| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,528,397 | T→A | 10.9% | intergenic (+28/‑48) | purB → / → PP_4017 | adenylosuccinate lyase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,528,397 | 0 | T | A | 10.9% | 128.5 / 7.9 | 46 | intergenic (+28/‑48) | purB/PP_4017 | adenylosuccinate lyase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (17/24); new base A (2/3); total (19/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.66e-01 | |||||||||||
CAGAAGCGCTGCTGACCTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCGCTGCTGGTACGCCAG > NC_002947/4528248‑4528546 | cAGAAGCGCTGCTGACCTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCt > 1:118065/1‑150 (MQ=255) tgctgACCTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCAGGCCGGGCg < 1:135939/150‑1 (MQ=255) tgACCTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGGCGCGCAACCTGACCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTAAAACGCCCGGCCAGGCCGGGCGttg < 2:450959/150‑2 (MQ=255) cTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGc > 1:767552/1‑137 (MQ=255) cTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGc < 2:767552/137‑1 (MQ=255) cTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTAt > 2:539660/1‑150 (MQ=255) tCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTc < 2:409479/150‑1 (MQ=255) aTCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCTGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTccc < 2:627844/150‑1 (MQ=255) cTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGaaaa > 2:78725/1‑150 (MQ=255) tcgACATGCCACCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGTCCAAACTCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGCGCGTTTTTATTCCCGTACGAAAAt < 2:234456/148‑1 (MQ=255) cAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGttttt < 2:357794/150‑1 (MQ=255) aGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGtttttt < 2:529341/150‑1 (MQ=255) ccAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGt < 1:529264/150‑1 (MQ=255) ccAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGt < 2:173007/150‑1 (MQ=255) aaGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAAt < 2:587855/144‑1 (MQ=255) aaGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAAt > 1:587855/1‑144 (MQ=255) cGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACAt < 2:472926/150‑1 (MQ=255) gCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATCGGTTGAACATGaa > 2:106797/1‑150 (MQ=255) tCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGtt > 1:163864/1‑101 (MQ=255) tCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGtt < 2:163864/101‑1 (MQ=255) gcagcTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATa > 2:791019/1‑131 (MQ=255) gcagcTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATa < 1:791019/131‑1 (MQ=255) gcagcTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGa > 2:559446/1‑150 (MQ=255) gcagcTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGa < 2:235498/150‑1 (MQ=255) tGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCt > 1:194320/1‑143 (MQ=255) tGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCt < 2:194320/143‑1 (MQ=255) cGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCa > 1:110736/1‑150 (MQ=255) agCTACCTATCTCGGTAACGCGGCCGTCCAGGCCAACCGAATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTCTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCa < 2:193109/149‑1 (MQ=255) gCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAg < 2:110736/150‑1 (MQ=255) cgcgGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCAtctc > 2:471108/1‑150 (MQ=255) gcgGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCg > 1:287995/1‑150 (MQ=255) gcgGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCg < 2:118065/150‑1 (MQ=255) aGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTAAAACGCCCGGCCAGGCCGGGCGttgta < 2:290471/64‑4 (MQ=25) aGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTAAAACGCCCGGCCAGGCCGGGCGttgta > 1:290471/1‑61 (MQ=25) ccAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGa > 2:606576/1‑150 (MQ=255) cAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGaa < 2:287995/150‑1 (MQ=255) aGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTc > 1:318803/1‑63 (MQ=255) aGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTc < 2:318803/63‑1 (MQ=255) aaCGAATCTAAGCTGAGCGTCGCGTACAACGCCACGCCTGGCCCGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATgc < 1:351355/150‑1 (MQ=255) tAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATa < 1:481336/97‑1 (MQ=255) tAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATa > 2:481336/1‑97 (MQ=255) cTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGc < 1:606576/150‑1 (MQ=255) gcgTACAACGCCCGGCCTGGCCGGGCGTTTTTATTGCCGGACGAGAATGACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGTTCGGCCGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGc > 2:590508/1‑150 (MQ=255) gcgTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTAAGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCGCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGc < 2:143446/150‑1 (MQ=255) cgTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCAGTGTGTTCATGCAGGCCAGCTTGCAGAAGAAGcc > 2:185520/1‑150 (MQ=255) aCGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCgctgct < 1:471108/150‑1 (MQ=255) aCGCCCGGCCAGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCgctgct > 2:664456/1‑150 (MQ=255) tGGCCGGGCGTTTTTATCCCCGTACGAAATTTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCGGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCGCTGCTGGTACGCCAg < 2:177708/150‑1 (MQ=255) | CAGAAGCGCTGCTGACCTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCGCTGCTGGTACGCCAG > NC_002947/4528248‑4528546 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |