| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,357,791 | G→A | 9.8% | intergenic (+93/‑161) | PP_3831 → / → PP_3832 | hypothetical protein/CsrA‑like carbon storage regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,357,791 | 0 | G | A | 9.8% | 102.5 / 3.1 | 41 | intergenic (+93/‑161) | PP_3831/PP_3832 | hypothetical protein/CsrA‑like carbon storage regulator |
| Reads supporting (aligned to +/- strand): ref base G (20/17); new base A (2/2); total (22/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01 | |||||||||||
AGTGGCCTTGCTGTTGTTTCTTCAGGCACTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTAGCCCAGCGGACTTATA > NC_002947/4357647‑4357932 | aGTGGCCTTGCTGTTGTTTCTTCAGGCACTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTCAACGGt < 2:112858/150‑1 (MQ=255) cTTGCTGTTGTTTCTTCAGGCACTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCtt < 1:228127/150‑1 (MQ=255) tGCTGTTGTTTCTTCAGGCACTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTg < 2:342763/150‑1 (MQ=255) tttCTTCAGGCACTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGc > 2:59287/1‑150 (MQ=255) aGGCACTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATCCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCCCCTTCCACTGaa > 2:192775/1‑150 (MQ=255) aCTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTCAACGGTTTgt > 2:203770/1‑126 (MQ=255) aCTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTCAACGGTTTgt < 1:203770/127‑2 (MQ=255) ttGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACg > 1:637847/1‑150 (MQ=255) ggaggaCCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGCAGACACGCAACAGAAtt > 2:155198/1‑150 (MQ=255) tATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTa > 2:771028/1‑150 (MQ=255) tATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTa > 2:537902/1‑150 (MQ=255) aTTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCAc > 2:801894/1‑102 (MQ=255) aTTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCAc < 1:801894/102‑1 (MQ=255) aTTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACg < 2:413693/119‑1 (MQ=255) aTTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACg > 1:413693/1‑119 (MQ=255) aTTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTAt > 2:629281/1‑150 (MQ=255) ttCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGa > 2:256919/1‑114 (MQ=255) ttCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGa < 1:256919/114‑1 (MQ=255) tCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATcc > 1:156289/1‑150 (MQ=255) atgCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGATCACCTTTGCCGACACACCGTTGACGGTTTTCTTTGGCACCTGCAACTAAAGACCCGCAACAGAATTTGTCTGTCGGGGAATTAATATCCTCCGAAAGcc > 2:66788/1‑149 (MQ=255) gaagaaATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCtt < 1:276094/150‑1 (MQ=255) gaaATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTaa < 1:516909/150‑1 (MQ=255) aaaTTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAAt < 1:629281/150‑1 (MQ=255) tttCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATgag < 2:156289/150‑1 (MQ=255) aTTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCAc > 2:150853/1‑73 (MQ=255) aTTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCAc < 1:150853/73‑1 (MQ=255) ttAGCCGCCAACTCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTa < 1:624510/116‑1 (MQ=255) ttAGCCGCCAACTCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTa > 2:624510/1‑116 (MQ=255) ttAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAgaaggaag > 2:42395/1‑150 (MQ=255) ttAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAgaaggaag > 1:456245/1‑150 (MQ=255) aGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAagag < 1:537902/150‑1 (MQ=255) cAACCCCCCTATTCTGGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACaa < 1:155198/150‑1 (MQ=255) cccTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTtgtct > 2:458531/1‑90 (MQ=255) cccTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTtgtct < 1:458531/90‑1 (MQ=255) cccTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTccc > 2:73960/1‑150 (MQ=255) cccTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACACAATTTGTCTGTCGGGGAATTACTATCCGACGCAAACGTCCTTAATCAGGAGGAAGAGGGCCAGGCcccc > 1:252419/1‑150 (MQ=255) cAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCa > 2:599536/1‑150 (MQ=255) gAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTa < 2:456245/150‑1 (MQ=255) cACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTAGcc < 1:59287/150‑1 (MQ=255) aagcAAACCGTCAACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTAGCCCAGCGGACt > 2:527654/4‑150 (MQ=255) aaaCCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTAGCCCAGCGGACTtata < 1:42395/150‑1 (MQ=255) | AGTGGCCTTGCTGTTGTTTCTTCAGGCACTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTAGCCCAGCGGACTTATA > NC_002947/4357647‑4357932 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |