| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,353,340 | C→G | 15.6% | P141R (CCG→CGG) | yrpB → | 2‑nitropropane dioxygenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,353,340 | 0 | C | G | 15.6% | 79.2 / 8.3 | 32 | P141R (CCG→CGG) | yrpB | 2‑nitropropane dioxygenase |
| Reads supporting (aligned to +/- strand): ref base C (13/14); new base G (2/3); total (15/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
AGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCTGCGATGCGATCATCGCCATGGGGTATGAAGCCGGTGGCCATCGCGGCA > NC_002947/4353205‑4353486 | aGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAActgc < 1:468174/150‑1 (MQ=255) cccTTATTACAGCGAAGTGGGCGCCAATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCAGTTCGGCCTGCGGCAGGCCGAAatgga < 2:485277/149‑3 (MQ=255) ccTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAActgctg > 1:645935/1‑150 (MQ=255) aCGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTCAAGGCCAGCGGTGCCaa > 2:440628/1‑146 (MQ=255) aCGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCaa < 1:440628/146‑1 (MQ=255) cACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAg > 1:560139/1‑150 (MQ=255) cAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAgc > 1:21684/1‑139 (MQ=255) cAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAgc < 2:21684/139‑1 (MQ=255) cAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGt < 1:843524/150‑1 (MQ=255) aaCCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTg > 1:298074/1‑150 (MQ=255) ccTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCg < 2:119787/150‑1 (MQ=255) cTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCAGTTCGGCCTGCGGCAGGCCGaaat > 1:794192/1‑83 (MQ=38) cTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCAGTTCGGCCTGCGGCAGGCCGaaat < 2:794192/85‑3 (MQ=38) cTTTCGAAGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCgg > 2:834252/1‑150 (MQ=255) gacgaGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTaaa > 1:807786/1‑95 (MQ=255) gacgaGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTaaa < 2:807786/95‑1 (MQ=255) gacgaGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACCACCGTGGAAGAAGCGGCctgg > 2:27440/1‑150 (MQ=255) gacgaGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCCGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCctgg < 2:226608/150‑1 (MQ=255) gagCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGACCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAgcg < 2:343106/150‑1 (MQ=255) gctgGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCtg > 2:787509/1‑150 (MQ=255) ttGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCTGCGATGCGatcatc < 1:27440/150‑1 (MQ=255) gcCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCTGCGATGCGATCATCGCCa > 1:122175/1‑150 (MQ=255) cccGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCTGCGATGCGATCATCGCCAt < 1:834252/150‑1 (MQ=255) cGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCTGCGATGCGATCATCGCCATgg < 1:289654/150‑1 (MQ=255) cGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCTGCGATGCGATCATCGCCATgg < 2:645935/150‑1 (MQ=255) ttCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCTGCGATGCGATCATCGCCATGGGGTATGAAGCCGGt > 1:42616/1‑150 (MQ=255) gcagttCGGCCTGCGGCAGGCCGAAATGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCTGCGATGCGATCATCGCCATGGGGTATGaa < 2:807945/138‑1 (MQ=255) gcagttCGGCCTGCGGCAGGCCGAAATGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCTGCGATGCGATCATCGCCATGGGGTATGaa > 1:807945/5‑142 (MQ=255) aTTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCTGCGATGCGATCATCGCCATGGGGTATGAAGCCGGTGGcc > 1:631964/1‑150 (MQ=255) tCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCTGCGATGCGATCATCGCCATGGGGTATGAAGCCGGTGGCCATc < 2:452632/150‑1 (MQ=255) cTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCTGCGATGCGATCATCGCCATGGGGTATGAAGCCGGTGGCCATCGCGGc > 2:392281/1‑150 (MQ=255) tGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCTGCGATGCGATCATCGCCATGGGGTATGAAGCCGGTGGCCATCGCGGCa < 2:298074/150‑1 (MQ=255) | AGCCTTATTACAGCGAAGTGGGCGCCGATTTCACGGCGCCCACGCCGGTGTCCAACCGTGCGCCTTTCGACGAGCAGAGCTGCCTGCTGGTCGAAGCGTTGCGCCCGGAGGTGGTGAGCTTCCATTTCGGCCTGCCGCAGGCCGAACTGCTGCAGCGGGTAAAGGCCAGCGGTGCCAAGGTACTGTCCAGCGCCACGACCGTGGAAGAAGCGGCCTGGCTGGAGCGCAATGGCTGCGATGCGATCATCGCCATGGGGTATGAAGCCGGTGGCCATCGCGGCA > NC_002947/4353205‑4353486 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |