Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,437,780 C→T 17.6% A299V (GCG→GTG)  PP_3932 → diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,437,7800CT17.6% 72.0 / 9.3 34A299V (GCG→GTG) PP_3932diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (19/9);  new base T (3/3);  total (22/12)
Fisher's exact test for biased strand distribution p-value = 6.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

AAACCTCATGCACTTCAGCGTGCCGCAGAGCGAGCCTGGGGTTACCACGCTGATGTCGGCGCGCCTGGGCATCGCCATGCTGGTGCTCGGGCCGCTGGTGGTGGCCTGCGTCAGCCAGGCCAACCGTAGCCTCATGGCCCGCCTGGCGCACCAGGCCACCGTCGACCACCTCACCGGTGTGCTCACCCGCAGCGCCTTCACACGCCGCGCCAACGCGCTGCTGGAGAGCCGTCAACAGCACGCCCAGGCGCTGCCGCTGACCCTGATGATGCTGGACATCGACCACT  >  NC_002947/4437634‑4437920
                                                                                                                                                  |                                                                                                                                            
aaaCCTCATGCACTTCAGCGTGCCGCAGAGCGAGCCTGGGGTTACCACGCTGATGTCGGCGCGCCTGGGCATCGCCATGCTGGTGCTCGGGCCGCTGGTGTTGGCCTGCGTCAGCCAGGCCAACCGTAGCCTCATGGCCCGCCTGgcgcc                                                                                                                                           >  2:734515/1‑149 (MQ=255)
    cTCATGCACTTCAGCGTGCCGCAGAGCGAGCCTGGGGTTACCACGCTGATGTCGGCGCGCCTGGGCATCGCCATGCTGGTGCTCGGGCCGCTGGTGGTGGCCTGCGTCAGCCAGGCCAACCGTAGCCTCATGGCCCGCCTGGTGCGCCAg                                                                                                                                       <  1:29236/150‑1 (MQ=255)
     tCATGCACTTCAGCGTGCCGCAGAGCGAGCCTGGGGTTACCACGCTGATGTCGGCGCGCCTGGGCATCGCCATGCTGGTGCTCGGGCCGCTGGTCGGGGCCTGCGTCAGCCAGGCCAACCGTAGCCTCATGGCCCGCCTGGCGCACCAgg                                                                                                                                      >  2:754853/1‑150 (MQ=255)
                   gTGCCGCAGAGCGAGCCTGGGGTTACCACGCTGATGTCGGCGCGCCTGGGCATCGCCATGCTGGTGCTCGGGCCGCTGGTGGTGGCCTGCGTCAGCCAGGCCAACCGTAGCCTCATGGCCCGCCTGGCGCACCAGGCCACCGTCGaccac                                                                                                                        <  1:18603/150‑1 (MQ=255)
                      ccGCAGAGCGAGCCTGGGGTTACCACGCTGATGTCGGCGCGCCTGGGCATCGCCATGCTGGTGCTCGGGCCGCTGGTGGTGGCCTGCGTCAGCCAGGCCAACCGTAGCCTCATGGCCCGCCTGGCGCACCAGGCCACCGTCGACCACCTc                                                                                                                     >  1:412763/1‑150 (MQ=255)
                       cGCAGAGCGAGCCTGGGGTTACCACGCTGATGTCGGCGCGCCTGGGCATCGCCATGCTGGTGCTCGGGCCGCTGGTGGTGGCCTGCGTCAGCCAGGCCAACCTTAGCCTCATGGCCCGCCTGGCGCACCAGGCCACCGTCGACCACCTCa                                                                                                                    >  1:52197/1‑150 (MQ=255)
                                     ggggTTACCACGCTGATGTCGGCGCGCCTGGGCATCGCCATGCTGGTGCTCGGGCCGCTGGTGGTGGCCTGCGTCAGCCAGGCCAACCGTAGCCTCATGGCCCGCCTGGCGCACCAGGCCACCGTCGACCACCTCACCGGTGTGCTCa                                                                                                        <  1:827175/148‑1 (MQ=255)
                                     ggggTTACCACGCTGATGTCGGCGCGCCTGGGCATCGCCATGCTGGTGCTCGGGCCGCTGGTGGTGGCCTGCGTCAGCCAGGCCAACCGTAGCCTCATGGCCCGCCTGGCGCACCAGGCCACCGTCGACCACCTCACCGGTGTGCTCa                                                                                                        >  2:827175/1‑148 (MQ=255)
                                       ggTTACCACGCTGATGTCGGCGCGCCTGGGCATCGCCATGCTGGTGCTCGGGCCGCTGGTGGTGGCCTGCGTCAGCCAGGCCAACCGTAGCCTCATGGCCCGCCTGGCGCACCAGGCCACCGTCGACCACCTCACCGGTGTGCTCACCCg                                                                                                    >  1:49072/1‑150 (MQ=255)
                                             cACGCTGATGTCGGCGCGCCTGGGCATCGCCATGCTGGTGCTCGGGCCGCTGGTGGTGGCCTGCGTCAGCCAGGCCAACCGTAGCCTCATGGCCCGCCTGGCGCACCAGGCCACCGTCGACCACCTCACCGGTGTGCTCACCCGCAgcgc                                                                                              >  1:169172/1‑150 (MQ=255)
                                             cACGCTGATGTCGGCGCGCCTGGGCATCGCCATGCTGGTGCTCGGGCCGCTGGTGGTGGCCTGCGTCAGCCAGGCCAACCGTAGCCTCATGGCCCGCCTGGCGCACCAGGCCACCGTCGACCACCTCACCGGTGTGCTCACCCGCAgcgc                                                                                              >  2:103713/1‑150 (MQ=255)
                                             cACGCTGATGTCGGCGCGCCTGGGCATCGCCATGCTGGTGCTCGGGCCGCTGGTGGTGGCCTGCGTCAGCCAGGCCAACCGTAGCCTCATGGCCCGCCTGGCGCACCAGGCCACCGTCGACCACCTCACCGGTGTGCTCACCCGCAgcgc                                                                                              >  1:73113/1‑150 (MQ=255)
                                                                cTGGGCATCGCCATGCTGGTGCTCGGGCCGCTGGTGGTGGCCTGCGTCAGCCAGGCCAACCGTAGCCCCATGGCCCGCCTGGCGCACCAGGCCACCGTCGGCCACCTCACCGgtg                                                                                                              >  1:747480/1‑115 (MQ=255)
                                                                cTGGGCATCGCCATGCTGGTGCTCGGGCCGCTGGTGGTGGCCTGCGTCAGCCAGGCCAACCGTAGCCCCATGGCCCGCCTGGCGCACCAGGCCACCGTCGACCACCTCACCGgtg                                                                                                              <  2:747480/115‑1 (MQ=255)
                                                                 tGGGCATCGCCATGCTGGTGCTCGGGCCGCTGGTGGTGGCCTGCGTCAGCCAGGCCAACCGTAGCCTCATGGCCCGCCTGGCGCACCAGGCCACCGTCGACCACCTCACCGGTGTGCTCACCCGCAGCGCCTTCACACGCCGCGCCAAcg                                                                          >  2:172374/1‑150 (MQ=255)
                                                                   ggCATCGCCATGCTGGTGCTCGGGCCGCTGGTGGTGGCCTGCGTCAGCCAGGCCAACCGTAGCCTCATGGCCCGCCTGGCGCACCAGGCCACCGTCGACCGCCTCACCGGTGTGCTCACCCGCAGCGCCTTCACACGCCGCGCCAAcgcg                                                                        >  1:746461/1‑150 (MQ=255)
                                                                        cGCCATGCTGGTGCTCGGGCCGCTGGTGGTGGCCTGCGTCAGCCAGGCCAACCGTAGCCTCATGGCCCGCCTGGCGCACCAGGCCACCGTCGACCACCTCACCGGTGTGCTCACCCGCAGCGCCTTCACACGCCGCGCCAACGCgctgct                                                                   <  2:517962/150‑1 (MQ=255)
                                                                             tGCTGGTGCTCGGGCCGCTGGTGGTGGCCTGCGTCAGCCAGGCCAACCGTAGCCTCATGGCCCGCCTGGCGCACCAGGCCACCGTCGACCACCTCACCGGTGTGCTCACCCGCAGCGCCTTCACACGCCGCGCCAACGCGCTGCTGgaga                                                              <  2:412763/150‑1 (MQ=255)
                                                                                tGGTGCTCGGGCCGCTGGTGGTGGCCTGCGTCAGCCAGGCCAACCGTAGCCTCATGGCCCGCCTGGCGCACCAGGCCACCGTCGACCACCTCACCGGTGTGCTCACCCGCAGCGCCTTCACACGCCGCGCCAACGCGCTGCTGgaga                                                              <  1:771555/147‑1 (MQ=255)
                                                                                tGGTGCTCGGGCCGCTGGTGGTGGCCTGCGTCAGCCAGGCCAACCGTAGCCTCATGGCCCGCCTGGCGCACCAGGCCACCGTCGACCACCTCACCGGTGTGCTCACCCGCAGCGCCTTCACACGCCGCGCCAACGCGCTGCTGgaga                                                              >  2:771555/1‑147 (MQ=255)
                                                                                  gTGCTCGGGCCGCTGGTGGTGGCCTGCGTCAGCCAGGCCAACCGTAGCCTCATGGCCCGCCTGGCGCACCAGGCCACCGTCGACCACCTCACCGGTGTGCTCACCCGCAGCGCCTTCACACGCCGCGCCAACGCGCTGCTGGAGAGCCGt                                                         >  1:415716/1‑150 (MQ=255)
                                                                                            cGCTGGTGGTGGCCTGCGTCAGCCAGGCCAACCGTAGCCTCATGGCCCGCCTGGCGCACCAGGCCACCGACGACCACCTCACCGGTATGCTCACCCGCAGCGCCTTCACACGCCGCGCCAACGCGCTGCTGGAGGGCCGTCAACCGCAcc                                               >  2:646719/1‑149 (MQ=255)
                                                                                                   ggtggCCTGCGTCAGCCAGGCCAACCGTAGCCTCATGGCCCGCCTGGCGCACCAGGCCACCGTCGACCACCTCACCGGTGTGCTCACCCGCAGCGCCTTCACACGCCGCGCCAACGCGCTGCTGGAGAGCCGTCAACAGCACGCCCAGgc                                        >  2:696989/1‑150 (MQ=255)
                                                                                                        ccTGCGTCAGCCAGGCCAACCGTAGCCTCATGGCCCGCCTGGCGCACCAGGCCACCGTCGACCACCTCACCGGTGTGCTCACCCGCAGCGCCTTCACACGCCGCGCCAACGCGCTGCTGGAGAGCCGTCAACa                                                    >  2:457156/1‑133 (MQ=255)
                                                                                                        ccTGCGTCAGCCAGGCCAACCGTAGCCTCATGGCCCGCCTGGCGCACCAGGCCACCGTCGACCACCTCACCGGTGTGCTCACCCGCAGCGCCTTCACACGCCGCGCCAACGCGCTGCTGGAGAGCCGTCAACa                                                    <  1:457156/133‑1 (MQ=255)
                                                                                                                         aaCCGTAGCCTCATGGCCCGCCTGGCGCACCAGGCCACCGTCGACCACCTCACCg                                                                                                                 <  2:597584/55‑1 (MQ=255)
                                                                                                                         aaCCGTAGCCTCATGGCCCGCCTGGCGCACCAGGCCACCGTCGACCACCTCACCg                                                                                                                 >  1:597584/1‑55 (MQ=255)
                                                                                                                             gTAGCCTCATGGCCCGCCTGGCGCACCAGGCCACCGTCGACCACCTCACCGGTGTGCTCACCCGCAGCGCCTTCACACGCCGCGCCAACGCGCTGCTGGAGAGCCGTCAACAGCACGCCCAGGCGCTGCCGCTGACCCTGATGATGCTgg              >  1:131002/1‑150 (MQ=255)
                                                                                                                                        ggtggCCTGGTGCGCCAGGCCACCGTCGACCACCTCACCGGTGTGCTCACCCGCAGCGCCTTCACACGCCGCGCCAACGCGCTGCTGGAGAGCCGTCAACAGCACGCCCAGGCGCTGCCGCTGACCCtgat                      >  1:47687/5‑131 (MQ=255)
                                                                                                                                        ggtggCCTGGTGCGCCAGGCCACCGTCGACCACCTCACCGGTGTGCTCACCCGCAGCGCCTTCACACGCCGCGCCAACGCGCTGCTGGAGAGCCGTCAACAGCACGCCCAGGCGCTGCCGCTGACCCtgat                      <  2:47687/127‑1 (MQ=255)
                                                                                                                                         gtggCCTGGTGCGCCAGGCCACCGTCGACCACCTCACCGGTGTGCTCACCCGCAGCGCCTTCACACGCCGCGCCAACGCGCTGCTGGAGAGCCGTCAACAGCACGCCCAGGCGCTGCCGCTGACCCTGATGATGCTGGACACCGACCACt  >  2:70870/4‑150 (MQ=255)
                                                                                                                                          ccGCCTGGCGCACCAGGCCACCGTCGACCACCTCACCGGTGTGCTCACCCGCAGCGCCTTCACACGCCGCGCCAACGCGCTGCTGGAGAGCCGTCAACAGCACGccc                                            >  1:4244/1‑107 (MQ=255)
                                                                                                                                          ccGCCTGGCGCACCAGGCCACCGTCGACCACCTCACCGGTGTGCTCACCCGCAGCGCCTTCACACGCCGCGCCAACGCGCTGCTGGAGAGCCGTCAACAGCACGccc                                            <  2:4244/107‑1 (MQ=255)
                                                                                                                                            gCCTGGTGCGCCAGGCCACCGTCGACCACCTCACCGGTGTGCTCACCCGCAGCGCCTTCACACGCCGCGCCAACGCGCTGCTGGAGAGCCGTCAACAGCACGCCCAGGCGCTGCTGCTGACCCTGATGATGCTGGACATCGAc      >  2:626726/1‑143 (MQ=255)
                                                                                                                                            gCCTGGTGCGCCAGGCCACCGTCGACCACCTCACCGGTGTGCTCACCCGCAGCGCCTTCACACGCCGCGCCAACGCGCTGCTGGAGAGCCGTCAACAGCACGCCCAGGCGCTGCCGCTGACCCTGATGATGCTGGACATCGAc      <  1:626726/143‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
AAACCTCATGCACTTCAGCGTGCCGCAGAGCGAGCCTGGGGTTACCACGCTGATGTCGGCGCGCCTGGGCATCGCCATGCTGGTGCTCGGGCCGCTGGTGGTGGCCTGCGTCAGCCAGGCCAACCGTAGCCTCATGGCCCGCCTGGCGCACCAGGCCACCGTCGACCACCTCACCGGTGTGCTCACCCGCAGCGCCTTCACACGCCGCGCCAACGCGCTGCTGGAGAGCCGTCAACAGCACGCCCAGGCGCTGCCGCTGACCCTGATGATGCTGGACATCGACCACT  >  NC_002947/4437634‑4437920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑