Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,470,272 A→C 14.9% V321G (GTG→GGG)  PP_3961 ← transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,470,2720AC14.9% 72.1 / 6.3 32V321G (GTG→GGG) PP_3961transporter
Reads supporting (aligned to +/- strand):  ref base A (11/16);  new base C (2/3);  total (13/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAATCCGGCCTGGGCACCCCACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCGGGGTATCG  >  NC_002947/4470124‑4470415
                                                                                                                                                    |                                                                                                                                               
gAATCCGGCCTGGGCACCCCACACATCGACCACCTGCCCAGATGCTGCGGCGCCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCAc                                                                                                                                                <  2:826790/150‑1 (MQ=255)
  aTCCGGCCTGGGCACCCCACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGa                                                                                                                                              >  1:335259/1‑150 (MQ=255)
   tCCGGCCTGGGCACACAAAAACTCAACCACATCCCAAGATTATTCTGCACCGCCTGTAACTCCAACGTTGATACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGAt                                                                                                                                             >  2:737613/1‑150 (MQ=255)
      ggCCTGGGCACCCCACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCCCGACCAt                                                                                                                                          >  1:439277/1‑150 (MQ=255)
          tGGGCACCCCACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGCTCATGGTc                                                                                                                                      <  2:426646/150‑1 (MQ=255)
          tGGGCACCCCACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTc                                                                                                                                      <  2:837647/150‑1 (MQ=255)
             gCACCCCACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCggg                                                                                                                                   <  1:106445/150‑1 (MQ=255)
                  ccACACATCGACCACCTGCCCCGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCCCCACCATGATCGTGGCgat                                                                                                                              <  2:439277/150‑2 (MQ=255)
                  ccACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCCCGACCATGATCGTGGCgat                                                                                                                              <  2:87360/150‑2 (MQ=255)
                  ccACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCgaa                                                                                                                              <  2:804724/150‑1 (MQ=255)
                                   gCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCa                                                                                                             <  2:468277/150‑1 (MQ=255)
                                     ccAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGc                                                                                                           >  1:635195/1‑150 (MQ=255)
                                             tgcGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGAt                                                                                                   <  2:826365/150‑1 (MQ=255)
                                               cGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATgg                                                                                                 <  2:635195/150‑1 (MQ=255)
                                                        cgGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCga                                                                                        >  2:643187/1‑150 (MQ=255)
                                                               tCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCCCGACCATGATCGTGGCgat                                                                                                                              <  2:218225/105‑2 (MQ=255)
                                                               tCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCCCGACCATGATCGTGGCgat                                                                                                                              >  1:218225/1‑104 (MQ=255)
                                                                  aaCGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTcc                                                                              <  1:486570/150‑1 (MQ=255)
                                                                                       ccAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATCCCCGGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAgtt                                                         >  1:8211/1‑148 (MQ=255)
                                                                                            tCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTcg                                                    <  2:671641/150‑1 (MQ=255)
                                                                                                                 tttCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAg                                                           <  2:366326/122‑1 (MQ=255)
                                                                                                                 tttCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAg                                                           >  1:366326/1‑122 (MQ=255)
                                                                                                                             tGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTag                        >  2:173587/1‑145 (MQ=255)
                                                                                                                             tGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTag                        <  1:173587/145‑1 (MQ=255)
                                                                                                                                 ctctACAAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCCCCACCGGCAAGGAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTtgtg               <  2:335259/150‑1 (MQ=255)
                                                                                                                                      ccAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTATCCCACCACCAGCAGCCGAACGGTGGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCg          >  2:370923/1‑150 (MQ=255)
                                                                                                                                        aGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCggg        <  2:610955/150‑1 (MQ=255)
                                                                                                                                        aGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCggg        <  1:737613/150‑1 (MQ=255)
                                                                                                                                           gACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCCGCCCAATGTTGTTTCACACCGGGATTTTTGGCAAGTTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTCGCAGGGTTGGTAGGGGGGTa     >  1:700659/1‑150 (MQ=255)
                                                                                                                                              aTCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCGGGGTATCg  >  1:800432/1‑150 (MQ=255)
                                                                                                                                               tCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGttt                                                                                <  2:500388/71‑1 (MQ=255)
                                                                                                                                               tCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGttt                                                                                >  1:500388/1‑71 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GAATCCGGCCTGGGCACCCCACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCGGGGTATCG  >  NC_002947/4470124‑4470415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑