| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,646,078 | G→A | 19.0% | Q199Q (CAG→CAA) | fusB → | elongation factor G 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,646,078 | 0 | G | A | 19.0% | 36.2 / 4.3 | 21 | Q199Q (CAG→CAA) | fusB | elongation factor G 2 |
| Reads supporting (aligned to +/- strand): ref base G (10/7); new base A (2/2); total (12/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01 | |||||||||||
GCCGACTTCCTGCGCGTGGTCAAGCAGATCGATCAACGTCTGGGGCACCACCCGGTGCCCATCCAGCTGGCCATCGGCAGCGAAGAAAACTTCATGGGCCAGATCGACCTGGTGAAGATGAAGGCCATCTACTGGAACGATGCCGACCAGGGCACCAGCTACCGCGAAGAAGAAATCCCCGCCGAACTGAAGGCTCTGGCCGACGAATGGCGCGCGCACATGATCGAAGCTGCGGCCGAGGCCAATGACGAGCTGACCATGAAGTTTCTCGATGGCGAGGAGCTGAGCATCGAAGAG > NC_002947/4645929‑4646225 | gCCGACTTCCTGCGCGTGGTCAAGCAGATCGATCAACGTCTGGGGCACCACCCGGTGCCCATCCAGCTGGCCATCGGCAGCGAAGAAAACTTCATGGGCCAGATCGACCTGGTGAAGATGAAGGCCATCTACTGGAACGATGCCGACCAg < 1:577011/150‑1 (MQ=255) cTGCGCGTGGTCAAGCAGATCGATCAACGTCTGGGGCACCACCCGGTGCCCATCCAGCCGGCCATCGGCAGCGAAGAAAACTTCATGGGCCAGATCGACCTGGTGAAGATGAAGGCCATCTACTGGAACGATGCCGACCAGGGCACCAGc > 1:172347/1‑150 (MQ=255) aaGCAGATCGATCAACGTCTGGGGCACCACCGGGTGCCCAAGCAGCTGGCCATCGGCAGAGAAAAAAACTACATCCGCTGGAACGGCAGCGACCAGTTCAAGTCTATCTACTGGAACGATGCCGACCAGGGCACCAGCAACAGCgaagaa > 1:408939/1‑150 (MQ=255) ccGGTGCCCATCCAGCTGGCCATCGGCAGCGAAGAAAACTTCATGGGCCAGATCGACCTGGTGAAGATGAAGGCCATCTACTGGAACGATGCCGACCAGGGCACCAGCTACCGCGAAGAAGAAATCCCCGCCGAACTGAAGGCTCTGGcc > 2:722837/1‑150 (MQ=255) gTGCCCATCCAGCTGGCCATCGGCAGCGAAGAAAACTTCATGGGCCAGATCGACCTGGTGAAGATGAAGGCCATCTACTGGAACGATGCCGACCAGGGCACCCGCTACCGCGAAGAAGAAATCCCCGCCGAACTGAAGGCTCTGGCcgac > 1:199619/1‑150 (MQ=255) tGCCCATCCAGCTGGCCATCGGCAGCGAAGAAAACTTCATGGGCCAGATCGACCTGGTGAAGATGAAGGCCATCTACTGGAACGATGCCGACCAGGGCACCAGCTACCGCGAAGAAGAAATCCCCGCCGAACTGAAGGCTCTGGCcgacg < 2:794061/150‑1 (MQ=255) aGCGAAGAAAACTTCATGGGCCAGATCGACCTGGTGAAGATGAAGGCCATCTACTGGAACGATGCCGACCAGGGCACCAGCTACCGCGAAGAAGAAATCCCCGCCGAACTGAAGGCTCTGGCCGACGAATGGCGCGCGCACATGATCGaa > 2:200410/1‑150 (MQ=255) gaagaaAACTTCATGGGCCAGATCGACCTGGTGAAGATGAAGGCCATCTACTGGAACGATGCCGACCAGGGCACCAGCTACCGCGAAGAAGAAATCCCCGCCGAACTGAAGGCTCTGGCCGACGAATGGCGCGCGCACATGATCGAAGCt < 1:387751/150‑1 (MQ=255) agaaAACTTCATGGGCCAGATCGACCTGGTGAAGATGAAGGCCATCTACTGGAACGATGCCGACCAGGGCACCAGCTACCGCGAAGAAGAAATCCCCGCCGCACTGAAGGCTCTGGCCGACGAATGGCGCGCGCACATGATCGAAGCTGc > 1:845063/1‑150 (MQ=255) ttCATGGGCCAGATCGACCTGGTGAAGATG‑AGGCCATCTACTGGAACGATGCCGACCAGGGCACCAGCTACCGCGAAGAAGAAATCCCCGCCGAACTGAAGGCTCTGGCCGACGAATGGCGCGCGCCCATGACCGAAGCTGCGGCCGAgg > 2:773769/1‑150 (MQ=255) cATGGGCCAGATCGACCTGGTGAAGATGAAGGCCATCTACTGGAACGATGCCGACCAGGGCACCAGCTACCGCGAAGAAGAAATCCCCGCCGAACTGAAGGCTCTGGCCGACGAATGGCGCGCGCACATGATCGAAGCTGCGGCCGAGGc < 1:722837/150‑1 (MQ=255) aTCGACCTGGTGAAGATGAAGGCCATCTACTGGAACGATGCCGACCAAGGCACCAGCTACCGCGAAGAAGAAATCCCCGCCGAACTGAAGGCTCTGGCCGACGAATGgc > 2:802411/1‑109 (MQ=255) aTCGACCTGGTGAAGATGAAGGCCATCTACTGGAACGATGCCGACCAAGGCACCAGCTACCGCGAAGAAGAAATCCCCGCCGAACTGAAGGCTCTGGCCGACGAATGgc > 2:596855/1‑109 (MQ=255) aTCGACCTGGTGAAGATGAAGGCCATCTACTGGAACGATGCCGACCAAGGCACCAGCTACCGCGAAGAAGAAATCCCCGCCGAACTGAAGGCTCTGGCCGACGAATGgc < 1:802411/109‑1 (MQ=255) aTCGACCTGGTGAAGATGAAGGCCATCTACTGGAACGATGCCGACCAAGGCACCAGCTACCGCGAAGAAGAAATCCCCGCCGAACTGAAGGCTCTGGCCGACGAATGgc < 1:596855/109‑1 (MQ=255) tGAAGATGAAGGCCATCTACTGGAACGATGCCGACCAGGGCACCAGCTACCGCGAAGAAGAAATCCCCGCCGAACTGAAGGCTCTGGCCGACGAATGGCGCGCGCACATGATCGAAGCTGCGGCCGAGGCCAATGACGAGCTGACCATGa < 2:199619/150‑1 (MQ=255) gAAGATGAAGGCCATCTACTGGAACGATGCCGACCAGGGCACCAGCTACCGCGAAGAAGAAATCCCCGCCGAACTGAAGGCTCTGGCCGACGAATGGCGCGCGCACATGATCGAAGCTGCGGCCGAGGCCAATGACGAGCTGACCATGaa > 2:378469/1‑150 (MQ=255) aaGGCCATCTACTGGAACGATGCCGACCAGGGCACCAGCTACCGCGAAGAAGAAAGCCCCGCCGAACTGAAGGCTCTGGCCGACGAATGGCGCGCGCACATGATCGAAGCTGCGGCCGAGGCCAATGACGAGCTGACCATGAAGTTtctc < 2:408939/150‑1 (MQ=255) gAACGATGCCGACCAGGGCACCAGCTACCGCGAAGAAGAAATCCCCGCCGAACTGAAGGCTCTGGCCGACGAATGGCGCGCGCACATGATCGAAGCTGCGGCCGAGGCCAATGACGAGCTGACCATGAAGttt > 2:474417/1‑133 (MQ=255) gAACGATGCCGACCAGGGCACCAGCTACCGCGAAGAAGAAATCCCCGCCGAACTGAAGGCTCTGGCCGACGAATGGCGCGCGCACATGATCGAAGCTGCGGCCGAGGCCAATGACGAGCTGACCATGAAGttt < 1:474417/133‑1 (MQ=255) gAACGATGCCGACCAGGGCACCAGCTACCGCGAAGAAGAAATCCCCGCCGAACTGAAGGCTCTGGCCGACGAATGGCGCGCGCACATGATCGAAGCTGCGGCCGAGGCCAATGACGAGCTGACCATGAAGTTTCTCGATGGCGAGGAGCt < 2:172347/150‑1 (MQ=255) cAGGGCACCAGCTACCGCGAAGAAGAAATCCCCGCCGAACTGAAGGCTCTGGCCGACGAATGGCGCGCGCACATGATCGAAGCTGCGGCCGAGGCCAATGACGAGCTGACCATGAAGTTTCTCGATGGCGAGGAGCTGAGCATCGAagag > 2:457729/1‑150 (MQ=255) | GCCGACTTCCTGCGCGTGGTCAAGCAGATCGATCAACGTCTGGGGCACCACCCGGTGCCCATCCAGCTGGCCATCGGCAGCGAAGAAAACTTCATGGGCCAGATCGACCTGGTGAAGATGAAGGCCATCTACTGGAACGATGCCGACCAGGGCACCAGCTACCGCGAAGAAGAAATCCCCGCCGAACTGAAGGCTCTGGCCGACGAATGGCGCGCGCACATGATCGAAGCTGCGGCCGAGGCCAATGACGAGCTGACCATGAAGTTTCTCGATGGCGAGGAGCTGAGCATCGAAGAG > NC_002947/4645929‑4646225 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |