Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,248,764 G→T 8.6% A436D (GCT→GAT)  PP_3724 ← acyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,248,7640GT 8.6% 181.8 / 4.0 57A436D (GCT→GAT) PP_3724acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base G (22/30);  new base T (3/2);  total (25/32)
Fisher's exact test for biased strand distribution p-value = 6.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTC  >  NC_002947/4248616‑4248903
                                                                                                                                                    |                                                                                                                                           
gTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGc                                                                                                                                            <  1:303429/150‑1 (MQ=255)
    aaCAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATg                                                                                                                                        <  1:252031/150‑1 (MQ=255)
     aCAGTCGGCTTTCGCCCGTGCGGTCCTGCAGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGc                                                                                                                                       <  2:835089/150‑1 (MQ=255)
      cAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGcc                                                                                                                                      <  2:179426/150‑1 (MQ=255)
        gTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCATGCACGGCATCGCTGccct                                                                                                                                    >  2:370892/1‑149 (MQ=255)
          cGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCcgc                                                                                                                                  <  2:443532/150‑1 (MQ=255)
                 cGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCGTCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCATCTAGGCCGTCTTCCACACCCTGCAGGGCATCGCTGCCCtgcagggtgt                                                                                                                           <  1:370892/150‑8 (MQ=255)
                  gCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCTATCAACCGCTGGCTGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAg                                                                                                                          <  1:616980/150‑1 (MQ=255)
                  gCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCATCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAg                                                                                                                          <  2:49278/150‑1 (MQ=255)
                   cccGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAgg                                                                                                                         >  2:241789/1‑150 (MQ=255)
                    ccGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGgc                                                                                                                        <  1:168121/150‑1 (MQ=255)
                    ccGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCACGGCCTCGATGCCCCGCGCATGCAGgc                                                                                                                        >  2:376034/1‑150 (MQ=255)
                         aGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCa                                                                                                                   <  1:241789/150‑1 (MQ=255)
                            gCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCAcgc                                                                                                                <  1:216806/150‑1 (MQ=255)
                              cTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACgcgc                                                                                                              >  1:185244/1‑150 (MQ=255)
                               tGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCa                                                                                                             >  1:297279/1‑150 (MQ=255)
                                    gCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTggc                                                                                                        >  2:336015/1‑150 (MQ=255)
                                       caGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTggcagg                                                                                                     <  2:695157/150‑1 (MQ=255)
                                            gTACCCGAAAAGCCTCGCCCTGGTACCTCACCCGCTGGCGGTTCTTTTGGTACGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACa                                                                                                <  2:180467/150‑1 (MQ=255)
                                                ccGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAg                                                                                                                          <  1:63227/120‑1 (MQ=255)
                                                ccGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAg                                                                                                                          >  2:63227/1‑120 (MQ=255)
                                                ccGCACAGCCTCGCCCTGGGCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAg                                                                                                                          <  1:63224/120‑1 (MQ=255)
                                                ccGCACAGCCTCGCCCTGGGCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAg                                                                                                                          >  2:63224/1‑120 (MQ=255)
                                                 cGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCATCGCTGccctgca                                                                                                                                 >  1:49053/1‑108 (MQ=255)
                                                 cGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCATCGCTGCCCtgca                                                                                                                                 <  2:49053/112‑2 (MQ=255)
                                                 cGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTcc                                                                                           >  2:330426/1‑150 (MQ=255)
                                                  gCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCg                                                                                          <  1:569339/150‑1 (MQ=255)
                                                       gCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGaaa                                                                                     <  2:486507/150‑1 (MQ=255)
                                                       gCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGAAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGaaa                                                                                     <  1:472657/150‑1 (MQ=255)
                                                          tCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGcc                                                                                  <  1:336015/150‑1 (MQ=255)
                                                                    ccATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGa                                                                        >  1:196821/1‑150 (MQ=255)
                                                                       tCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAg                                                                               <  1:778603/140‑1 (MQ=255)
                                                                       tCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAg                                                                               >  2:778603/1‑140 (MQ=255)
                                                                       tCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGc                                                                     >  2:481853/1‑150 (MQ=255)
                                                                            cGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATc                                                                <  1:330426/150‑1 (MQ=255)
                                                                                 gCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGa                                                                                <  1:314877/129‑1 (MQ=255)
                                                                                 gCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGa                                                                                >  2:314877/1‑129 (MQ=255)
                                                                                   ggTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGt                                                         >  2:264103/1‑150 (MQ=255)
                                                                                   ggTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGt                                                         <  2:396765/150‑1 (MQ=255)
                                                                                            ggTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCaa                                                >  1:273892/1‑150 (MQ=255)
                                                                                              tAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACg                                              >  2:686654/1‑150 (MQ=255)
                                                                                                            cccccAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAg                                <  2:273892/150‑1 (MQ=255)
                                                                                                             ccccAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTcc                                                                                           >  2:544190/1‑90 (MQ=255)
                                                                                                             ccccAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTcc                                                                                           <  1:544190/90‑1 (MQ=255)
                                                                                                              cccAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGcc                              >  2:154825/1‑150 (MQ=255)
                                                                                                                      gccaggccgTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCcagcagc                      <  1:154825/150‑1 (MQ=255)
                                                                                                                      gccaggccgTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCcagcaac                      >  2:47963/1‑148 (MQ=255)
                                                                                                                      gccaggccgTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGAAGTCCAGCCCcagcagc                      >  2:47918/1‑150 (MQ=255)
                                                                                                                            ccgTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCg                <  1:686654/150‑1 (MQ=255)
                                                                                                                             cgTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGc               >  2:219157/1‑150 (MQ=255)
                                                                                                                              gTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCa              <  1:481853/150‑1 (MQ=255)
                                                                                                                                    gacacCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCtt        >  2:461874/2‑150 (MQ=255)
                                                                                                                                    cacaCCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCtt        <  1:851318/150‑1 (MQ=255)
                                                                                                                                      cacCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGc      >  1:428475/1‑150 (MQ=255)
                                                                                                                                         ccTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAg                                                                               >  1:641348/1‑74 (MQ=255)
                                                                                                                                         ccTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAg                                                                               <  2:641348/74‑1 (MQ=255)
                                                                                                                                          cTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTc  <  1:264103/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                           
GTCCAACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTC  >  NC_002947/4248616‑4248903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑