| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,665,057 | C→G | 14.2% | R49G (CGC→GGC) | nuoI → | NADH‑quinone oxidoreductase subunit I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,665,057 | 0 | C | G | 14.2% | 88.2 / 9.0 | 36 | R49G (CGC→GGC) | nuoI | NADH‑quinone oxidoreductase subunit I |
| Reads supporting (aligned to +/- strand): ref base C (16/14); new base G (3/2); total (19/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.27e-01 | |||||||||||
ACCCATGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTACCCGGAGTTCTTCCGCA > NC_002947/4664909‑4665198 | aCCCATGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGGTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCg > 1:99994/1‑150 (MQ=255) gCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGAcc > 1:275970/1‑150 (MQ=255) gCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGAcc > 1:565590/1‑150 (MQ=255) gggCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCgagga > 1:606990/1‑150 (MQ=255) gcaccggcaccCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGGTAGCGCGGCGGCag > 2:375033/1‑117 (MQ=255) gcaccggcaccCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGGTAGCGCGGCGGCag < 1:375033/118‑2 (MQ=255) gcaccggcaccCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAgc > 2:327208/1‑150 (MQ=255) ccggcaccCCGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCt < 1:694458/150‑1 (MQ=255) cccgCTGCGCAGCCTGGCATTTGTGTTCTCCCAGGGTTTTTGTAAGCGGGACACCCTGTAATACCCCGAAGAACCCGTGTACCTGCCGTCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGATCCCGATGGCGAGGAGCGCTGCGTAGc < 2:177579/147‑1 (MQ=255) ggTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGc < 1:412585/150‑1 (MQ=255) ctcCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATTGGCAGTAGCTCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGccc < 1:234007/150‑1 (MQ=255) ccACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGt > 2:507534/1‑150 (MQ=255) ccACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGt > 2:291750/1‑150 (MQ=255) cACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCcgc > 1:492642/1‑90 (MQ=255) cACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTg > 2:784564/1‑150 (MQ=255) cACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACATGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCcgc < 2:492642/90‑1 (MQ=255) gggTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGc < 1:817575/150‑1 (MQ=255) ttCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGAt > 1:843632/1‑93 (MQ=255) ttCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGAt < 2:843632/93‑1 (MQ=255) gcgACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGc < 2:521089/150‑1 (MQ=255) gACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACctct < 2:761728/112‑1 (MQ=255) gACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACctct > 1:761728/1‑112 (MQ=255) cacCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCgg < 1:797350/116‑1 (MQ=255) cacCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCgg > 2:797350/1‑116 (MQ=255) cccGTGTACCTTCCGCCGCGCTACCGCGGCCGCAGCGTCCTTACCAGCCGCCACCATCCCGTGGGGCGCTCCGTAGCGTACCGCCTCCGCTCGCTGCCCTGCCAGGTGGGTTGCATTTCGCTGCCGAAGGCCGAGGGAGAGGCCGGCCGc > 2:144070/1‑150 (MQ=255) ccGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCt > 1:217591/1‑150 (MQ=255) tACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTAc < 2:606990/150‑1 (MQ=255) gatgcggccgcGGTAGCGCGGCGGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTAccc > 2:86503/7‑150 (MQ=255) gatGCGGCCGCGGTAGCGCGGCGGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTAccc > 1:498689/3‑150 (MQ=255) cTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTTCAACCCCTGAACACCAGCTTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGGCGGTGGTACCCg > 1:488153/1‑150 (MQ=255) gcggccgcGGTAGCGCGGCGGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTACCCGGa < 2:498689/147‑1 (MQ=255) ccgccgCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCACTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTACCCGGAg < 1:281831/150‑1 (MQ=255) gccgcGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTACCCGGAGtt < 1:784564/150‑1 (MQ=255) gcgcTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCACTTCGCTGCAGCAGGTAGAGACAGAGGGCGGCCGCTGGTATCCGGAGttctt > 2:520893/1‑150 (MQ=255) atcgctGCAGCATAGTCCTCACCCGCGACTCCGATGGCGAGGAGCGCTGCGTAGCGTGCACCCTCTGCGCGGTGGACGGCCCGGATGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTACCCGGAGTTCTTTCGCa < 2:95646/148‑1 (MQ=255) aCCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTACCCGGAGTTCTTCCGCa < 2:696017/150‑1 (MQ=255) | ACCCATGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTACCCGGAGTTCTTCCGCA > NC_002947/4664909‑4665198 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |