| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,264,433 | G→T | 10.1% | V228L (GTG→TTG) | vanA → | vanillate O‑demethylase oxygenase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,264,433 | 0 | G | T | 10.1% | 137.6 / 3.8 | 47 | V228L (GTG→TTG) | vanA | vanillate O‑demethylase oxygenase subunit |
| Reads supporting (aligned to +/- strand): ref base G (22/20); new base T (3/2); total (25/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
TCACCGGCGACGAAGTGGTCACCGCCCGGCACATGGAAAACATCATGGCGCCACCGTTCTGGCGCATGGCCTTGCGTGGCAATGGCCTGGCCGACGATGTACCAGTGGACCGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGAGCATCGTGGTCGACTTCATCACCCCTGAGAGCGATACCTCTATCTGGTACTTCTGGGGCATGGCGCGCAACTTCGC > NC_002947/4264287‑4264581 | tCACCGGCGACGAAGTGGTCACCGCCCGGCACATGGAAAACATCATGGCGCCACCGTTCTGGCGCATGGCCTTGCGTGGCAATGGCCTGGCCGACGATGTACCAGTGGACCGCTGGCAGATCTGCCGTTTCATCCCACCTAGCCATGTGc < 1:387896/150‑1 (MQ=255) gTGGTCACCGCCCGGCACATGGAAAACATCATGGCGCCACCGTTCTGGCGCATGGCCTTGCGTGGCAATGGCCTGGCCGACGATGTACCAGTGGACCGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGgt < 1:799871/150‑1 (MQ=255) gTGGTCACCGCCCGGCACATGGAAAACATCATGGCGCCACCGTTCTGGCGCATGGCCTTGCGTGGCAATGGCCTGGCCGACGATGTACCAGTGGACCGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGgt < 2:478584/150‑1 (MQ=255) tGGTCACCGCCCGGCACATGGGAAACATCATGGCGCCATCGCTCTGGCGCATGTCCTTGCGGGGCAATGGCCTGGCCGACGATGTACCAGTGGACCGCTGGCGGATCTGCCGTTTCACCCCACCTAGCCATGTGCGGATCGAggtgggtg > 2:578069/1‑150 (MQ=255) cACCGCCCGGCACATGGAAAACATCATGGCGCCACCGTTCTGGCGCATGGCCTTGCGTGGCAATGGCCTGGCCGACGATGTACCAGTGGACCGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAgc < 1:707182/150‑1 (MQ=255) aCCGCCCGGCACATGGAAAACATCATGGCGCCACCGTTCTGGCGCATGGCCTTGCGTGGCAATGGCCTGGCCGACGATGTACCAGTGGACCGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAgcg > 1:600001/1‑150 (MQ=255) aTGGAAAACATCATGGCGCCACCGTTCTGGCGCATGGCCTTGCGTGGCAATGGCCTGGCCGACGATGTACCAGTGGACCGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGATCg < 2:211605/124‑1 (MQ=255) aTGGAAAACATCATGGCGCCACCGTTCTGGCGCATGGCCTTGCGTGGCAATGGCCTGGCCGACGATGTACCAGTGGACCGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGATCg > 1:211605/1‑124 (MQ=255) aTGGAAAACATCATGGCGCCACCGTTCTGGCGCATGGCCTTGCGTGGCAATGGCCTGGCCGACGATGTACCAGTGGACCGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAg > 1:115084/1‑150 (MQ=255) aTGGAAAACATCATGGCGCCACCGGTCTGGCGCATGGCCTTGCGTGGCAATGGCCTGGCCGACGATGTACCAGTGGACCGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAg > 2:716062/1‑150 (MQ=255) aaaaCATCATGGCGCCACCGTTCTGGCGCATGGCCTTGCGTGGCAATGGCCTGGCCGACGATGTACCAGTGGACCGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGggcg > 1:271992/1‑150 (MQ=255) atcatGGCGCCACCGTTCTGGCGCATGGCCTTGCGTGGCAATGGCCTGGCCGACGATGTACCAGTGGACCGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTAc < 2:600001/150‑1 (MQ=255) aCCGTTCTGGCGCATGGCCTTGCGTGGCAATGGCCTGGCCGACGATGTACCAGTGGACCGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGa < 1:472467/101‑1 (MQ=255) aCCGTTCTGGCGCATGGCCTTGCGTGGCAATGGCCTGGCCGACGATGTACCAGTGGACCGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGa > 2:472467/1‑101 (MQ=255) gTTCTGGCGCATGGCCTTGCGTGGCAATGGCCTGGCCGACGATGTACCAGTGGACCGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGcaca > 2:519974/1‑150 (MQ=255) gTTCTGGCGCATGGCCTTGCGTGGCAATGGCCTGGCCGACGATGTACCAGTGGACCGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGcaca > 1:21101/1‑150 (MQ=255) cATGGCCTTGCGTGGCAATGGCCTGGCCGACGATGTACCAGTGGACCGGTGGGAGATCTGCCGTTTCACCCCACCTCTCCATTTGCTGATAAAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGc > 2:331192/1‑150 (MQ=255) tGGCCTTGCGTGGCAATGGCCTGGCCGACGATGTACCAGTGGACCGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGt > 2:379150/1‑150 (MQ=255) ccTTGCGTGGCAATGGCCTGGCCGACGATGTACCAGTGGACCGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGa < 1:379150/150‑1 (MQ=255) ccTTGCGTGGCAATGGCCTGGCCGACGATGTACCAGTGGACCGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGa < 1:553804/150‑1 (MQ=255) cTTGCGTGGCAATGGCCTGGCCGACGATGTACCAGTGGACCGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGAg > 2:811467/1‑150 (MQ=255) cGTGGCAATGGCCTGGCCGACGATGTACCAGTGGACCGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGAGCATc < 1:811467/150‑1 (MQ=255) aTGGCCTGGCCGACGATGTACCAGTGGACCGCTGGCAGATGTGCCGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGCCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGAGCATCGTGGTCg > 1:812681/1‑150 (MQ=255) ctggccGACGATGTACCAGTGGACCGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGAGCATCGTGGTCGACTtc > 2:680336/1‑150 (MQ=255) tGTACCAGTGGACCGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGAACAGCCTACGGAGTCGAGCATCGTGGTCGACTTCATCACCCCTga < 1:578069/150‑1 (MQ=255) aGTGGACCGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGCGCATCGTCGTCCAATTAATGAGCGCTGAGAGCGa > 2:184345/1‑150 (MQ=255) cGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGAGc < 1:153565/111‑1 (MQ=255) cGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGAGc > 2:153565/1‑111 (MQ=255) tGGCAGATCTGCCGTTTCACCCCACCTAGCCATTTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGAGCATCGTGGTCGACTTCATCACCCCTGAGAGCGATACCTCTATc > 1:81447/1‑150 (MQ=255) cAGATCTGCCGTTTCACCCCACCTAGCCATTTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGggcggc > 2:164489/1‑72 (MQ=255) cAGATCTGCCGTTTCACCCCACCTAGCCATTTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGggcggc < 1:164489/72‑1 (MQ=255) gCCGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCTGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGAGCATCGTGGTCGACTTCATCACCCCTGAGAGCGATAc < 1:362411/133‑1 (MQ=255) gCCGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGAGCATCGTGGTCGACTTCATCACCCCTGAGAGCGATAc > 2:362411/1‑133 (MQ=255) ccGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGAGCATCGTGGTCGACTTCATCACCCCTGAGAGCGATACCTCTATCTGGTACTTCTg < 1:519974/150‑1 (MQ=255) ccGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCAAGGCACAGCATAAGGCGTCGAGCATCGTGGTCGACTTCATCACCCCTGAGAGCGATACCTCTATCTGGTACTTCTg > 1:518578/1‑150 (MQ=255) cACCCCACCTAGCCATTTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGAGCATCGTGGTCGACTTCATCACCCCTGAGAGCGATACCTCTATCTGGTACTTCTGGGGCAt < 2:81447/150‑1 (MQ=255) aCCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGAGCATCGTGGTCGACTtcat < 1:362136/107‑1 (MQ=255) aCCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGAGCATCGTGGTCGACTtcat > 2:362136/1‑107 (MQ=255) ccccACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGAGGCTACCACGCCGAGGCACAGCATAAGGCGTCGAGCATCGTGGTCGACTTCATCACCCCTGAGAGCGATACCTCTATCTGGTACTTCTGGGGCATgg < 1:716062/150‑1 (MQ=255) cccACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGAGCATCGTGGTCGACTTCATCACCCCTGAGAGCGATACct < 1:764431/125‑1 (MQ=255) cccACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGAGCATCGTGGTCGACTTCATCACCCCTGAGAGCGATACct > 2:764431/1‑125 (MQ=255) cTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGAGCATCGTGGTCGa < 2:389109/94‑1 (MQ=255) cTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGAGCATCGTGGTCGa > 1:389109/1‑94 (MQ=255) cTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGAGCATCGTGGTCGACTTCATCACCCCTGAGAGCGATACCTCTATCTGGTACTTCTGGGGCATGGCGCGCa > 2:408004/1‑150 (MQ=255) tAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGAGCATCGTGGTCGACTTCATCACCCCTGAGAGCGATACCTCTATCTGGTACTTCTGGGGCATGGCGCGCaa < 2:725307/150‑1 (MQ=255) aTGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGAGCATCGTGGTCGACTTCATCACCCCTGAGAGCGATACCTCTATCTGGTACTTCTgg > 2:264783/1‑131 (MQ=255) aTGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGAGCATCGTGGTCGACTTCATCACCCCTGAGAGCGATACCTCTATCTGGTACTTCTgg < 1:264783/131‑1 (MQ=255) tgtgCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGAGCATCGTGGTCGACTTCATCACCCCTGAGAGCGATACCTCTATCTGGTACTTCTGGGGCATGGCGCGCAACTTCGc < 2:812681/150‑1 (MQ=255) | TCACCGGCGACGAAGTGGTCACCGCCCGGCACATGGAAAACATCATGGCGCCACCGTTCTGGCGCATGGCCTTGCGTGGCAATGGCCTGGCCGACGATGTACCAGTGGACCGCTGGCAGATCTGCCGTTTCACCCCACCTAGCCATGTGCTGATCGAAGTGGGTGTAGCGCATGCCGGCAAGGGCGGCTACCACGCCGAGGCACAGCATAAGGCGTCGAGCATCGTGGTCGACTTCATCACCCCTGAGAGCGATACCTCTATCTGGTACTTCTGGGGCATGGCGCGCAACTTCGC > NC_002947/4264287‑4264581 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |