Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,564,768 C→T 13.2% intergenic (‑864/‑34) vgrG‑III ← / → glgA type VI secretion system protein/glycogen synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,564,7680CT13.2% 88.9 / 6.3 38intergenic (‑864/‑34)vgrG‑III/glgAtype VI secretion system protein/glycogen synthase
Reads supporting (aligned to +/- strand):  ref base C (17/16);  new base T (2/3);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

GGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGCTCACCCCGGATTTCGCCACGACAGGCAAGGCACCCGGCGC  >  NC_002947/4564628‑4564902
                                                                                                                                            |                                                                                                                                      
ggCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGa                                                                                                                               >  2:717633/1‑150 (MQ=255)
ggCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGa                                                                                                                               <  1:717633/150‑1 (MQ=255)
   caccaACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCTGGCACCCgtggc                                                                                                                            <  1:303669/150‑5 (MQ=255)
    accaACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCaa                                                                                                                           >  1:287426/1‑150 (MQ=255)
    accaACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCaa                                                                                                                           <  1:764254/150‑1 (MQ=255)
    accaACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCaa                                                                                                                           >  2:502210/1‑150 (MQ=255)
      gtcgccaacAGCCCAATCGCTTTCGGCCGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCAACGGGTGCCAGCACTCGACCAAGc                                                                                                                         >  1:457981/5‑150 (MQ=255)
           caacAGCCCAATCGCTTTCGGCCGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAAAGAACTACGCAGCATTCAGCAAATATACAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAgg                                                                                                                    <  1:744407/150‑1 (MQ=255)
                  ccAATCGCTTTCGGCCGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAATCATAGGGACGgag                                                                                                             <  1:379314/150‑1 (MQ=255)
                    aaTCGCTTTCGGCCGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCTGGCACCCgtg                                                                                                                              <  1:584481/131‑3 (MQ=255)
                    aaTCGCTTTCGGCCGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATAGCCACGGGTGCTGGCACCCgtg                                                                                                                              >  2:584481/1‑129 (MQ=255)
                               gCCGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCa                                                                                                >  2:556098/1‑150 (MQ=255)
                                          gATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTc                                                                                     >  2:316976/1‑150 (MQ=255)
                                            tCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGa                                                                                   <  1:731076/150‑1 (MQ=255)
                                                gcCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCt                                                                               >  2:656333/1‑150 (MQ=255)
                                                 tcgtgcgcgCACGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTc                                                                              >  2:578220/2‑150 (MQ=255)
                                                               aCGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCCGCACCCGACCAAGCATAGAGACTGAGAGAATTATGAGCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTc                                                                <  1:363645/150‑1 (MQ=255)
                                                                   cacaGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAAc                                                            <  1:41592/150‑1 (MQ=255)
                                                                    acaGGGGCATGGACCGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACACCGCCACGGGTCCCAGCACCCGACCAAGCATATGGACGGAGAGAAGCATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAAcc                                                           <  1:286319/150‑1 (MQ=255)
                                                                            aTGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAAcc                                                   >  2:340463/1‑150 (MQ=255)
                                                                              ggAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACcgc                                                 <  1:578220/150‑1 (MQ=255)
                                                                                 aCGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAg                                              <  1:556098/150‑1 (MQ=255)
                                                                                 aCGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGACGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAg                                              >  2:2650/1‑150 (MQ=255)
                                                                                   gCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGcc                                            >  1:753792/1‑150 (MQ=255)
                                                                                     aCTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGGGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGc                                          >  1:481602/1‑150 (MQ=255)
                                                                                       tACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGCTc                                        >  2:439208/1‑150 (MQ=255)
                                                                                          gcagcaTTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGCTCAcc                                     <  2:287426/150‑1 (MQ=255)
                                                                                            agcaTTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGCTCAcccc                                   <  1:491696/150‑1 (MQ=255)
                                                                                               aTTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGCTCACCCCGGa                                <  1:502210/150‑1 (MQ=255)
                                                                                                            agagaACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGCTCACCCCGGATTTCGCCACGACa                   >  2:588831/1‑150 (MQ=255)
                                                                                                                aaCCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGTGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGCTCACCCCGGATTTCGc                          <  1:625195/139‑1 (MQ=255)
                                                                                                                aaCCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGTGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGCTCACCCCGGATTTCGc                          >  2:625195/1‑139 (MQ=255)
                                                                                                                       aCGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGCGAGAAGTATGATCAGAGCCGCTGTCGAGCCTCACGTAGACGCTTTCAACCCGGATAAACGCGAGCCGCTCACCCCGGATTTCGCCACGACaggcaaggcaac        >  1:316051/1‑148 (MQ=255)
                                                                                                                         gAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGCTCACCCCGGATTTCGCCACGACAGGCAAGGCACCCg      >  1:14724/1‑150 (MQ=255)
                                                                                                                           aaCATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGCTCACCCCGGATTTCGCCACGACAGGCAAGGCACCCGgc    <  1:439208/150‑1 (MQ=255)
                                                                                                                             cATCGCCACGGGTGCCAGCACCCGACCAAGCATAGCGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTAGAAGCGGATGACCGCGGGTCGCTTACCCCGGATTTCGCCACGACAGGCAAGGCACCCGgcgc  <  2:457981/150‑1 (MQ=255)
                                                                                                                                    tcGGGTGCTGGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGAc                                                                      >  1:846786/2‑75 (MQ=255)
                                                                                                                                    tcGGGTGCTGGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGAc                                                                      <  2:846786/74‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                      
GGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATCGCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGCCTCACGTAGACGCTTTCAAACCGGATAACCGCGAGCCGCTCACCCCGGATTTCGCCACGACAGGCAAGGCACCCGGCGC  >  NC_002947/4564628‑4564902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑