| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 365,816 | A→C | 9.8% | E27A (GAG→GCG) ‡ | cdhR → | HTH‑type transcriptional regulator CdhR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 365,816 | 0 | A | C | 9.8% | 148.9 / 8.1 | 58 | E27A (GAG→GCG) ‡ | cdhR | HTH‑type transcriptional regulator CdhR |
| Reads supporting (aligned to +/- strand): ref base A (27/25); new base C (4/2); total (31/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01 | |||||||||||
TACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAATGCCGATGCCGCCCTGGGGGAGGGCGAGCCGGCCAACATCCTGCT > NC_002947/365670‑365963 | tacgctGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGcc < 2:565244/150‑1 (MQ=255)tacgctGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGcc > 1:733884/1‑150 (MQ=255) gtgtgcccTTGCCCCAGCGTACGCTAGCGACAGGAAGTAGCACCGGGGCGACAGGTCTCTGAGGTGAACAGCATCACGTAGACGATCGCGTTTCTGTTGATGCCCGGCTTTTCTGCCATGGGCTTCATTTCGGCAGTGGAGCCGCTGCgc > 2:101618/7‑149 (MQ=255) aCCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGc < 2:12444/150‑1 (MQ=255) gctgacccAAGCGCACGCTAGCGACAGGAATTAGCACCGGAGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGcc > 2:737725/6‑150 (MQ=255) ccTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCgg > 1:190527/1‑144 (MQ=255) ccTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCgg < 2:190527/144‑1 (MQ=255) aGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGtttt < 2:297847/150‑1 (MQ=255) gcgcACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTtg > 1:757237/1‑150 (MQ=255) gcgcACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTtg > 1:423398/1‑150 (MQ=255) cgcACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTtgg < 2:733884/150‑1 (MQ=255) gcACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTtggt < 1:438535/150‑1 (MQ=255) aCGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTtggtgg < 2:279061/150‑1 (MQ=255) gCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGc > 2:835067/1‑150 (MQ=255) cTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCa < 2:757237/150‑1 (MQ=255) aCAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTa > 2:554811/1‑150 (MQ=255) ttAGCACCGGGGCGACAAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCGGGCTTTTCTGCCATGGGGTTCATTTCGGCAGGGGAGCCACTGCGGGTGGCCCATCGGTTTGGGGGGCAGTTGTGCCGGTgg > 2:624003/1‑150 (MQ=255) aCCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGt > 2:727312/1‑150 (MQ=255) ggCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGACAATCGTTTTGGTGGGCAGTTGTACCGTGGGCAGGTGCTTa < 2:423398/150‑1 (MQ=255) ggCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCCGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTa < 2:573290/150‑1 (MQ=255) gACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGcc > 1:713685/1‑150 (MQ=255) tgtgGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGc < 2:842024/96‑1 (MQ=255) tgtgGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGc > 1:842024/1‑96 (MQ=255) tgtgGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGa < 2:713685/150‑1 (MQ=255) gaggagGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCg > 2:339554/1‑150 (MQ=255) ggaggGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGt < 1:526835/150‑1 (MQ=255) ggAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCaa < 1:348899/150‑1 (MQ=255) cTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAAt < 2:186917/139‑1 (MQ=255) cTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAAt > 1:186917/1‑139 (MQ=255) tttCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGCGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCTTGGACGGTGAGGCGGTGCTGGCTAGCGATGGCATGTCGATc > 2:258610/1‑150 (MQ=255) ttCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTggg < 1:477972/80‑1 (MQ=255) ttCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTggg > 2:477972/1‑80 (MQ=255) ggggtGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAat < 2:645286/146‑1 (MQ=255) gTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAatgc < 1:835067/150‑1 (MQ=255) gCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAatgccgatgc < 1:554811/150‑1 (MQ=255) ccGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAatgccgatgccg > 1:166151/1‑150 (MQ=255) ccGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAatgccgatgccg > 2:434389/1‑150 (MQ=255) cGGCTTTTCTGCCATGGGGGTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAATGCCGATgccgc > 1:365128/1‑150 (MQ=255) cTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAATGCCGATGCCGCCCt < 1:434389/150‑1 (MQ=255) ttttCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAATGCCGATGCCGCCCTg > 2:209269/1‑150 (MQ=255) tGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAATGCCGATGCCGCCCt > 1:601442/1‑144 (MQ=255) tGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAATGCCGATGCCGCCCt < 2:601442/144‑1 (MQ=255) ccATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAATGCCGATGCCGCCCTGGGGGAgg > 1:736523/1‑150 (MQ=255) ccATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAATGCCGATGCCGCCCTGGGGGAgg < 1:727312/150‑1 (MQ=255) cATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAATGCCGATGCCGCCCTggggg < 2:564451/146‑1 (MQ=255) cATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAATGCCGATGCCGCCCTggggg > 1:564451/1‑146 (MQ=255) cATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAATGCCGATGCCGCCCTGGGGGAggg > 1:647269/1‑150 (MQ=255) ttCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAATGCCGATGCCGCCCTGGGGGAGGGCGAGCCg > 1:625571/1‑150 (MQ=255) ttCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAATGCCGATGCCGCCCTGGGGGAGGGCGAGCCg > 1:150634/1‑150 (MQ=255) ccacccgCAGTGGCTCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAATGCCGATGCCGCCCT‑GGGGAGGGCGAGCCGGCCa > 1:63000/7‑150 (MQ=255) aTTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAATGCCGATGCCGCCCTGGGGGAGGGCGAGCCGGcc > 1:102278/1‑150 (MQ=255) aTTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAATGCCGATGCCGCCCTGGGGGAGGGCGAGCCGGcc < 2:365128/150‑1 (MQ=255) acccgCAGTGGCTCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAATGCCGATGCCGCCCTGGGGGAgg < 2:74693/133‑1 (MQ=255) acccgCAGTGGCTCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAATGCCGATGCCGCCCTGGGGGAgg > 1:74693/5‑137 (MQ=255) ccgCAGTGGCTCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGa > 2:141568/3‑108 (MQ=255) ccgCAGTGGCTCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGa < 1:141568/106‑1 (MQ=255) ggCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAATGCCGATGCCGCCCTGGGGGAGGGCGAGCCGGCCAACAt > 1:599897/1‑150 (MQ=255) ggAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAATGCCGATGCCGCCCTGGGGGAGGGCGAGCCGGCCAACATCctgct < 2:647269/150‑1 (MQ=255) | TACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGTTGTACCGGTGGCAGGTGCTTAGCCTGGACGGTGAGGCGGTGCTGGCGAGCAATGGCATGTCGATCAATGCCGATGCCGCCCTGGGGGAGGGCGAGCCGGCCAACATCCTGCT > NC_002947/365670‑365963 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |