| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,672,979 | C→A | 20.0% | V26L (GTG→TTG) | PP_4136 ← | LuxR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,672,979 | 0 | C | A | 20.0% | 53.5 / 6.4 | 25 | V26L (GTG→TTG) | PP_4136 | LuxR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (10/10); new base A (3/2); total (13/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
GCCTTGATCACAGCAGCCACCCGGGTAGGCACTTCAAATTTGCGCAGGATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGG > NC_002947/4672837‑4673121 | gCCTTGATCACATCAGCCACCCGGGTAGGCACTTCACATTTGCGCAGGATGTTGGATAGGTGAAAATTCACCGTTGACTCCTTGCATTCAAGGATCTGGCCGATCTCCTACGACCTCTTGCCATAAGCACACCACAACAGCACTTGCTGt > 2:71299/1‑150 (MQ=255)gCCTTGATCACAGCAGCCACCCGGGTAGGCACTTCAAATTTGCGCAGGATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAACTCTTGCGAGAATCACGCCACAACAGCACTTCCTGt > 2:649073/1‑150 (MQ=255) ccTTGATCACAGCAGCCACTCGGGTAGGCACTTCAAATTTGCGCAGGATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCAAGTGCTGtt > 1:227550/1‑150 (MQ=255) aTCACAGCAGCCACTCGGGTAGGCACTTCAAATTTGCGCAGGATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCAAGTGCTGttgtggt < 2:227550/150‑6 (MQ=255) agcagcCACCCGGGTAGGCACTTCAAATTTGCGCAGGATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGt > 2:808680/1‑150 (MQ=255) aCCCGGGTAGGCACTTCAAATTTGCGCAGGATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCAAGTGCTGttgt > 1:300443/1‑133 (MQ=255) aCCCGGGTAGGCACTTCAAATTTGCGCAGGATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCAAGTGCTGttgt < 2:300443/135‑3 (MQ=255) ggTAGGCACTTCAAATTTGCGCAGGATGTTGTATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCCATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCgg < 1:71299/150‑1 (MQ=255) ttCAAATTTGCGCAGGATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGc > 1:837858/1‑150 (MQ=255) gcgcAGGATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTtg < 2:837858/150‑1 (MQ=255) aGGATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGTATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTtgctgc < 1:649073/150‑1 (MQ=255) aaTTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCTGGGcc > 1:789190/1‑112 (MQ=255) aaTTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCTGGGcc < 2:789190/113‑2 (MQ=255) aTTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTgtcagt > 1:187812/1‑150 (MQ=255) cACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCAt > 2:170068/1‑150 (MQ=255) aacggagACTTCTTGCATTCAAGGATCTGGCCTGTCTCCCACGGCCGTTTTCGATAAACACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGGTGCGGCGAGTGTCGAGCGTTGTCATTCATg > 2:335701/7‑150 (MQ=255) gTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGttt < 1:808680/150‑1 (MQ=255) gACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGaa < 2:505034/150‑1 (MQ=255) aTCTGCCCGATCTCTCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGc < 1:335701/150‑1 (MQ=255) ccGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCAc > 1:614441/1‑132 (MQ=255) ccGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCAc < 2:614441/132‑1 (MQ=255) ctcCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATtgct < 2:221833/150‑1 (MQ=255) gAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTAcc > 2:1838/1‑150 (MQ=255) caacaGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAg < 1:513920/150‑1 (MQ=255) aacaGCAAGTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCAAGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAgg > 1:479998/1‑150 (MQ=255) | GCCTTGATCACAGCAGCCACCCGGGTAGGCACTTCAAATTTGCGCAGGATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGG > NC_002947/4672837‑4673121 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |