Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,332,737 C→T 9.7% A166V (GCA→GTA)  PP_3804 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,332,7370CT 9.7% 109.0 / 3.2 41A166V (GCA→GTA) PP_3804hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (17/20);  new base T (2/2);  total (19/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

TGCCGGGCATTGCGGTGAACGGTGACGATGCCTATGGTGCTTACCCTTGGCTCAACCCGACCAACCTCGGACGCATGGCCGACGTGGTGGCCAATGACCTGGAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTGGCCAAAGTCGACAACCTGACGGTGGTGAGCCTGTCGGAGAGGCTGGGATATCTGGTCAACGGGTTGAACCTGGATGCGGTCGAGCAGC  >  NC_002947/4332590‑4332877
                                                                                                                                                    |                                                                                                                                            
tGCCGGGCATTGCGGTGAACGGTGACGATGCCTATGGTGCTTACCCTTGGCTCAACCCGACCAACCTCGGACGCATGGCCGACGTGGTGGCCAATGACCTGGAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGTAg                                                                                                                                            >  1:131441/1‑150 (MQ=255)
tGCCGGGCATTGCGGTGAACGGTGACGATGCCTATGGTGCTTACCCTTGGCTCAACCCGACCAACCTCGGACGCATGGCCGACGTGGTGGCCAATGACCTGGAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGCAg                                                                                                                                            >  1:56366/1‑150 (MQ=255)
tGCCGGGCATTGCGGTGAACGGTGACGATGCCTATGGTGCTTAACCTCGACGCTACCCGACCAACCTCGGACGCATGGCCGACGTGGTGGCACCTGACCTGAAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGCAg                                                                                                                                            >  1:581912/1‑150 (MQ=255)
                gAACGGTGACGATGCCTATGGTGCTTACCCTTGGCTCAAACCGACCAACCTCGGACGTATGGCCGACGTGGTGGACCCTCCGCTGAAGAGCAACAGAGTCTG‑CTAGAGGGGAAAGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAAtt                                                                                                                            <  1:15978/150‑1 (MQ=255)
                   cGGTGACGATGCCTATGGTGCTTACCCTTGGCCCAACCCGACCAACCTCGGACGCATGGCCGACGTGGTGGCCAATGACCTGGAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCt                                                                                                                         <  1:603323/150‑1 (MQ=255)
                    ggTGACGATGCCTATGGTGCTTACCCTTGGCTCAACCCGACCAACCTCGGACGCATGGCCGACGTGGTGGCCAATGACCTGGAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTg                                                                                                                        >  1:214121/1‑150 (MQ=255)
                              ccTATGGTGCTTACCCTTGGCTCAACCCGACCAACCTCGGACGCATGGCCGACGTGGTGGCCAATGACCTGGAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCgg                                                                                                              >  2:815612/1‑150 (MQ=255)
                              ccTATGGTGCTTACCCTTGGCTCAACCCGACCAAACTCGGACGCATGGCCGACGTGGTGGCCAATGACCTGGAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCgg                                                                                                              <  1:137670/150‑1 (MQ=255)
                                            ccTTGGCTCAACCCGACCAACCTCGGACGCATGGCCGACGTGGTGGCCAATGACCTGGAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGAcg                                                                                                <  1:686428/150‑1 (MQ=255)
                                                  cTCAACCCGACCAACCTCGGACGCATGGCCGACGTGGTGGCCAATGACCTGGAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTg                                                                                          >  2:559304/1‑150 (MQ=255)
                                                  cTCAACCCGACCAACCTCGGACGCATGGCCGACGTGGTGGCCAATGACCTGGAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTg                                                                                          <  1:559304/150‑1 (MQ=255)
                                                   tCAACCCGACCAACCTCGGACGCATGGCCGACGTGGTGGCCAATGACCTGGAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGTAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTgg                                                                                         >  1:642564/1‑150 (MQ=255)
                                                   tCAACCCGACCAACCTCGGACGCATGGCCGACGTGGTGGCCAATGACCTGGAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTgg                                                                                         <  2:568461/150‑1 (MQ=255)
                                                      aCCCGACCAACCTCGGACGCATGGCCGACGTGGTGGCCAATGACCTGGAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGCAggg                                                                                                                                          >  2:164130/1‑98 (MQ=255)
                                                      aCCCGACCAACCTCGGACGCATGGCCGACGTGGTGGCCAATGACCTGGAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGCAggg                                                                                                                                          <  1:164130/98‑1 (MQ=255)
                                                       cccGCCCAACCTCGCACGCATGGCCGACGTGGTGGCCAATCACCTGGAGCGC‑CTGGCGCCGGACGACAAGGCAAAGAGCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGCCGCGTCTGGctgt                                                                                     <  1:183144/150‑4 (MQ=255)
                                                       cccGACCAACCTCGGACGCATGGCCGACGTGGTGGCCAATGACCTGGAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGCCGCGTCTGGctgt                                                                                     <  1:822256/150‑4 (MQ=255)
                                                         cGACCAACCTCGGACGCATGGCCGACGTGGTGGCCAATGACCTGGAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGTAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTGGctgttg                                                                                   <  2:131441/150‑6 (MQ=255)
                                                              aaCCTCGGACGCATGGCCGACGTGGTGGCCAATGACCTGGAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTGGCCAAAGTCGAc                                                                              >  2:684594/1‑150 (MQ=255)
                                                                ccTCGGACGCATGGCCGACGTGGTGGCCAATGACCTGGAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTGGCCAAAGTCGACaa                                                                            >  2:804821/1‑150 (MQ=255)
                                                                          aTGGCCGACGTGGTGGCCAATGACCTGGAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCATCAGCCAGACGCGGCTGGCCAAAGTCGACAACCTGACggtg                                                                  <  1:815612/150‑1 (MQ=255)
                                                                              ccGACGTGGTGGCCAATGACCTGGAGCGC‑CTGGCGACCGACGACAAGGCAAAGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTGGCCAAAGTCGACAACCTGACGGTGGTGa                                                              <  1:684594/150‑1 (MQ=255)
                                                                                      gtggCCAATGACCTGGAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTGGCCAAAGTCGACAACCTGACGGTGGTGAGCCTGTCg                                                      <  2:56366/150‑1 (MQ=255)
                                                                                      gtggCCAATGACCTGGAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTGGCCAAAGTCGACAACCTGACGGTGGTGAGCCTGTCg                                                      >  1:374146/1‑150 (MQ=255)
                                                                                                    ggAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTGGCCAAAGTCGACAACCTGACGGTGGTGAGCCTGTCGGAGAGGCTgg                                            >  1:556560/1‑146 (MQ=255)
                                                                                                    ggAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTGGCCAAAGTCGACAACCTGACGGTGGTGAGCCTGTCGGAGAGGCTgg                                            <  2:556560/146‑1 (MQ=255)
                                                                                                     gAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTGGCCAAAGTCGACAACCTGACGGTGGTGAGCCTGTCGGAGAGGCTGGGatat                                       >  1:439333/1‑150 (MQ=255)
                                                                                                     gAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTGGCCAAAGTCGACAACCTGACGGTGGTGAGCCTGTCGGAGAGGCTGGGatat                                       >  2:578481/1‑150 (MQ=255)
                                                                                                               gcgcCGGACGACAAGGCAAAGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTGGCCAAAGTCGACAACCTGACGGTGGTGAGCCTGTCGGAGAGGCTGGGATATCTGGTCAAc                              <  2:439333/150‑1 (MQ=255)
                                                                                                                             ggCAAAGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCgg                                                                                             <  2:254950/73‑1 (MQ=255)
                                                                                                                             ggCAAAGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCgg                                                                                             >  1:254950/1‑73 (MQ=255)
                                                                                                                                aaaGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTGGCCAAAGTCGACAACCTGACGGTGGTGAGCCTGTCGGAGAGGCTGGGATATCTGGTCAACGGGTTGAACCTGGATGc             >  2:747748/1‑150 (MQ=255)
                                                                                                                                    ttCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTGGCCAAAGTCGACAACCTGACGGTGGTGAGCCTGTCGGAGAGGCTGGGATATCTGGTCAACGGGTTGAACCTGGATGCGGTc         <  2:732021/149‑1 (MQ=255)
                                                                                                                                     tCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTGGCCAAAGTCGACAACCTGACGGTGGTGAGCCTGTCGGAGAgg                                                <  2:454524/110‑1 (MQ=255)
                                                                                                                                     tCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTGGCCAAAGTCGACAACCTGACGGTGGTGAGCCTGTCGGAGAgg                                                >  1:454524/1‑110 (MQ=255)
                                                                                                                                       cAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTGGCCAAAGTCGACAACCTGACGGTGGTGa                                                              >  1:828540/1‑94 (MQ=255)
                                                                                                                                       cAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTGGCCAAAGTCGACAACCTGACGGTGGTGa                                                              <  2:828540/94‑1 (MQ=255)
                                                                                                                                        aGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTGGCCAAAGTCGACAACCTGACGGTGGTGAGCCTGTCGGAGAGGCTGGGATATCTGGTCAACGGGTTGAACCTGGATGCGGTCGagc     >  2:264836/1‑150 (MQ=255)
                                                                                                                                           gCAACCTGGTAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTGGCCAAAGTCGACAACCTGACGGTGGTGAGCCTGTCGGAGAGGCTGGGATATCTGGTCAACGGGTTGAACCTGGATGCGGTCGagcagc  <  2:642564/150‑1 (MQ=255)
                                                                                                                                           gCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTGGCCAAAGTCGACAACCTGACGGTGGTGAGCCTGTCGGAGAGGCTGGGATATCTGGTCAACGGGTTGAACCTGGATGCGGTCGagcagc  <  2:260793/150‑1 (MQ=255)
                                                                                                                                           gCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTGGCCAAAGTCGACAACCTGACGGTGGTGAGCCTGTCGGAGAGGCTGGGATATCTGGTCAACGGGTTGAACCTGGATGCGGTCGagcagc  <  1:264836/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
TGCCGGGCATTGCGGTGAACGGTGACGATGCCTATGGTGCTTACCCTTGGCTCAACCCGACCAACCTCGGACGCATGGCCGACGTGGTGGCCAATGACCTGGAGCGC‑CTGGCGCCGGACGACAAGGCAAAGATCCAGGGCAACCTGGCAGGGCTCAAACGCCAATTGCTGGAGCTTTCGGCCAACAGCCAGACGCGGCTGGCCAAAGTCGACAACCTGACGGTGGTGAGCCTGTCGGAGAGGCTGGGATATCTGGTCAACGGGTTGAACCTGGATGCGGTCGAGCAGC  >  NC_002947/4332590‑4332877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑