Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,248,767 A→C 7.1% I435S (ATC→AGC)  PP_3724 ← acyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,248,7670AC 7.1% 158.1 / 4.6 56I435S (ATC→AGC) PP_3724acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base A (23/29);  new base C (2/2);  total (25/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01

AACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTC  >  NC_002947/4248620‑4248903
                                                                                                                                                   |                                                                                                                                        
aaCAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATg                                                                                                                                        <  1:252031/150‑1 (MQ=255)
 aCAGTCGGCTTTCGCCCGTGCGGTCCTGCAGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGc                                                                                                                                       <  2:835089/150‑1 (MQ=255)
  cAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGcc                                                                                                                                      <  2:179426/150‑1 (MQ=255)
    gTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCATGCACGGCATCGCTGccct                                                                                                                                    >  2:370892/1‑149 (MQ=255)
      cGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCcgc                                                                                                                                  <  2:443532/150‑1 (MQ=255)
             cGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCGTCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCATCTAGGCCGTCTTCCACACCCTGCAGGGCATCGCTGCCCtgcagggtgt                                                                                                                           <  1:370892/150‑8 (MQ=255)
              gCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCTATCAACCGCTGGCTGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAg                                                                                                                          <  1:616980/150‑1 (MQ=255)
              gCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCATCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAg                                                                                                                          <  2:49278/150‑1 (MQ=255)
               cccGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAgg                                                                                                                         >  2:241789/1‑150 (MQ=255)
                ccGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGgc                                                                                                                        <  1:168121/150‑1 (MQ=255)
                ccGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCACGGCCTCGATGCCCCGCGCATGCAGgc                                                                                                                        >  2:376034/1‑150 (MQ=255)
                     aGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCa                                                                                                                   <  1:241789/150‑1 (MQ=255)
                        gCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCAcgc                                                                                                                <  1:216806/150‑1 (MQ=255)
                          cTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACgcgc                                                                                                              >  1:185244/1‑150 (MQ=255)
                           tGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCa                                                                                                             >  1:297279/1‑150 (MQ=255)
                                gCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTggc                                                                                                        >  2:336015/1‑150 (MQ=255)
                                   caGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTggcagg                                                                                                     <  2:695157/150‑1 (MQ=255)
                                        gTACCCGAAAAGCCTCGCCCTGGTACCTCACCCGCTGGCGGTTCTTTTGGTACGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACa                                                                                                <  2:180467/150‑1 (MQ=255)
                                            ccGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAg                                                                                                                          <  1:63227/120‑1 (MQ=255)
                                            ccGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAg                                                                                                                          >  2:63227/1‑120 (MQ=255)
                                            ccGCACAGCCTCGCCCTGGGCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAg                                                                                                                          <  1:63224/120‑1 (MQ=255)
                                            ccGCACAGCCTCGCCCTGGGCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAg                                                                                                                          >  2:63224/1‑120 (MQ=255)
                                             cGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCATCGCTGccctgca                                                                                                                                 >  1:49053/1‑108 (MQ=255)
                                             cGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCATCGCTGCCCtgca                                                                                                                                 <  2:49053/112‑2 (MQ=255)
                                             cGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTcc                                                                                           >  2:330426/1‑150 (MQ=255)
                                              gCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCg                                                                                          <  1:569339/150‑1 (MQ=255)
                                                   gCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGaaa                                                                                     <  2:486507/150‑1 (MQ=255)
                                                   gCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGAAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGaaa                                                                                     <  1:472657/150‑1 (MQ=255)
                                                      tCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGcc                                                                                  <  1:336015/150‑1 (MQ=255)
                                                                ccATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGa                                                                        >  1:196821/1‑150 (MQ=255)
                                                                   tCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAg                                                                               <  1:778603/140‑1 (MQ=255)
                                                                   tCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAg                                                                               >  2:778603/1‑140 (MQ=255)
                                                                   tCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGc                                                                     >  2:481853/1‑150 (MQ=255)
                                                                        cGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATc                                                                <  1:330426/150‑1 (MQ=255)
                                                                             gCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGa                                                                                >  2:314877/1‑129 (MQ=255)
                                                                             gCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGa                                                                                <  1:314877/129‑1 (MQ=255)
                                                                               ggTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGt                                                         >  2:264103/1‑150 (MQ=255)
                                                                               ggTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGt                                                         <  2:396765/150‑1 (MQ=255)
                                                                                        ggTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCaa                                                >  1:273892/1‑150 (MQ=255)
                                                                                          tAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACg                                              >  2:686654/1‑150 (MQ=255)
                                                                                                        cccccAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAg                                <  2:273892/150‑1 (MQ=255)
                                                                                                         ccccAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTcc                                                                                           <  1:544190/90‑1 (MQ=255)
                                                                                                         ccccAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTcc                                                                                           >  2:544190/1‑90 (MQ=255)
                                                                                                          cccAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGcc                              >  2:154825/1‑150 (MQ=255)
                                                                                                                  gccaggccgTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCcagcagc                      <  1:154825/150‑1 (MQ=255)
                                                                                                                  gccaggccgTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCcagcaac                      >  2:47963/1‑148 (MQ=255)
                                                                                                                  gccaggccgTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGAAGTCCAGCCCcagcagc                      >  2:47918/1‑150 (MQ=255)
                                                                                                                        ccgTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCg                <  1:686654/150‑1 (MQ=255)
                                                                                                                         cgTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGc               >  2:219157/1‑150 (MQ=255)
                                                                                                                          gTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCa              <  1:481853/150‑1 (MQ=255)
                                                                                                                                gacacCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCtt        >  2:461874/2‑150 (MQ=255)
                                                                                                                                cacaCCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCtt        <  1:851318/150‑1 (MQ=255)
                                                                                                                                  cacCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGc      >  1:428475/1‑150 (MQ=255)
                                                                                                                                     ccTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAg                                                                               >  1:641348/1‑74 (MQ=255)
                                                                                                                                     ccTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAg                                                                               <  2:641348/74‑1 (MQ=255)
                                                                                                                                      cTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTc  <  1:264103/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                        
AACAGTCGGCTTTCGCCCGTGAGGGCCTGCGGGCACAGCGGTACCCGCACAGCCTCGCCCTGGTCCATCAACCGCTGGCGGTTCTTTTGGTAAGCGACGATGCGCCCCCAGGCCGCCAGGCCGTCTTCCACACCCTGCAGGGCAGCGATGCCCCGCGCATGCAGGCGCTCACGCGCACTGGCAGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTC  >  NC_002947/4248620‑4248903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑