Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,470,276 T→C 16.1% I320V (ATC→GTC)  PP_3961 ← transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,470,2760TC16.1% 66.3 / 6.5 31I320V (ATC→GTC) PP_3961transporter
Reads supporting (aligned to +/- strand):  ref base T (10/16);  new base C (2/3);  total (12/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.20e-01

TCCGGCCTGGGCACCCCACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCGGGGTATCGAGTTTCAG  >  NC_002947/4470127‑4470423
                                                                                                                                                     |                                                                                                                                                   
tCCGGCCTGGGCACACAAAAACTCAACCACATCCCAAGATTATTCTGCACCGCCTGTAACTCCAACGTTGATACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGAt                                                                                                                                                     >  2:737613/1‑150 (MQ=255)
   ggCCTGGGCACCCCACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCCCGACCAt                                                                                                                                                  >  1:439277/1‑150 (MQ=255)
       tGGGCACCCCACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGCTCATGGTc                                                                                                                                              <  2:426646/150‑1 (MQ=255)
       tGGGCACCCCACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTc                                                                                                                                              <  2:837647/150‑1 (MQ=255)
          gCACCCCACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCggg                                                                                                                                           <  1:106445/150‑1 (MQ=255)
               ccACACATCGACCACCTGCCCCGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCCCCACCATGATCGTGGCgat                                                                                                                                      <  2:439277/150‑2 (MQ=255)
               ccACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCCCGACCATGATCGTGGCgat                                                                                                                                      <  2:87360/150‑2 (MQ=255)
               ccACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCgaa                                                                                                                                      <  2:804724/150‑1 (MQ=255)
                                gCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCa                                                                                                                     <  2:468277/150‑1 (MQ=255)
                                  ccAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGc                                                                                                                   >  1:635195/1‑150 (MQ=255)
                                          tgcGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGAt                                                                                                           <  2:826365/150‑1 (MQ=255)
                                            cGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATgg                                                                                                         <  2:635195/150‑1 (MQ=255)
                                                     cgGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCga                                                                                                >  2:643187/1‑150 (MQ=255)
                                                            tCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCCCGACCATGATCGTGGCgat                                                                                                                                      <  2:218225/105‑2 (MQ=255)
                                                            tCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCCCGACCATGATCGTGGCgat                                                                                                                                      >  1:218225/1‑104 (MQ=255)
                                                               aaCGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTcc                                                                                      <  1:486570/150‑1 (MQ=255)
                                                                                    ccAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATCCCCGGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAgtt                                                                 >  1:8211/1‑148 (MQ=255)
                                                                                         tCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTcg                                                            <  2:671641/150‑1 (MQ=255)
                                                                                                              tttCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAg                                                                   >  1:366326/1‑122 (MQ=255)
                                                                                                              tttCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAg                                                                   <  2:366326/122‑1 (MQ=255)
                                                                                                                          tGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTag                                <  1:173587/145‑1 (MQ=255)
                                                                                                                          tGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTag                                >  2:173587/1‑145 (MQ=255)
                                                                                                                              ctctACAAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCCCCACCGGCAAGGAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTtgtg                       <  2:335259/150‑1 (MQ=255)
                                                                                                                                   ccAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTATCCCACCACCAGCAGCCGAACGGTGGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCg                  >  2:370923/1‑150 (MQ=255)
                                                                                                                                     aGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCggg                <  2:610955/150‑1 (MQ=255)
                                                                                                                                     aGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCggg                <  1:737613/150‑1 (MQ=255)
                                                                                                                                        gACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCCGCCCAATGTTGTTTCACACCGGGATTTTTGGCAAGTTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTCGCAGGGTTGGTAGGGGGGTa             >  1:700659/1‑150 (MQ=255)
                                                                                                                                           aTCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCGGGGTATCg          >  1:800432/1‑150 (MQ=255)
                                                                                                                                            tCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGttt                                                                                        <  2:500388/71‑1 (MQ=255)
                                                                                                                                            tCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGttt                                                                                        >  1:500388/1‑71 (MQ=255)
                                                                                                                                                  cGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCGGGGTATCGAGTTTcc   >  2:217158/1‑149 (MQ=255)
                                                                                                                                                   gATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAGCCCACCCAGCAGTAGCAGCTTGTGTAGCGGGGTATCGAGTTTCAg  <  1:788143/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TCCGGCCTGGGCACCCCACACATCGACCACCTGCCCAGATGCTGCGGCACCGCCGGCAACTCCAACGTTGAGACCGGCGAGCAACCAGGTCATCCCTTCGGTCAGCTTGCTTTCGGGCACGATGCGCTCTACCAGCGACATCGCCACGATCATGGTCGGGGCGAAGAACAGGCCCGCAACCAGCACGGCGATGGCCAGCCCGATGATGTTTCCCACCACCAGCAGCGGCAAGGTTGTCGCCGCCGTCGCCAACCCACCCAGCAGTAGCAGCTTGTGTAGCGGGGTATCGAGTTTCAG  >  NC_002947/4470127‑4470423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑