| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,273,744 | G→A | 10.8% | R59R (CGC→CGT) | glcC ← | transcriptional regulator GlcC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,273,744 | 0 | G | A | 10.8% | 91.1 / 3.8 | 37 | R59R (CGC→CGT) | glcC | transcriptional regulator GlcC |
| Reads supporting (aligned to +/- strand): ref base G (18/15); new base A (2/2); total (20/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.53e-01 | |||||||||||
CCAGCAGGTCGAACAGAGTGCGCGGTTGCGAACTGAACAGGTGCATCAAAGGCGTAGCACCCGCCTGCCCGGTGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCAAGGCCGAACGTGAA‑CAGCCAAGCTTTTCCACCAGCCGCCGCTCCGACGGCAGTGCCTGGCCCACCTTGAGCACGCCTTCGACGATAAGCCGCTCAACCCGTTCGGCCACCTGGTCGGCGACCTTGGCCTTGCCTTCAGTAC > NC_002947/4273596‑4273889 | ccAGCAGGTCAAACAGAGTGCGCGGTTGCGAACTGAACAGGTGCATCAAAGGCGTAGCACCCGCCTGCCCGGTGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTcgc < 2:103892/150‑1 (MQ=255) tCGAACAGAGTGCGCGGTTGCGAACTGAACAGGTGCATCAAAGGCGTAGCACCCGCCTGCCCGGTGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCAAGGcc > 1:427039/1‑150 (MQ=255) gAACAGAGTGCGCGGTTGCGAACTGAACAGGTGCATCAAAGGCGTAGCACCCGCCTGCCCGGTGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCAAGGCCGa > 2:297310/1‑150 (MQ=255) gAACAGAGTGCGCGGTTGCGAACTGAACAGGTGCATCAAAGGCGTAGCACCCGCCTGCCCGGTGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCAAGGCCGa > 1:582430/1‑150 (MQ=255) aaCAGAGTGCGCGGTTGCGAACTGAACAGGTGCATCAAAGGCGTAGCACCCGCCTGCCCGGTGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCAAGGCCGaa < 1:297310/150‑1 (MQ=255) gTTGCGAACTGAACAGGTGCATCAAAGGCGTAGCACCCGCCTGCCCGGTGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCAAGGCCGAACGTGAA‑CAGCCaa < 1:470086/150‑1 (MQ=255) cGAACTGAACAGGTGCATCAAAGGCGTAGCACCCGCCTGCCCGGTGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCAAGGCCGAACGTGAA‑CAGCCAAGCtt > 2:636716/1‑150 (MQ=255) gAACTGAACAGGTGCATCAAAGGCGTAGCACCCGCCTGCCCGGTGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCAAGGCCGAACGTGAA‑CAGCCAAGCttt < 1:677736/150‑1 (MQ=255) ggTGCATCAAAGGCGTAGCACCCGCCTGCCCGGTGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCAAGGCCGAACGTGAA‑CAGCCAAGCTTTTCCACCAgcc < 2:466868/150‑1 (MQ=255) gTGCATCAAAGGCGTAGCACCCGCCTGCCCGGTGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCaa < 1:197431/114‑1 (MQ=255) gTGCATCAAAGGCGTAGCACCCGCCTGCCCGGTGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCaa > 2:197431/1‑114 (MQ=255) cATCAAAGGCGTAGCACCCGCCTGCCCGGTGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCAAGGcc < 2:491968/115‑1 (MQ=255) cATCAAAGGCGTAGCACCCGCCTGCCCGGTGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCAAGGcc > 1:491968/1‑115 (MQ=255) aaaGGCGTAGCACCCGCCTACCCGATGAGAGCGGCGACGAACGGACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCAAGGCCGAACGTGAA‑CAGCCAAGCTTTTCCACCAGCCGCCGCTcc > 2:692485/1‑150 (MQ=255) ggCGTAGCACCCGCCTGCCCGGTGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCAAGGCCTAACGTGAA‑CAGCCAAGCTTTTCCACCAGCCGCCGCTCCGAc > 2:175113/1‑150 (MQ=255) gTAGCACCCGCCTGCCCGGTGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCGGGCCTGGCAGGCCCTCGCGCAGGGCCGAACGTGAA‑CAGCCAAGCTTTTCCACCAGCCGCCGCTCCGACGGc > 1:270872/1‑150 (MQ=255) aGCACCCGCCTGCCCGGTGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCAAGGCCGAACGTGAA‑CAGCCAAGCTTTTCCACCAGCCGCCGCTCCGACGGCAg > 2:681307/1‑150 (MQ=255) aCCCGCCTGCCCGGTGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCACGCAAGGCCGAACGTGAA‑CAGCCAAGCTTTTccacca < 1:777663/128‑1 (MQ=255) aCCCGCCTGCCCGGTGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCACGCAAGGCCGAACGTGAA‑CAGCCAAGCTTTTccacca > 2:777644/1‑128 (MQ=255) aCCCGCCTGCCCGGTGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCACGCAAGGCCGAACGTGAA‑CAGCCAAGCTTTTccacca > 2:777663/1‑128 (MQ=255) aCCCGCCTGCCCGGTGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCACGCAAGGCCGAACGTGAA‑CAGCCAAGCTTTTccacca < 1:777644/128‑1 (MQ=255) cccGCCTGCCCGGGGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCAAGGCCGAACGTGAA‑CAGCCAAGCTTTTCCACCAGCCGCCGCTCCGACGGCAGTGcc > 2:570146/1‑150 (MQ=255) cGCCTGCCCGGTGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCAACGGCGAACGTGAA‑CAGCCAAGCTTTTCCACCAGCCGCCGCTCCGACGGCAGTGCCTg < 2:76575/150‑1 (MQ=255) ccTGCCCGGTGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCAAGGCCGAACGTGAA‑CAGCCAAGCTTTTCCACCAGCCGCCGCTCCGACGGCAGTGCCTGGc < 1:144623/150‑1 (MQ=255) ggTGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCAAGGCCGAACGTGAA‑CAGCCAAGCTTTTCCACCAGCCGCCGCTCCGACGGCAGTGCCTGGCCCACCtt > 1:144484/1‑150 (MQ=255) tGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGTTGATGCCGCGCCCACGCAGAACGCGCAGGCCATCGCGCAAGGCCGAACGTGAA‑CAGCCAAGCTTTTCCACCAGCCGCCGCTCCGACGGCAGTGCCTGGCCCACCTTGa < 1:484523/150‑1 (MQ=255) cgaacgaaCCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCAAGGCCGAACGTGAA‑CAGCCAAGCTTTTCCACCAGCCGCCGCTCCGACGGCAGTGCCTGGCCCACCTTGAGCACGCCTTcga < 2:235451/150‑1 (MQ=255) gaacgaacCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCAAGGCCGAACGTGAA‑CAGCCAAGCTTTTCCACCAGCCGCCGCTCCGACGGCAGTGCCTGGCCCACCTTGAGCACGCCTTcgac < 2:582430/150‑1 (MQ=255) gtCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCAAGGCCGAACGTGAA‑CAGCCAAGCTTTTCCACCAGCCGCCGCTCCGACGGCAGTGCCTGGCCCACCTTGAGCACGCCTTCGACGATAAGCCGCTCAACCCGTTCGGc > 1:424206/1‑150 (MQ=255) gatgatGCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCAAGGCCGAACGTGAA‑CAGCCAAGCTTTTCCACCAGCCGCCGCTCCGACGGCAGTGCCTGGCCCACCTTGAGCACGCCTTCGACGATAAGCCGCTCAACCCGTTCGGCCAc > 2:283979/1‑150 (MQ=255) atgatgCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCAAGGCC‑AACACCAAGCAGCCAAGCTTTTCCACCAGCCGCCGCTCCGACGGCAGTGCCTGGCCCACCTTGAGCACGCCTTCGACGATAAGCCGCTCAACCCGTTCGGCCAcc < 1:692485/150‑1 (MQ=255) ccACGCAGAACGCGCAGGCCCTCGCGCAAGGCCGAACGTGAA‑CAGCCAAGCTTTTCCACCAGCCGCCGCTCCGACGGCAGTGCCTGGCCCACCTTGAGCACGCCTTCGACGATAAGCCGCTCAACCAGGTCGGCCACCTGGTCCGCGAcc > 2:198884/1‑150 (MQ=255) gAAAGCGCAGGCCCTCGCGCAAGGCCCAACGATAA‑CAGCCAAGCTTTTCCACCAGCCGCCGCTCCGACGGCAGTGCCTGGCCCACCTTGAGCACGCCTTCGACGATAAGCCGCTCAACCCGTTCGGCCACCTGGTCGGCGACCTTGgcct > 1:22505/1‑150 (MQ=255) aaCGCGCAGGCCCTCGCGCAAGGCCTAACGTGAA‑CAGCCAAGCTTTTCCACCAGCCGCCGCTCCGACGGCAGTGCCTGGCCCACCTTGAGCACGCCTTCGACGATAAGCCGCTCAACCCGTTCGGCCACCTGGTCGGCGACCTTGgcctt < 1:175113/150‑1 (MQ=255) cgcgCAGGCCCTCGCGCAAGGCCGAACGTGAA‑CAGCCAAGCTTTTCCACCAGCCGCCGCTCCGACGGCAGTGCCTGGCCCACCTTGAGCACGCCTTCGACGATAAGCCGCTCAACCCGTTCGGCCACCTGGTCGGCGACCTTGgccttgc < 2:657297/150‑1 (MQ=255) gCCCTCGCGCAAGGCCGAACGTGAA‑CATCCAAGCGTTTCCACCAGCCGCCGCTCCGACGGCAGTGCCTGGCCCACCTTGAGCACGCCTTCGACGATAAGCCGCTCAACCCGTTCGGCCACCTGGTCGGCGACCTTGGCCTTGCCTTCAGt > 1:448087/1‑150 (MQ=255) ccTCGCGCAAGGCCGAACGTGAA‑CAGCCAAGCTTTTCCACCAGCCGCCGCTCCGACGGCAGTGCCTGGCCCACCTTGAGCACGCCTTCGACGATAAGCCGCTCAACCCGTTCGGCCACCTGGTCGGCGACCTTGGCCTTGCCTTCAGTAc > 1:70034/1‑150 (MQ=255) ccTCGCGCAAGGCCGAACGTGAA‑CAGCCAAGCTTTTCCACCAGCCGCCGCTCCGACGGCAGTGCCTGGCCCACCTTGAGCACGCCTTCGACGATAAGCCGCTCAACCCGTTCGGCCACCTGGTCGGCGACCTTGGCCTTGCCTTCAGTAc < 1:6168/150‑1 (MQ=255) | CCAGCAGGTCGAACAGAGTGCGCGGTTGCGAACTGAACAGGTGCATCAAAGGCGTAGCACCCGCCTGCCCGGTGAGATCGGCGACGAACGAACCCCGCCCCTGTTCGGTGTCGATGATGCCGCGCCCACGCAGAACGCGCAGGCCCTCGCGCAAGGCCGAACGTGAA‑CAGCCAAGCTTTTCCACCAGCCGCCGCTCCGACGGCAGTGCCTGGCCCACCTTGAGCACGCCTTCGACGATAAGCCGCTCAACCCGTTCGGCCACCTGGTCGGCGACCTTGGCCTTGCCTTCAGTAC > NC_002947/4273596‑4273889 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |