| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,522,021 | G→T | 12.2% | intergenic (‑240/‑175) | icd ← / → idh | NADP(+)‑specific isocitrate dehydrogenase/isocitrate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,522,021 | 0 | G | T | 12.2% | 115.6 / 5.4 | 41 | intergenic (‑240/‑175) | icd/idh | NADP(+)‑specific isocitrate dehydrogenase/isocitrate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (18/18); new base T (2/3); total (20/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
TTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGATGCATCCACCATCACCGCTCGCAGCCTCTCGACTTTCCGCTCATGGCGCTGCCAGGGCGATGCGCCTACCCTGCGCACCACGAGACTCGA > NC_002947/4521882‑4522165 | ttGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATTCATGGcctag < 1:446518/150‑4 (MQ=255) tgctgcTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATTCATGGcctagg < 1:106697/150‑5 (MQ=255) ctgctTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAg < 2:809882/150‑1 (MQ=255) tgctTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGc < 1:505457/150‑1 (MQ=255) ttAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATGAGCCCAAATGGTTTGGTATACTCCCTCCCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTg < 1:274734/150‑1 (MQ=255) ttATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGgttgt < 2:92466/143‑1 (MQ=255) ttATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGgttgt > 1:92466/1‑143 (MQ=255) gCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTggg > 2:730193/1‑129 (MQ=255) gCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTggg < 1:730193/129‑1 (MQ=255) aCTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGgtt > 2:496173/1‑122 (MQ=255) aCTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGgtt < 1:496173/122‑1 (MQ=255) ccTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATTCATGGcctagg > 2:642582/1‑102 (MQ=255) ccTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATTCATGGcctagg < 1:642582/106‑5 (MQ=255) ccTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGAt < 2:625050/150‑1 (MQ=255) cTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGATg > 1:719618/1‑150 (MQ=255) ttGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTg > 2:78096/1‑135 (MQ=255) ttGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTg < 1:78096/135‑1 (MQ=255) gATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGATGCATcca > 2:628342/1‑150 (MQ=255) ccAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGATGCATCCACCATCAc > 2:551407/1‑150 (MQ=255) aaaTGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGAt < 1:155996/134‑1 (MQ=255) aaaTGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGAt > 2:155996/1‑134 (MQ=255) aaTGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGATGCATCCACCATCACCGc > 1:329660/1‑150 (MQ=255) gTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGATGCATCCACCATCACCGCTCGCAGCct > 1:263524/1‑150 (MQ=255) cTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGATGCATCCACCATCACCGCTCGCAGCCTCTCGa < 1:551407/150‑1 (MQ=255) tCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGATGCATCCACCATCACCGCTCGCAGCCTCTCGAc > 1:121353/1‑150 (MQ=255) ccgccgCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGATGCa < 1:208094/120‑1 (MQ=255) ccgccgCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGATGCa > 2:208094/1‑120 (MQ=255) cGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGATGCATCCACCATCACCGCTCGCAGCCTCTCGACTTTCCGCTCAt < 2:263524/150‑1 (MQ=255) gAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCt > 2:702614/1‑98 (MQ=255) gAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCt < 1:702614/98‑1 (MQ=255) aTCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGATGCATCCACCATCACCGCTCGCAGCCTCTCGACTTTCCGCTCATGGCGCTGcc > 1:449047/1‑150 (MQ=255) ggggCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGATGCATCCACCATCACCGCTCGCAGCCTCTCGACTTTCCGCTCATGGCGCTGCCAgg < 1:628342/150‑1 (MQ=255) gAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGATGCATCCACCATCACCGCTCGCAGCCTCTCGACTTTCCGCTCATCGCGCTGCCAGGGCGAt > 2:477828/1‑150 (MQ=255) ggACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGATGCATCCACCATCACCGCTCGCAGCCTATCGACTTTCCGCTCATGGCGCTGCCAGGGCGATGCGCCTACCCTGCGcac > 2:82212/1‑150 (MQ=255) gACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGATGCATCCACCATCACCGCTCGCAGCCTCTCGACTTTc < 2:21423/108‑1 (MQ=255) gACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGATGCATCCACCATCACCGCTCGCAGCCTCTCGACTTTc > 1:21423/1‑108 (MQ=255) gCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGATGCATCCACCATCACCGCTCGCAGCCTCTCGACTTTCCGCTCATGGCGCTGCCAGGGCGATGCGCCTACCCTGCGCACCACga < 1:477828/150‑1 (MQ=255) tttatggCCATTCATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGATGCATCCACCATCACCGCTCGCAGCCTCTCGACTTTCCGCTCATGGCGCTGCCAGGGCGATGCGCCTACCCTGCGCACCACgag > 2:13629/6‑150 (MQ=255) gCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGATGCATCCACCATCACCGCTCGCAGCCTCTCGACTTTCCGCTCATGGCGCTGCCAGGGCGATGCGCCTACCCTGCGCACCACGAGACTCGa < 2:470622/150‑1 (MQ=255) aTGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGATGCATCCACCATCACCGCTCGCAGCCTCTCGACTTTCCGCTCATGGCGCTGCCAGGGCGATgcgc > 2:568589/1‑122 (MQ=255) aTGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGATGCATCCACCATCACCGCTCGCAGCCTCTCGACTTTCCGCTCATGGCGCTGCCAGGGCGATgcgc < 1:568589/122‑1 (MQ=255) | TTGCTGCTTAGTCTTTGGTCTTATAAATGGGCCGATATCACTTGGCCTTGCTGATTAGCCCAAATGGTTTGGTATACTCCGCCGCGACCGAAGGGTCATCGGGGCGAATCCCAGGAAAATGCGGACCGCCCTAGGCCATGAATGGCCATAAAGCCTGGGTTGTTACCGCAGCTCAGCACTTGACGCTCGACTGATGCATCCACCATCACCGCTCGCAGCCTCTCGACTTTCCGCTCATGGCGCTGCCAGGGCGATGCGCCTACCCTGCGCACCACGAGACTCGA > NC_002947/4521882‑4522165 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |