Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,316,066	A→C	13.3%	A35A (GCA→GCC)	PP_3788 →	non‑ribosomal peptide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,316,066	0	A	C	13.3%	66.9 / 4.5	30	A35A (GCA→GCC)	PP_3788	non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base A (17/9);  new base C (2/2);  total (19/11)
Fisher's exact test for biased strand distribution p-value = 6.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

ATCAGGGAATCAGTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTGCGCTATCCCGTCGTT  >  NC_002947/4315919‑4316213                                                                                                                                                    |                                                                                                                                                    atcaGAGAATCACTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGc                                                                                                                                                   <  1:177730/150‑1 (MQ=255)  tcaGGGAATCAGTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCg                                                                                                                                                  <  1:792653/150‑1 (MQ=255)           cAGTCTCGTACGCCGCAGAGTGAGGACCGCAACATGAGCCAGGCAGTAGTGAGGATCAGGGGAGGGCCTGCCTACCCCGTCCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCCGCTGCATTcgat                                                                                                                                         <  2:164470/150‑2 (MQ=255)                                       gcagcaTGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAAc                                                                                                             >  1:338103/1‑150 (MQ=255)                                         agcaTGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTa                                                                                                           >  1:352297/1‑150 (MQ=255)                                                aCCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCg                                                                                                    >  1:456729/1‑150 (MQ=255)                                                                                       ccGTGCACTTCTCGCTGGTCGAATACAACGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCg                                                             >  1:196471/1‑150 (MQ=255)                                                                                          tGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGaa                                                                                                              <  1:163945/98‑1 (MQ=255)                                                                                          tGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGaa                                                                                                              >  2:163945/1‑98 (MQ=255)                                                                                             acgtctcGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCt                                                                                             >  1:850678/4‑112 (MQ=255)                                                                                             aCTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCt                                                                                             <  2:850678/112‑1 (MQ=255)                                                                                                                 aTCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTg                                   >  1:69071/1‑150 (MQ=255)                                                                                                                 aTCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTg                                   >  2:493204/1‑150 (MQ=255)                                                                                                                  tCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGa                                  >  2:589574/1‑150 (MQ=255)                                                                                                                    gAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCgggg                                                 >  1:236834/1‑133 (MQ=255)                                                                                                                    gAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCgggg                                                 <  2:236834/133‑1 (MQ=255)                                                                                                                     aGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATg                               >  1:58429/1‑150 (MQ=255)                                                                                                                                cACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGTATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCaa                    >  2:228210/1‑150 (MQ=255)                                                                                                                                cACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCaa                    <  1:493204/150‑1 (MQ=255)                                                                                                                                 aCCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAAc                   >  1:182972/1‑150 (MQ=255)                                                                                                                                 aCCGCAACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAAc                   >  2:802868/1‑150 (MQ=255)                                                                                                                                   cGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTg                 >  2:118929/1‑150 (MQ=255)                                                                                                                                     cTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTgcg               >  1:385286/1‑150 (MQ=255)                                                                                                                                         ccATCGAATGGAGCGGCATTACACCAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTGCGCTAt           <  2:338103/150‑1 (MQ=255)                                                                                                                                         ccATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACa                          >  2:26686/1‑135 (MQ=255)                                                                                                                                         ccATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACa                          <  1:26686/135‑1 (MQ=255)                                                                                                                                           gtCGAATGCCGCTGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCacac                                                                                        <  2:419388/70‑1 (MQ=39)                                                                                                                                           gtCGAATGCCGCTGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCacac                                                                                        >  1:419388/2‑71 (MQ=255)                                                                                                                                            tCGAATGCCGCTGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCACGGCTTGATGGCACCGGCGAGCGCGGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTGCGCTATccc        >  1:101517/1‑150 (MQ=255)                                                                                                                                                  gCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTGCGCTATCCCGTCGtt  <  1:802868/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    ATCAGGGAATCAGTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTGCGCTATCCCGTCGTT  >  NC_002947/4315919‑4316213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑