                                                                                              gTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGCTagag                                                                                              <  2:121873/49‑1 (MQ=255)
                                                                                                              gTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGCTagag                                                                                              >  1:121873/1‑49 (MQ=255)
                                                                                                                           |                                                                                                                               
ACTACCAAGGTAGGCAGTAATGGGCTTGTCGAAGCCCTGATGTTTCGCTTTGGCAATCCGCCTCAGATCTAGAACGCTTAAGCTCTTGCCGTGAAAACTCAGTTCAACCGGTTCCTGCATTTTCCTACTCCATGTAATCGCTAGACGCCTCCGCTAGAGACACCGAAATAGACACCTCTGAGACGCGGTAGAGGGTCTGCAGTCTACTCTGCACGGATCATCGCCGATAGGAAGATAGGCCTTTGGTTGGT  >  NC_002947/4231665‑4231915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑